LOCUS       sequence-324890-        8364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human codon optimized HSD17B10. Expression is controlled by 
            doxycycline..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Condon KJ, Orozco JM, Adelmann CH, Spinelli JB, van der Helm PW, 
            Roberts JM, Kunchok T, Sabatini DM
  TITLE     Genome-wide CRISPR screens reveal multitiered mechanisms through 
            which mTORC1 senses mitochondrial dysfunction.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2022120118. doi: 
            10.1073/pnas.2022120118.
  PUBMED    33483422
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-01-26"; volume: "118(4). pii: 
            2022120118. doi"; pages: " 10.1073/pnas.2022120118"
COMMENT     Sequence Label: pCW57.kc1-HSD17B10
FEATURES             Location/Qualifiers
     source          1..8364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1929..2046
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2110..2128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2146..2164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2182..2200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2218..2236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2254..2272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2290..2308
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2353..2377
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3317..3382
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3389..3899
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3818..3836
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3909..4301
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MVPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             4311..4364
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4365..4982,4983..4988,4989..5024,5025..5027,5028..5063,
                     5064..5066,5067..5102,5103..5111)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=tTA-Advanced
                     /note="In the Tet-Off(R) system, tTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5151..5171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5569..5580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5785..6018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6090..6211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6127..6146)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6181..6200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6251..6386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6386..6457
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6458..6526,6527..7318)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6676..6695)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7489..8077
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7978..7997
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8231..8248
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggacaa ttctcgacct cgagacaaat ggcagtattc
     1921 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1981 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     2041 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcgaat cgacgtatga
     2101 agagtttact ccctatcagt gatagagaac gtatgcagac tttactccct atcagtgata
     2161 gagaacgtat aaggagttta ctccctatca gtgatagaga acgtatgacc agtttactcc
     2221 ctatcagtga tagagaacgt atctacagtt tactccctat cagtgataga gaacgtatat
     2281 ccagtttact ccctatcagt gatagagaac gtataagctt taggcgtgta cggtgggcgc
     2341 ctataaaagc agagctcgtt tagtgaaccg tcagatcgcc tggagcaatt ccacaacact
     2401 tttgtcttat accaactttc cgtaccactt cctaccctcg taaagtcagt tggccaggcc
     2461 tagttggcca ggcctagtgc cgccaccgtc gacgatggcg gctgcctgta ggagcgtgaa
     2521 gggcctcgta gctgttatta ccgggggcgc ctctggtctc ggtctcgcta cggcagagcg
     2581 acttgtagga cagggggcat ctgccgtatt gctcgacctt cctaattcag gtggtgaggc
     2641 ccaagcgaaa aaactgggta ataactgcgt attcgcaccg gctgacgtga cctctgaaaa
     2701 agatgtgcaa acagcactgg cgctggctaa aggaaaattc ggccgagtgg acgttgctgt
     2761 aaactgcgcc ggcattgcgg tcgcaagcaa gacttacaac ctcaagaaag gccagaccca
     2821 cacgttggaa gacttccaga gggtactcga cgtaaacctg atgggtacat tcaatgtaat
     2881 acgcctcgta gccggcgaaa tgggtcaaaa cgagccggat cagggtggac aacgcggggt
     2941 cattattaac acggcttccg tcgcggcatt tgaaggacag gtgggacaag cagcatactc
     3001 tgcttccaag ggaggcatcg ttggtatgac attgcctatc gcacgggacc tggccccgat
     3061 agggataagg gtaatgacga ttgcaccggg ccttttcgga accccgctgc tcactagtct
     3121 cccagagaaa gtgtgcaact tccttgcaag ccaggttcca ttcccgtcac gactcggaga
     3181 ccctgccgag tatgcacacc ttgtacaagc tataattgag aatcctttcc tgaacgggga
     3241 agttattcga cttgatggag caataagaat gcagccgtga tgaggatcca cgtcgggtgg
     3301 tggtggtggg aatctcgact acaaagacca tgacggtgat tataaagatc atgacatcga
     3361 ttacaaggat gacgatgaca aggattgagg ggttggggtt gcgccttttc caaggcagcc
     3421 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     3481 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     3541 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     3601 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     3661 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     3721 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     3781 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     3841 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagc
     3901 aattcaccat ggttcctttg tctcaagaag aatccaccct cattgaaaga gcaacggcta
     3961 caatcaacag catccccatc tctgaagact acagcgtcgc cagcgcagct ctctctagcg
     4021 acggccgcat cttcactggt gtcaatgtat atcattttac tgggggacct tgtgcagaac
     4081 tcgtggtgct gggcactgct gctgctgcgg cagctggcaa cctgacttgt atcgtcgcga
     4141 tcggaaatga gaacaggggc atcttgagcc cctgcggacg gtgccgacag gtgcttctcg
     4201 atctgcatcc tgggatcaaa gccatagtga aggacagtga tggacagccg acggcagttg
     4261 ggattcgtga attgctgccc tctggttatg tgtgggaggg ccccggtgcc gaaggtagag
     4321 gttctctcct cacttgtggt gatgttgaag aaaaccctgg tccaatgtct agactggaca
     4381 agagcaaagt cataaactct gctctggaat tactcaatga agtcggtatc gaaggcctga
     4441 cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga
     4501 agaacaagcg ggccctgctc gatgccctgg caatcgagat gctggacagg catcataccc
     4561 acttctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat
     4621 tccgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa
     4681 cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct
     4741 ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat
     4801 tggaggatca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta
     4861 tgcccccact tctgagacaa gcaattgagc tgttcgacca tcagggagcc gaacctgcct
     4921 tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg
     4981 gcgggccggc cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg
     5041 acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc
     5101 tccccgggta actaagtaag gatcgatcca agatatcgta ttcttaacta tgttgctcct
     5161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     5221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     5281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     5341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     5401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     5461 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     5521 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     5581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     5641 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgt ttcgcctcgg
     5701 cgtccggact agaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6001 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6061 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6241 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6301 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6361 gtagtgagga ggcttttttg gaggcctttc aaatatgtat ccgctcatga gacaataacc
     6421 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6481 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6541 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6601 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6661 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6721 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6781 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6841 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6901 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6961 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     7021 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     7081 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     7141 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     7201 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     7261 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     7321 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7381 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7441 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7501 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7561 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7621 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7681 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7741 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7801 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7861 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7921 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7981 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8041 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8101 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8161 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     8221 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     8281 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     8341 agcgggcagt gagcgcaacg caat
//
