LOCUS       sequence-324908-       13141 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses LRRK2 tagged with APEX2 and 3xFLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Bonet-Ponce L, Beilina A, Williamson CD, Lindberg E, Kluss JH, 
            Saez-Atienzar S, Landeck N, Kumaran R, Mamais A, Bleck CKE, Li Y, 
            Cookson MR
  TITLE     LRRK2 mediates tubulation and vesicle sorting from lysosomes.
  JOURNAL   Sci Adv. 2020 Nov 11;6(46). pii: 6/46/eabb2454. doi: 
            10.1126/sciadv.abb2454. Print 2020 Nov.
  PUBMED    33177079
REFERENCE   2  (bases 1 to 13141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-11-11"; volume: "6(46). pii: 6/46/eabb2454. doi"; pages:
            " 10.1126/sciadv.abb2454. Print 2020 Nov"
COMMENT     Sequence Label: 3xflag-APEX2-LRRK2
FEATURES             Location/Qualifiers
     source          1..13141
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     146..168
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     160..177
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     161..177
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        338..717
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        718..921
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     872..892
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             951..1016
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1017..1763
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     protein_bind    1803..1827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9444..9468)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    9502..10124
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(9701..9720)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(10148..10168)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10153..10482
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10333..10468
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10520..10539)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10521..10539)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10553..10569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10553..10569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10566..10588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10577..10593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10601..10607,10608..10625,10626..10631))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10646..10667
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10784..10801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10955..11543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11035..11054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11714..12505,12506..12574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12337..12356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12575..12679)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12706..13134
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12793..12812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13003..13024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
       61 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      121 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      181 aagctgatct atacattgaa tcaatattgg caattagcca tattagtcat tggttatata
      241 gcataaatca atattggcta ttggccattg catacgttgt atctatatca taatatgtac
      301 atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt gactagttat
      361 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      421 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      481 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      541 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtccg
      601 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      661 ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
