LOCUS       sequence-324927-        1041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral plKO.1 construct coding for a short-hairpin RNA targeting
            the human gene DDX58 (coding for RLR sensor RIG-I). Contains a puro 
            resistance cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1041)
  AUTHORS   Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
  TITLE     Nuclear sensing of breaks in mitochondrial DNA enhances immune 
            surveillance.
  JOURNAL   Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w.
            Epub 2021 Feb 24.
  PUBMED    33627873
REFERENCE   2  (bases 1 to 1041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03269-w"; journalName: "Nature"; date: 
            "2021-03"; volume: "591"; issue: "7850"; pages: "477-481"
COMMENT     Sequence Label: pLKO.1 puro_shDDX58_230212
FEATURES             Location/Qualifiers
     source          1..1041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    136..253
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        302..812
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     731..749
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(834..853)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 atttnngnnt ttatatatct tgtggaagga cgaaacaccg gagcacttgt ggacgcttta
       61 aactnnngnt taaagcgtcc acaagtgctt ttttgaattc tcgacctcga gacaaatggc
      121 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      181 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      241 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc cgcggctcga
      301 gggggttggg gttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
      361 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
      421 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
      481 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
      541 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
      601 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
      661 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
      721 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
      781 tcgttgaccg aatcaccgac ctctctcccc agggggatcc accggagctt accatgaccg
      841 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
      901 tcgccgccgc gttcgncgac taccccgcca cgcgccacac cgtcgatccg gaccgcncat
      961 cgagcgggtc accgagctgc aagaactctt cncacgnncg tcgggctcga cntcggnaag
     1021 gtnnnngnng nggnnnnngn c
//
