LOCUS       sequence-32490-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-1224
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..63
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        44..62
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctggctac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
       61 gggagaccca agctggctag ttaagctatc aacaagtttg tacaaaaaag caggctgcag
      121 gtgtggagtt caggtatggt ccttctctga tgacctctaa ggtgtgacac tcacctcact
      181 ggcaagccag tgggactgac tctactctta cagatccagc tgagctctca ggcatgtctg
      241 cctctcaaga ccagctagct gccgctcctc tgtccacagc agtccactct aaaggtaggg
      301 ctcaaatgca gtggattggg cacagatggg ctttggacca cttgctggta ctgggtgacc
      361 ctactgtagg acatcaggga ggctgttctg caggtgcctg ggacctggac ctaaatgaca
      421 actccaagtc agcattgggg gagcccatat ctcctactgg agatcatcat ggcttgcccc
      481 accagcctcc agcctcttcc aggctcagcc tgggtcgtca tcgaagactc tgcagctcca
      541 ataagggcca gactcatgag aacagcaatg acggcagcaa ccatgactac ctgcctctgg
      601 tgaggactgg ggaggtggag ggtagcatca ttagagccag agctctgtct cagctccctc
      661 tccccccacc tcttctctcc tcaggtacga ctgcaagagg caccaggctc cttccgcctg
      721 gatgaaccct tctgtgctgc tgtgtgcatt cctcaagaac gcctgtgtcg agcctcgccc
      781 tttgccgcac accgtgccag cctcagcccc acctcagcat catctccttg ggcatttctg
      841 agccctggta ttggccaagg tgacagtgcc a
//