      721 atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg gggatttcca
      781 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
      841 ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg
      901 gaggtctata taagcagagc tcgtttagtg aaccgtcaga attaaccatg gactacaaag
      961 accatgacgg tgattataaa gatcatgaca tcgattacaa ggatgacgat gacaagggaa
     1021 agtcttaccc aactgtgagt gctgattacc aggacgccgt tgagaaggcg aagaagaagc
     1081 tcagaggctt catcgctgag aagagatgcg ctcctctaat gctccgtttg gcattccact
     1141 ctgctggaac ctttgacaag ggcacgaaga ccggtggacc cttcggaacc atcaagcacc
     1201 ctgccgaact ggctcacagc gctaacaacg gtcttgacat cgctgttagg cttttggagc
     1261 cactcaaggc ggagttccct attttgagct acgccgattt ctaccagttg gctggcgttg
     1321 ttgccgttga ggtcacgggt ggacctaagg ttccattcca ccctggaaga gaggacaagc
     1381 ctgagccacc accagagggt cgcttgcccg atcccactaa gggttctgac catttgagag
     1441 atgtgtttgg caaagctatg gggcttactg accaagatat cgttgctcta tctgggggtc
     1501 acactattgg agctgcacac aaggagcgtt ctggatttga gggtccctgg acctctaatc
     1561 ctcttatttt cgacaactca tacttcacgg agttgttgag tggtgagaag gaaggtctcc
     1621 ttcagctacc ttctgacaag gctcttttgt ctgaccctgt attccgccct ctcgttgaca
     1681 aatatgcagc ggacgaagat gccttctttg ctgattacgc tgaggctcac caaaagcttt
     1741 ccgagcttgg gtttgctgat gccaagcttg cggccgcgaa ttcatcgata gatctgatat
     1801 caacaagttt gtacaaaaaa gcaggctccg aattcgccct tatggctagt ggcagctgtc
     1861 aggggtgcga agaggacgag gaaactctga agaagttgat agtcaggctg aacaatgtcc
     1921 aggaaggaaa acagatagaa acgctggtcc aaatcctgga ggatctgctg gtgttcacgt
     1981 actccgagca cgcctccaag ttatttcaag gcaaaaatat ccatgtgcct ctgttgatcg
     2041 tcttggactc ctatatgaga gtcgcgagtg tgcagcaggt gggttggtca cttctgtgca
     2101 aattaataga agtctgtcca ggtacaatgc aaagcttaat gggaccccag gatgttggaa
     2161 atgattggga agtccttggt gttcaccaat tgattcttaa aatgctaaca gttcataatg
     2221 ccagtgtaaa cttgtcagtg attggactga agaccttaga tctcctccta acttcaggta
     2281 aaatcacctt gctgatattg gatgaagaaa gtgatatttt catgttaatt tttgatgcca
     2341 tgcactcatt tccagccaat gatgaagtcc agaaacttgg atgcaaagct ttacatgtgc
     2401 tgtttgagag agtctcagag gagcaactga ctgaatttgt tgagaacaaa gattatatga
     2461 tattgttaag tgcgttaaca aattttaaag atgaagagga aattgtgctt catgtgctgc
     2521 attgtttaca ttccctagcg attccttgca ataatgtgga agtcctcatg agtggcaatg
     2581 tcaggtgtta taatattgtg gtggaagcta tgaaagcatt ccctatgagt gaaagaattc
     2641 aagaagtgag ttgctgtttg ctccataggc ttacattagg taattttttc aatatcctgg
     2701 tattaaacga agtccatgag tttgtggtga aagctgtgca gcagtaccca gagaatgcag
     2761 cattgcagat ctcagcgctc agctgtttgg ccctcctcac tgagactatt ttcttaaatc
     2821 aagatttaga ggaaaagaat gagaatcaag agaatgatga tgagggggaa gaagataaat
     2881 tgttttggct ggaagcctgt tacaaagcat taacgtggca tagaaagaac aagcacgtgc
     2941 aggaggccgc atgctgggca ctaaataatc tccttatgta ccaaaacagt ttacatgaga
     3001 agattggaga tgaagatggc catttcccag ctcataggga agtgatgctc tccatgctga
     3061 tgcattcttc atcaaaggaa gttttccagg catctgcgaa tgcattgtca actctcttag
     3121 aacaaaatgt taatttcaga aaaatactgt tatcaaaagg aatacacctg aatgttttgg
     3181 agttaatgca gaagcatata cattctcccg aagtggctga aagtggctgt aaaatgctaa
     3241 atcatctttt tgaaggaagc aacacttccc tggatataat ggcagcagtg gtccccaaaa
     3301 tactaacagt tatgaaacgt catgagacat cattaccagt gcagctggag gcgcttcgag
     3361 ctattttaca ttttatagtg cctggcatgc cagaagaatc cagggaggat acagaatttc
     3421 atcataagct aaatatggtt aaaaaacagt gtttcaagaa tgatattcac aaactggtcc
     3481 tagcagcttt gaacaggttc attggaaatc ctgggattca gaaatgtgga ttaaaagtaa
     3541 tttcttctat tgtacatttt cctgatgcat tagagatgtt atccctggaa ggtgctatgg
     3601 attcagtgct tcacacactg cagatgtatc cagatgacca agaaattcag tgtctgggtt
     3661 taagtcttat aggatacttg attacaaaga agaatgtgtt cataggaact ggacatctgc
