LOCUS       sequence-324932-       13134 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression construct encoding a TagBFP in frame with a 
            self-cleaving wild-type RLR sensor RIG-I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13134)
  AUTHORS   Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
  TITLE     Nuclear sensing of breaks in mitochondrial DNA enhances immune 
            surveillance.
  JOURNAL   Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w.
            Epub 2021 Feb 24.
  PUBMED    33627873
REFERENCE   2  (bases 1 to 13134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03269-w"; journalName: "Nature"; date: 
            "2021-03"; volume: "591"; issue: "7850"; pages: "477-481"
COMMENT     Sequence Label: pTRIP-SFFV-mtagBFP-2A RIGI WT
FEATURES             Location/Qualifiers
     source          1..13134
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     LTR             7538..7718
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9246..9265)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9479..9498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9598..9620
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9658..9676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9744..9848
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9849..9917,9918..10709)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10067..10086)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10880..11468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11369..11388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11622..11639
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11709..11729)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11714..12043
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11894..12029
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc catgaccacc
     4501 gagcagcgac gcagcctgca agccttccag gattatatcc ggaagaccct ggaccctacc
     4561 tacatcctga gctacatggc cccctggttt agggaggaag aggtgcagta tattcaggct
     4621 gagaaaaaca acaagggccc aatggaggct gccacacttt ttctcaagtt cctgttggag
     4681 ctccaggagg aaggctggtt ccgtggcttt ttggatgccc tagaccatgc aggttattct
     4741 ggactttatg aagccattga aagttgggat ttcaaaaaaa ttgaaaagtt ggaggagtat
     4801 agattacttt taaaacgttt acaaccagaa tttaaaacca gaattatccc aaccgatatc
     4861 atttctgatc tgtctgaatg tttaattaat caggaatgtg aagaaattct acagatttgc
     4921 tctactaagg ggatgatggc aggtgcagag aaattggtgg aatgccttct cagatcagac
     4981 aaggaaaact ggcccaaaac tttgaaactt gctttggaga aagaaaggaa caagttcagt
     5041 gaactgtgga ttgtagagaa aggtataaaa gatgttgaaa cagaagatct tgaggataag
     5101 atggaaactt ctgacataca gattttctac caagaagatc cagaatgcca gaatcttagt
     5161 gagaattcat gtccaccttc agaagtgtct gatacaaact tgtacagccc atttaaacca
     5221 agaaattacc aattagagct tgctttgcct gctatgaaag gaaaaaacac aataatatgt
     5281 gctcctacag gttgtggaaa aacctttgtt tcactgctta tatgtgaaca tcatcttaaa
     5341 aaattcccac aaggacaaaa ggggaaagtt gtcttttttg cgaatcagat cccagtgtat
     5401 gaacagcaga aatctgtatt ctcaaaatac tttgaaagac atgggtatag agttacaggc
     5461 atttctggag caacagctga gaatgtccca gtggaacaga ttgttgagaa caatgacatc
     5521 atcattttaa ctccacagat tcttgtgaac aaccttaaaa agggaacgat tccatcacta
     5581 tccatcttta ctttgatgat atttgatgaa tgccacaaca ctagtaaaca acacccgtac
     5641 aatatgatca tgtttaatta tctagatcag aaacttggag gatcttcagg cccactgccc
     5701 caggtcattg ggctgactgc ctcggttggt gttggggatg ccaaaaacac agatgaagcc
     5761 ttggattata tctgcaagct gtgtgcttct cttgatgcgt cagtgatagc aacagtcaaa
     5821 cacaatctgg aggaactgga gcaagttgtt tataagcccc agaagttttt caggaaagtg
     5881 gaatcacgga ttagcgacaa atttaaatac atcatagctc agctgatgag ggacacagag
     5941 agtctggcaa agagaatctg caaagacctc gaaaacttat ctcaaattca aaatagggaa
     6001 tttggaacac agaaatatga acaatggatt gttacagttc agaaagcatg catggtgttc
     6061 cagatgccag acaaagatga agagagcagg atttgtaaag ccctgttttt atacacttca
     6121 catttgcgga aatataatga tgccctcatt atcagtgagc atgcacgaat gaaagatgct
     6181 ctggattact tgaaagactt cttcagcaat gtccgagcag caggattcga tgagattgag
     6241 caagatctta ctcagagatt tgaagaaaag ctgcaggaac tagaaagtgt ttccagggac
     6301 cccagcaatg agaatcctaa acttgaagac ctctgcttca tcttacaaga agagtaccac
     6361 ttaaacccag agacaataac aattctcttt gtgaaaacca gagcacttgt ggacgcttta
     6421 aaaaattgga ttgaaggaaa tcctaaactc agttttctaa aacctggcat attgactgga
     6481 cgtggcaaaa caaatcagaa cacaggaatg accctcccgg cacagaagtg tatattggat
     6541 gcattcaaag ccagtggaga tcacaatatt ctgattgcca cctcagttgc tgatgaaggc
     6601 attgacattg cacagtgcaa tcttgtcatc ctttatgagt atgtgggcaa tgtcatcaaa
     6661 atgatccaaa ccagaggcag aggaagagca agaggtagca agtgcttcct tctgactagt
     6721 aatgctggtg taattgaaaa agaacaaata aacatgtaca aagaaaaaat gatgaatgac
     6781 tctattttac gccttcagac atgggacgaa gcagtattta gggaaaagat tctgcatata
     6841 cagactcatg aaaaattcat cagagatagt caagaaaaac caaaacctgt acctgataag
     6901 gaaaataaaa aactgctctg cagaaagtgc aaagccttgg catgttacac agctgacgta
     6961 agagtgatag aggaatgcca ttacactgtg cttggagatg cttttaagga atgctttgtg
     7021 agtagaccac atcccaagcc aaagcagttt tcaagttttg aaaaaagagc aaagatattc
     7081 tgtgcccgac agaactgcag ccatgactgg ggaatccatg tgaagtacaa gacatttgag
     7141 attccagtta taaaaattga aagttttgtg gtggaggata ttgcaactgg agttcagaca
     7201 ctgtactcga agtggaagga ctttcatttt gagaagatac catttgatcc agcagaaatg
     7261 tccaaatgac tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     7321 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     7381 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     7441 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa aatcgtcgag
     7501 agatgctgca tataagcagc tgctttttgc ttgtactggg tctctctggt tagaccagat
     7561 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7621 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     7681 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat
