LOCUS       sequence-324924-         303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting
            the human gene TBK1. Contains a puro resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
  TITLE     Nuclear sensing of breaks in mitochondrial DNA enhances immune 
            surveillance.
  JOURNAL   Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w.
            Epub 2021 Feb 24.
  PUBMED    33627873
REFERENCE   2  (bases 1 to 303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03269-w"; journalName: "Nature"; date: 
            "2021-03"; volume: "591"; issue: "7850"; pages: "477-481"
COMMENT     Sequence Label: pLKO.1 puro_shTBK1_3185
FEATURES             Location/Qualifiers
     source          1..303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    134..251
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cttggcttta tatatcttgt ggaaaggacg aaacaccggg cggcagagtt aggtgaaatt
       61 ctcgagaatt tcacctaact ctgccgcttt ttgaattctc gacctcgaga caaatggcag
      121 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      181 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      241 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg cggctcgagg
      301 ggg
//