     3721 tggcaaaaat tctggtttcc agcttatacc gatttaagga tgttgctgaa atacagacta
     3781 aaggatttca gacaatctta gcaatcctca aattgtcagc atctttttct aagctgctgg
     3841 tgcatcattc atttgactta gtaatattcc atcaaatgtc ttccaatatc atggaacaaa
     3901 aggatcaaca gtttctaaac ctctgttgca agtgttttgc aaaagtagct atggatgatt
     3961 acttaaaaaa tgtgatgcta gagagagcgt gtgatcagaa taacagcatc atggttgaat
     4021 gcttgcttct attgggagca gatgccaatc aagcaaagga gggatcttct ttaatttgtc
     4081 aggtatgtga gaaagagagc agtcccaaat tggtggaact cttactgaat agtggatctc
     4141 gtgaacaaga tgtacgaaaa gcgttgacga taagcattgg gaaaggtgac agccagatca
     4201 tcagcttgct cttaaggagg ctggccctgg atgttgccaa caatagcatt tgccttggag
     4261 gattttgtat aggaaaagtt gaaccttctt ggcttggtcc tttatttcca gataagactt
     4321 ctaatttaag gaaacaaaca aatatagcat ctacactagc aagaatggtg atcagatatc
     4381 agatgaaaag tgctgtggaa gaaggaacag cctcaggcag cgatggaaat ttttctgaag
     4441 atgtgctgtc taaatttgat gaatggacct ttattcctga ctcttctatg gacagtgtgt
     4501 ttgctcaaag tgatgacctg gatagtgaag gaagtgaagg ctcatttctt gtgaaaaaga
     4561 aatctaattc aattagtgta ggagaatttt accgagatgc cgtattacag cgttgctcac
     4621 caaatttgca aagacattcc aattccttgg ggcccatttt tgatcatgaa gatttactga
     4681 agcgaaaaag aaaaatatta tcttcagatg attcactcag gtcatcaaaa cttcaatccc
     4741 atatgaggca ttcagacagc atttcttctc tggcttctga gagagaatat attacatcac
     4801 tagacctttc agcaaatgaa ctaagagata ttgatgccct aagccagaaa tgctgtataa
     4861 gtgttcattt ggagcatctt gaaaagctgg agcttcacca gaatgcactc acgagctttc
     4921 cacaacagct atgtgaaact ctgaagagtt tgacacattt ggacttgcac agtaataaat
     4981 ttacatcatt tccttcttat ttgttgaaaa tgagttgtat tgctaatctt gatgtctctc
     5041 gaaatgacat tggaccctca gtggttttag atcctacagt gaaatgtcca actctgaaac
     5101 agtttaacct gtcatataac cagctgtctt ttgtacctga gaacctcact gatgtggtag
     5161 agaaactgga gcagctcatt ttagaaggaa ataaaatatc agggatatgc tcccccttga
     5221 gactgaagga actgaagatt ttaaacctta gtaagaacca catttcatcc ctatcagaga
     5281 actttcttga ggcttgtcct aaagtggaga gtttcagtgc cagaatgaat tttcttgctg
     5341 ctatgccttt cttgcctcct tctatgacaa tcctaaaatt atctcagaac aaattttcct
     5401 gtattccaga agcaatttta aatcttccac acttgcggtc tttagatatg agcagcaatg
     5461 atattcagta cctaccaggt cccgcacact ggaaatcttt gaacttaagg gaactcttat
     5521 ttagccataa tcagatcagc atcttggact tgagtgaaaa agcatattta tggtctagag
     5581 tagagaaact gcatctttct cacaataaac tgaaagagat tcctcctgag attggctgtc
     5641 ttgaaaatct gacatctctg gatgtcagtt acaacttgga actaagatcc tttcccaatg
     5701 aaatggggaa attaagcaaa atatgggatc ttcctttgga tgaactgcat cttaactttg
     5761 attttaaaca tataggatgt aaagccaaag acatcataag gtttcttcaa cagcgattaa
     5821 aaaaggctgt gccttataac cgaatgaaac ttatgattgt gggaaatact gggagtggta
     5881 aaaccacctt attgcagcaa ttaatgaaaa ccaagaaatc agatcttgga atgcaaagtg
     5941 ccacagttgg catagatgtg aaagactggc ctatccaaat aagagacaaa agaaagagag
     6001 atctcgtcct aaatgtgtgg gattttgcag gtcgtgagga attctatagt actcatcccc
     6061 attttatgac gcagcgagca ttgtaccttg ctgtctatga cctcagcaag ggacaggctg
     6121 aagttgatgc catgaagcct tggctcttca atataaaggc tcgcgcttct tcttcccctg
     6181 tgattctcgt tggcacacat ttggatgttt ctgatgagaa gcaacgcaaa gcctgcatga
     6241 gtaaaatcac caaggaactc ctgaataagc gagggttccc tgccatacga gattaccact
     6301 ttgtgaatgc caccgaggaa tctgatgctt tggcaaaact tcggaaaacc atcataaacg
     6361 agagccttaa tttcaagatc cgagatcagc ttgttgttgg acagctgatt ccagactgct
     6421 atgtagaact tgaaaaaatc attttatcgg agcgtaaaaa tgtgccaatt gaatttcccg
     6481 taattgaccg gaaacgatta ttacaactag tgagagaaaa tcagctgcag ttagatgaaa
     6541 atgagcttcc tcacgcagtt cactttctaa atgaatcagg agtccttctt cattttcaag
     6601 acccagcact gcagttaagt gacttgtact ttgtggaacc caagtggctt tgtaaaatca
     6661 tggcacagat tttgacagtg aaagtggaag gttgtccaaa acaccctaag ggaattattt
