LOCUS       sequence-32493-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-1933
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(443..467)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             493..534
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(574..593)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttg
      121 gcttctaaat tacctacctt ccttcctttt ctttctttca ctcattcctt ggcagtttca
      181 tacatgtgta agatgcattc tggttacccc tactcctctc agcacctcag catatctgtc
      241 ctgatcccac agtcatggtg ttcggtctta gtttgtgacc agtttagttt aaccaggacc
      301 atcagtgtga ctattggatt gggactatcc actgcggtga gtgtggtgcg gtcactggtt
      361 aggtatactg ctgaaggtaa tgactgtgtt tcccagaatc tttcagtagg aaacagttta
      421 gcagagaaga ttagacctac ccacccagct ttcttgtaca aagtggttga tctagagggc
      481 ccgcggttcg aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc
      541 ggttagtaat gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga
      601 aggaacccgc gctatgacgg cataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt
      661 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
      721 ccatgggggc caatacgccc cgcgtttctt ccttttcccc accccacccc cccaagtttc
      781 gggtgaaagg
//
