LOCUS       sequence-324901-        9946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heart-specific tamoxifen-dependent Cre expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Chin HJ, Lee SY, Lee D
  TITLE     Tamoxifen-inducible cardiac-specific Cre transgenic mouse using 
            VIPR2 intron.
  JOURNAL   Lab Anim Res. 2020 Sep 15;36:31. doi: 10.1186/s42826-020-00065-x. 
            eCollection 2020.
  PUBMED    32983955
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Lab Anim 
            Res."; date: "2020-09-15"; volume: "36:31. doi"; pages: " 
            10.1186/s42826-020-00065-x. eCollection 2020"
COMMENT     Sequence Label: pVIPR2-ERT2CreERT2pA
FEATURES             Location/Qualifiers
     source          1..9946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      6164..6173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6173..7108
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             7118..8140
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             8153..9088
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             8201..9094
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    9140..9261
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9177..9196)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9231..9250
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9305..9324)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9306..9324)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9331..9348)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9331..9347)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9340..9362)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9489..9944
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9576..9595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9786..9807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggagctccac cgcggtggcg
     2221 gccgctggga ggagaagggc tctgcggacc agagggctct agggcagccc tgggcactgc
     2281 tgagcagctg catctcatct ggacctggag cccaaggggc cgcaccagcc gtggccatgg
     2341 tctgggcctt tatttggggt ctcacctgct gcagtcagat cctgaagcag ataagccagc
     2401 cagggatcag tctccctgtc caaggtcttc ccgtctgtgt cctcttcctg ccaagaccag
     2461 ctttcaggcc tgggggagtc ttaggtcttc actgagtaaa gggtttcatc cacagtgtat
     2521 caaggagcaa tggccccagt ccttgccctc cacagctgct cctctccgct tgcccacccg
     2581 cactgtaagc cacagttctc gagcaccacc accagcagac tgagggctcg ggatcaccaa
     2641 gtgtgggagg ctccccgagc cgtgtgctgg ggcccgacca cccggccctg ccctgccctg
     2701 cccttcatcc catcacaact cttctgtgct gatggatggg ggccggggtt cccactgcgt
     2761 ggaaaacaga acagctcagg cagaggacgg gagcggcagt ggccgggctg gtgtgaggct
     2821 ggggattccg cttgccccgg cagggctcag gcttccaaga ggccacggag gagctcctgg
     2881 gactgatcgt ggtgcgggct ctggcccagt gggtctgtgt gggggctgag accctgcctt
     2941 tccagcacgt tcccagctgg tgccagttgg agtcagagcc tggaatcctt acacgagagg
     3001 ccagaagcct tggcctcagc agtccaggcc tgagaaccca cccagctgtc actcaccgat
     3061 ggctgagtga ctttgcacaa agccctgttg cccgccctat cccactcctg ggatcagacc
     3121 cagaaatctt ggtctgtgtg tcccaactgg ctacaattgc tgctcagctt ctgaaaggtg
     3181 cctcttcaag cctgtttctt gaaacaaggc actgaaaagc atacgttgtg tcaaaagccc
     3241 aaaaggagtg ggaattctcc ttggtgattt ggatttcatc ttgggcactg atgaaagagc
     3301 acagagtggg ggcctccctc cttgagtcag ggtcaggcag gggcaaggca gtagaatctc
     3361 taacaccctg ggcactcaca gccctgccat tgcttctgtg gtggaggtgg ccagggcgga
     3421 gtgtggcgtt cccgggaatg gcctcagcag cacaccgtgg tgggcacgcg agggggccgg
     3481 ctcccgtaag tcttctgtgt tacatactgg ttaactccgt tcttacataa agagcacgtc
     3541 tgctaaattt agccaagaga actgtttggc atttcggcct ccccatgctt caagtgcaca
     3601 catttcttcc gtgttacgcc acatccctaa aaataagcct ctgccctcct ccagcactca
     3661 ggccaccgca gtgactcgtt cccactgatg gcaaacttca ctccccaggg ctcccatccc
     3721 tggtgacaaa gatcacagca gctggcactg cagatgcctt tgtgggaggc accatctgat
     3781 catcccgcca accctcctgc aaccctgcaa ggacacacca gcctcccagg gtgccaggag
     3841 gagcctgcca gagaccccca gagcggcctg ccccaaatca cctctgagag cagcagcttt
     3901 tctgaaggaa gcctcccctc atggtcctca ggcccagcag gtgccaagct caatgccagc
     3961 cacgagggca ttggctcatc gagtgatggg aacggggaca gcaaggcggc cacagagagg
     4021 gtggtctcag ccatggacac agtacggcgg aagcatcccg aggtgggccg ggacaagagc
     4081 tgggcctgtg gctgctgcgg gggccacgag actgacctca ggcaggggca gaaatctggc
     4141 cacaggcaga aatcattcag ctgtcacctg gcacaaccag acaggattat gagcactttt
     4201 ctgctgttta gttttaacct gtgcatgtga tgcttaatac tttccaaata aatggatgag
     4261 tcttttcagg gccaaaggag atacgggacc agggaaggag gttaagaagg aaatgcacag
     4321 atacgctggt gcctggggat ggcgattggg tctccaagtt ctttaagttc taaaggaatc
     4381 cgctagggaa gattctccta atgggaggga gatgaaggtg aaggaattag aggtccacat
     4441 gatcaggaag gtggaaggat ggaggagcac accaggatga tgtgcagcac agggcagcgg
     4501 acctgtagga aacacagcgg tgcgccaggc gcttctgggc atggagctgg ccgatgccgg
     4561 aggaggggag gtacgtgcct gacccaggat ggaccatcca aggcagcagg gtctgcacac
     4621 caggctctgc atgaaggagt gtgtggcccc aggcaggggt tgaaactctc cgggcctcga
     4681 tggataggag gcctgtcttt gggcttgggc agccttgggt gggacctccg gcattgtgct
     4741 ctggggaagg gtccagcctc cctgctgctc cccttaaggg gaggtctgta tgtgggcggc
     4801 agatgttcag cttgggcacc cagcagctgg ctcttgggct ccacaggtgg gtggggcagg
     4861 ggcgctggtt ctgagaaggt gtgcgtcctg cagcttgttg ccaggagccc ccggggagcc
     4921 tccagatcat acaccacaca ccacactggc ccctccagga cacacagcac atgcttcagg
     4981 actcagcatt tatggtcctc agaaacaact gtattgaaga aagtgtggaa acccaaattt
     5041 cagggttcta gggaggtctg ttaaagactt aaaaaaaaaa gagagaagga agagttttga
     5101 agggattcac ctactgcctg tcaggtcggc accaactgtc atgtcgatgt gatttggagc