     6721 cgcgtagaga tgtggaaaaa tttctttcaa aaaaaaggaa atttccaaag aactacatgt
     6781 cacagtattt taagctccta gaaaaattcc agattgcttt gccaatagga gaagaatatt
     6841 tgctggttcc aagcagtttg tctgaccaca ggcctgtgat agagcttccc cattgtgaga
     6901 actctgaaat tatcatccga ctatatgaaa tgccttattt tccaatggga ttttggtcaa
     6961 gattaatcaa tcgattactt gagatttcac cttacatgct ttcagggaga gaacgagcac
     7021 ttcgcccaaa cagaatgtat tggcgacaag gcatttactt aaattggtct cctgaagctt
     7081 attgtctggt aggatctgaa gtcttagaca atcatccaga gagtttctta aaaattacag
     7141 ttccttcttg tagaaaaggc tgtattcttt tgggccaagt tgtggaccac attgattctc
     7201 tcatggaaga atggtttcct gggttgctgg agattgatat ttgtggtgaa ggagaaactc
     7261 tgttgaagaa atgggcatta tatagtttta atgatggcga agaacatcaa aaaatcttac
     7321 ttgatgactt gatgaagaaa gcagaggaag gagatctctt agtaaatcca gatcaaccaa
     7381 ggctcaccat tccaatatct cagattgccc ctgacttgat tttggctgac ctgcctagaa
     7441 atattatgtt gaataatgat gagttggaat ttgaacaagc tccagagttt ctcctaggtg
     7501 atggcagttt tggatcagtt taccgagcag cctatgaagg agaagaagtg gctgtgaaga
     7561 tttttaataa acatacatca ctcaggctgt taagacaaga gcttgtggtg ctttgccacc
     7621 tccaccaccc cagtttgata tctttgctgg cagctgggat tcgtccccgg atgttggtga
     7681 tggagttagc ctccaagggt tccttggatc gcctgcttca gcaggacaaa gccagcctca
     7741 ctagaaccct acagcacagg atcgcactcc acgtagctga tggtttgaga tacctccact
     7801 cagccatgat tatataccga gacctgaaac cccacaatgt gctgcttttc acactgtatc
     7861 ccaatgctgc catcatcgca aagattgctg actacggcat tgctcagtac tgctgtagaa
     7921 tggggataaa aacatcagag ggcacaccag ggtttcgtgc acctgaagtt gccagaggaa
     7981 atgtcattta taaccaacag gctgatgttt attcatttgg tttactactc tatgacattt
     8041 tgacaactgg aggtagaata gtagagggtt tgaagtttcc aaatgagttt gatgaattag
     8101 aaatacaagg aaaattacct gatccagtta aagaatatgg ttgtgcccca tggcctatgg
     8161 ttgagaaatt aattaaacag tgtttgaaag aaaatcctca agaaaggcct acttctgccc
     8221 aggtctttga cattttgaat tcagctgaat tagtctgtct gacgagacgc attttattac
     8281 ctaaaaacgt aattgttgaa tgcatggttg ctacacatca caacagcagg aatgcaagca
     8341 tttggctggg ctgtgggcac accgacagag gacagctctc atttcttgac ttaaatactg
     8401 aaggatacac ttctgaggaa gttgctgata gtagaatatt gtgcttagcc ttggtgcatc
     8461 ttcctgttga aaaggaaagc tggattgtgt ctgggacaca gtctggtact ctcctggtca
     8521 tcaataccga agatgggaaa aagagacata ccctagaaaa gatgactgat tctgtcactt
     8581 gtttgtattg caattccttt tccaagcaaa gcaaacaaaa aaattttctt ttggttggaa
     8641 ccgctgatgg caagttagca atttttgaag ataagactgt taagcttaaa ggagctgctc
     8701 ctttgaagat actaaatata ggaaatgtca gtactccatt gatgtgtttg agtgaatcca
     8761 caaattcaac ggaaagaaat gtaatgtggg gaggatgtgg cacaaagatt ttctcctttt
     8821 ctaatgattt caccattcag aaactcattg agacaagaac aagccaactg ttttcttatg
     8881 cagctttcag tgattccaac atcataacag tggtggtaga cactgctctc tatattgcta
     8941 agcaaaatag ccctgttgtg gaagtgtggg ataagaaaac tgaaaaactc tgtggactaa
     9001 tagactgcgt gcacttttta agggaggtaa tggtaaaaga aaacaaggaa tcaaaacaca
     9061 aaatgtctta ttctgggaga gtgaaaaccc tctgccttca gaagaacact gctctttgga
     9121 taggaactgg aggaggccat attttactcc tggatctttc aactcgtcga cttatacgtg
     9181 taatttacaa cttttgtaat tcggtcagag tcatgatgac agcacagcta ggaagcctta
     9241 aaaatgtcat gctggtattg ggctacaacc ggaaaaatac tgaaggtaca caaaagcaga
     9301 aagagataca atcttgcttg accgtttggg acatcaatct tccacatgaa gtgcaaaatt
     9361 tagaaaaaca cattgaagtg agaaaagaat tagctgaaaa aatgagacga acatctgttg
     9421 agtaagagag aagggcgaat tcgacccagc tttcttgtac aaagtggttg atatcggtac
     9481 cagtcgactc tagaggatcc cgggtggcat ccctgtgacc cctccccagt gcctctcctg
     9541 gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt aagttgcatc
     9601 attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt ggtatggagc
