LOCUS       sequence-32492-e         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-1197
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..119
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(475..499)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             525..566
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(606..625)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    650..698
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gactctctgg ctactgagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagttaagct atcaacaagt ttgtacaaaa aagcaggcta
      121 catgagtttg tggccattgc cacacatgcc gattgctggt agtttgtgac actcattgag
      181 aaggcatttt ggaggtaaca ccaaattaag taaagtgagc tggaatcagc cagcgttacc
      241 tcaaggtatt tgaagatgcg gttgaccatg gtgtgtacgc tttatttatg acgtaggaca
      301 catggtctac ttcttctcaa tatcacatct cgccttggaa gacttccagg aggtgacatc
      361 tgctttgagg aagagccagt gtcccggtct tcctactgct gctgcttggt acttggagag
      421 aggtggtccg tggcgcgttc gcttcattta tggcgcacat tacacggtcg acctacccag
      481 ctttcttgta caaagtggtt gatctagagg gcccgcggtt cgaaggtaag cctatcccta
      541 accctctcct cggtctcgat tctacgcgta ccggttagta atgagtttaa acgggggagg
      601 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
      661 agacagaata aaacgcacgg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
      721 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
      781 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
      841 cggggcgcca ggccctgcca tagcagatct gcgcagctgg ggctctaggg ggtatcccca
      901 cgcgcc
//