     5161 ctggacagga gcaggggctc gtggagacca ccgtgggctg gataacggcc aagggggcag
     5221 aacagcaggg ccttgagtct ggggcttccg gtgacactcc tgttctcacg cgtgagctcc
     5281 aggaacatcc aaactgagca gccggggtcc cccccacccc ccaccccgcc cctcccggca
     5341 actttgagcc tgtgctggga cagagcctct agttcctaaa ttagtccatg aggtcagagg
     5401 cagcactgcc attgtaacgc gattggagag gatcacgtca ccggacacgc ccccaggcat
     5461 ctccctgggt ctcctaaact tggcggggag aagttttagc ccttaagttt tagcctttaa
     5521 cccccatatt cagaactgtg cgagttggcg aaaccccaca aatcacaaca aactgtacac
     5581 aacaccgagc tagaggtgat ctttcttgtc cattccacac aggccttagt aatgcgtcgc
     5641 catagcaaca gtgtcactag tagcaccagc acttccccac accctccccc tcaggaatcc
     5701 gtactctcca gtgaacccca gaaacctctg gagagttctg gacaagggcg gaacccacaa
     5761 ctccgattac tcaagggagg cggggaagct ccaccagacg cgaaactgct ggaagattcc
     5821 tggccccaag gcctcctccg gctcgctgat tggcccagcg gagagtgggc ggggccggtg
     5881 aagactcctt aaaggcgcag ggcggcgagc aggtcaccag acgctgacag ctactcagaa
     5941 ccaaatctgg ttccatccag agacaagcga agacaagaga agcagagcga gcggcgcgtt
     6001 cccgatcctc ggccaggacc agccttcccc agagcatccc tgccgcggag cgcaaccttc
     6061 ccaggagcat ccctgccgcg gagcgcaact ttccccggag catccacgcc gcggagcgca
     6121 gccttccaga agcagagcgt ctagtggatc ccccgggtga gccgccacca tggctggaga
     6181 catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag
     6241 cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc
     6301 catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt
     6361 actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc
     6421 aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga
     6481 gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc
     6541 tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt
     6601 cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt
     6661 tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac
     6721 cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac
     6781 tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc
     6841 ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct
     6901 gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga
     6961 cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca
     7021 aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac
     7081 gggggaggca gagggtttcc ctgccacagc tgtcgacaat ttactgaccg tacaccaaaa
     7141 tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat
     7201 gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg
     7261 gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga
     7321 tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca
     7381 acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag
     7441 caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg
     7501 tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga
     7561 aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac
     7621 cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg
     7681 tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga
     7741 gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt
     7801 agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc
     7861 atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg
     7921 attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag
     7981 tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat
     8041 gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag
     8101 tgaaacaggg gcaatggtgc gcctgctgga agatggcgat ctcgagccat ctgctggaga
     8161 catgagagct gccaaccttt ggccaagccc gctcatgatc aaacgctcta agaagaacag
     8221 cctggccttg tccctgacgg ccgaccagat ggtcagtgcc ttgttggatg ctgagccccc
     8281 catactctat tccgagtatg atcctaccag acccttcagt gaagcttcga tgatgggctt
     8341 actgaccaac ctggcagaca gggagctggt tcacatgatc aactgggcga agagggtgcc
     8401 aggctttgtg gatttgaccc tccatgatca ggtccacctt ctagaatgtg cctggctaga
     8461 gatcctgatg attggtctcg tctggcgctc catggagcac ccagtgaagc tactgtttgc
     8521 tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta gagggcatgg tggagatctt
     8581 cgacatgctg ctggctacat catctcggtt ccgcatgatg aatctgcagg gagaggagtt
     8641 tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg tacacatttc tgtccagcac
     8701 cctgaagtct ctggaagaga aggaccatat ccaccgagtc ctggacaaga tcacagacac
     8761 tttgatccac ctgatggcca aggcaggcct gaccctgcag cagcagcacc agcggctggc
     8821 ccagctcctc ctcatcctct cccacatcag gcacatgagt aacaaaggca tggagcatct
     8881 gtacagcatg aagtgcaaga acgtggtgcc cctctatgac ctgctgctgg aggcggcgga
     8941 cgcccaccgc ctacatgcgc ccactagccg tggaggggca tccgtggagg agacggacca
     9001 aagccacttg gccactgcgg gctctacttc atcgcattcc ttgcaaaagt attacatcac
     9061 gggggaggca gagggtttcc ctgccacagc ttgatgaaga tctgagctcc ctggcggaat
     9121 tcggatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca aataaagcaa
     9181 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9241 caaactcatc aatgtatctt atcatgtctg gtcgacctcg agggggggcc cggtacccaa
     9301 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     9361 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     9421 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     9481 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     9541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     9601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     9661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     9721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     9781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     9841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     9901 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttag
//