     9661 aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga accaagctgg
     9721 agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca agcgattctc
     9781 ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct cagctaattt
     9841 ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc caactcctaa
     9901 tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc gtgaaccact
     9961 gctcccttcc ctgtccttct gattttaaaa taactatacc agcaggagga cgtccagaca
    10021 cagcataggc tacctggcca tgcccaaccg gtgggacatt tgagttgctt gcttggcact
    10081 gtcctctcat gcgttgggtc cactcagtag atgcctgttg aattgggtac gcggccagct
    10141 tggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
    10201 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
    10261 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
    10321 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10381 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10441 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctcct cgaggaactg
    10501 aaaaaccaga aagttaattc cctatagtga gtcgtattaa attcgtaatc atgtcatagc
    10561 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    10621 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    10681 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    10741 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
    10801 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
    10861 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
    10921 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
    10981 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
    11041 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
    11101 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
    11161 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
    11221 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
    11281 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
    11341 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
    11401 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
    11461 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
    11521 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
    11581 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
    11641 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
    11701 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
    11761 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
    11821 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
    11881 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
    11941 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
    12001 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
    12061 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
    12121 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
    12181 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
    12241 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
    12301 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
    12361 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    12421 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    12481 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    12541 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    12601 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
    12661 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg
    12721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    12781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    12841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    12901 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    12961 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    13021 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    13081 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
    13141 a
//