     7741 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc cttgacatta
     7801 taatagattt agcaggaatt gaactaggag tggagcacac aggcaaagct gcagaagtac
     7861 ttggaagaag ccaccagaga tactcacgat tctgcacata cctggctaat cccagatcct
     7921 aaggattaca ttaagtttac taacatttat ataatgattt atagtttaaa gtataaactt
     7981 atctaattta ctattctgac agatattaat taatcctcaa atatcataag agatgattac
     8041 tattatcccc atttaacaca agaggaaact gagagggaaa gatgttgaag taattttccc
     8101 acaattacag catccgttag ttacgactct atgatcttct gacacaaatt ccatttactc
     8161 ctcaccctat gactcagtcg aatatatcaa agttatggac attatgctaa gtaacaaatt
     8221 acccttttat atagtaaata ctgagtagat tgagagaaga aattgtttgc aaacctgaat
     8281 agcttcaaga agaagagaag tgaggataag aataacagtt gtcatttaac aagttttaac
     8341 aagtaacttg gttagaaagg gattcaaatg cataaagcaa gggataaatt tttctggcaa
     8401 caagactata caatataacc ttaaatatga cttcaaataa ttgttggaac ttgataaaac
     8461 taattaaata ttattgaaga ttatcaatat tataaatgta atttactttt aaaaagggaa
     8521 catagaaatg tgtatcatta gagtagaaaa caatccttat tatcacaatt tgtcaaaaca
     8581 agtttgttat taacacaagt agaatactgc attcaattaa gttgactgca gattttgtgt
     8641 tttgttaaaa ttagaaagag ataacaacaa tttgaattat tgaaagtaac atgtaaatag
     8701 ttctacatac gttcttttga catcttgttc aatcattgat cgaagttctt tatcttggaa
     8761 gaatttgttc caaagactct gaaataagga aaacaatcta ttatatagtc tcacaccttt
     8821 gttttacttt tagtgatttc aatttaataa tgtaaatggt taaaatttat tcttctctga
     8881 gatcatttca cattgcagat agaaaacctg agactggggt aatttttatt aaaatctaat
     8941 ttaatctcag aaacacatct ttattctaac atcaattttt ccagtttgat attatcatat
     9001 aaagtcagcc ttcctcatct gcaggttcca caacaaaaat ccaaccaact gtggatcaaa
     9061 aatattggga aaaaattaaa aatagcaata caacaataaa aaaatacaaa tcagaaaaac
     9121 agcacagtat aacaacttta tttagcattt acaatctatt aggtattata agtaatctag
     9181 ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc
     9241 tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat
     9301 gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc
     9361 catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact
     9421 gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta
     9481 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     9541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     9661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     9721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     9841 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     9901 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     9961 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    10021 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    10081 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    10141 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    10201 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    10261 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    10321 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    10381 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    10441 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    10501 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    10561 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    10621 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    10681 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    10741 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10801 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10861 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10921 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10981 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11041 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11101 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11161 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11221 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    11281 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    11341 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    11401 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    11461 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    11521 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    11581 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    11641 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    11701 gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
    11761 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
    11821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
    11881 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
    11941 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
    12001 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg gacacaagac
    12061 aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga ggcagtgcgt
    12121 aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc tataatctcg
    12181 cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct gggacacttc
    12241 acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg
    12301 caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc
    12361 tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
    12421 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
    12481 tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat
    12541 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc
    12601 gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac agccgtggga
    12661 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
    12721 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
    12781 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
    12841 atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt
    12901 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
    12961 tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg
    13021 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt ggcggcaact
    13081 ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg tgtg
//
