LOCUS       sequence-324958-       10886 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression plasmid encoding an APEX2 RIG-I fusion product
            in frame with a self-cleaving eGFP. APEX biotin ligase can 
            biotynilate RNAs in proximity of the RLR RIG-I sensor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Tigano M, Vargas DC, Tremblay-Belzile S, Fu Y, Sfeir A
  TITLE     Nuclear sensing of breaks in mitochondrial DNA enhances immune 
            surveillance.
  JOURNAL   Nature. 2021 Mar;591(7850):477-481. doi: 10.1038/s41586-021-03269-w.
            Epub 2021 Feb 24.
  PUBMED    33627873
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-021-03269-w"; journalName: "Nature"; date: 
            "2021-03"; volume: "591"; issue: "7850"; pages: "477-481"
COMMENT     Sequence Label: pLenti-RIGI-Apex-
            T2A-EGFP
FEATURES             Location/Qualifiers
     source          1..10886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     1717..1737
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    1956..2073
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2142..2353
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2393..3142
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="MGKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAG
                     TFDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVA
                     VEVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGH
                     TIGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVD
                     KYAADEDAFFADYAEAHQKLSELGFADA"
     CDS             5951..6007
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(6008..6010,6011..6013,6014..6724)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6053..6074)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6314..6333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6661..6682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6743..7331
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6796..6816)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7214..7225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7403..7636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7714..7835
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7751..7770)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7805..7824
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7875..8010
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8030..8049)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8031..8049)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8059..8076)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8059..8075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8068..8090)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8217..8672
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8304..8323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8514..8535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8698..8802
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8803..8871,8872..9663)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9021..9040)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9834..10422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10323..10342
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10576..10593
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10710..10731
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10746..10751,10752..10769,10770..10776)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10784..10800
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10789..10811
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10808..10824
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10808..10824
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10843..10863
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10845..10863
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
     1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
     1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     1861 gcagttttaa aattatgttt taaaatggac tatcatacat tccatcgatg tagtcaattc
     1921 tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     1981 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     2041 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     2101 ccactttggc gccggctcga gtggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     2161 cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg
     2221 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     2281 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     2341 cgccagaaca caggtgtcgt gacgcgggat cctgtacata cgtaggccgg ccatgggaaa
     2401 gtcttaccca actgtgagtg ctgattacca ggacgccgtt gagaaggcga agaagaagct
     2461 cagaggcttc atcgctgaga agagatgcgc tcctctaatg ctccgtttgg cattccactc
     2521 tgctggaacc tttgacaagg gcacgaagac cggtggaccc ttcggaacca tcaagcaccc
     2581 tgccgaactg gctcacagcg ctaacaacgg tcttgacatc gctgttaggc ttttggagcc
     2641 actcaaggcg gagttcccta ttttgagcta cgccgatttc taccagttgg ctggcgttgt
     2701 tgccgttgag gtcacgggtg gacctaaggt tccattccac cctggaagag aggacaagcc
     2761 tgagccacca ccagagggtc gcttgcccga tcccactaag ggttctgacc atttgagaga
     2821 tgtgtttggc aaagctatgg ggcttactga ccaagatatc gttgctctat ctgggggtca
     2881 cactattgga gctgcacaca aggagcgttc tggatttgag ggtccctgga cctctaatcc
     2941 tcttattttc gacaactcat acttcacgga gttgttgagt ggtgagaagg aaggtctcct
     3001 tcagctacct tctgacaagg ctcttttgtc tgaccctgta ttccgccctc tcgttgacaa
     3061 atatgcagcg gacgaagatg ccttctttgc tgattacgct gaggctcacc aaaagctttc
     3121 cgagcttggg tttgctgatg ccggatccat gaccaccgag cagcgacgca gcctgcaagc
     3181 cttccaggat tatatccgga agaccctgga ccctacctac atcctgagct acatggcccc
     3241 ctggtttagg gaggaagagg tgcagtatat tcaggctgag aaaaacaaca agggcccaat
     3301 ggaggctgcc acactttttc tcaagttcct gttggagctc caggaggaag gctggttccg
     3361 tggctttttg gatgccctag accatgcagg ttattctgga ctttatgaag ccattgaaag
     3421 ttgggatttc aaaaaaattg aaaagttgga ggagtataga ttacttttaa aacgtttaca
     3481 accagaattt aaaaccagaa ttatcccaac cgatatcatt tctgatctgt ctgaatgttt
     3541 aattaatcag gaatgtgaag aaattctaca gatttgctct actaagggga tgatggcagg
     3601 tgcagagaaa ttggtggaat gccttctcag atcagacaag gaaaactggc ccaaaacttt
     3661 gaaacttgct ttggagaaag aaaggaacaa gttcagtgaa ctgtggattg tagagaaagg
     3721 tataaaagat gttgaaacag aagatcttga ggataagatg gaaacttctg acatacagat
     3781 tttctaccaa gaagatccag aatgccagaa tcttagtgag aattcatgtc caccttcaga
     3841 agtgtctgat acaaacttgt acagcccatt taaaccaaga aattaccaat tagagcttgc
     3901 tttgcctgct atgaaaggaa aaaacacaat aatatgtgct cctacaggtt gtggaaaaac
     3961 ctttgtttca ctgcttatat gtgaacatca tcttaaaaaa ttcccacaag gacaaaaggg
     4021 gaaagttgtc ttttttgcga atcagatccc agtgtatgaa cagcagaaat ctgtattctc
     4081 aaaatacttt gaaagacatg ggtatagagt tacaggcatt tctggagcaa cagctgagaa
     4141 tgtcccagtg gaacagattg ttgagaacaa tgacatcatc attttaactc cacagattct
     4201 tgtgaacaac cttaaaaagg gaacgattcc atcactatcc atctttactt tgatgatatt
     4261 tgatgaatgc cacaacacta gtaaacaaca cccgtacaat atgatcatgt ttaattatct
     4321 agatcagaaa cttggaggat cttcaggccc actgccccag gtcattgggc tgactgcctc
     4381 ggttggtgtt ggggatgcca aaaacacaga tgaagccttg gattatatct gcaagctgtg
     4441 tgcttctctt gatgcgtcag tgatagcaac agtcaaacac aatctggagg aactggagca
     4501 agttgtttat aagccccaga agtttttcag gaaagtggaa tcacggatta gcgacaaatt
     4561 taaatacatc atagctcagc tgatgaggga cacagagagt ctggcaaaga gaatctgcaa
     4621 agacctcgaa aacttatctc aaattcaaaa tagggaattt ggaacacaga aatatgaaca
     4681 atggattgtt acagttcaga aagcatgcat ggtgttccag atgccagaca aagatgaaga
     4741 gagcaggatt tgtaaagccc tgtttttata cacttcacat ttgcggaaat ataatgatgc
     4801 cctcattatc agtgagcatg cacgaatgaa agatgctctg gattacttga aagacttctt
     4861 cagcaatgtc cgagcagcag gattcgatga gattgagcaa gatcttactc agagatttga
     4921 agaaaagctg caggaactag aaagtgtttc cagggacccc agcaatgaga atcctaaact
     4981 tgaagacctc tgcttcatct tacaagaaga gtaccactta aacccagaga caataacaat
     5041 tctctttgtg aaaaccagag cacttgtgga cgctttaaaa aattggattg aaggaaatcc
     5101 taaactcagt tttctaaaac ctggcatatt gactggacgt ggcaaaacaa atcagaacac
     5161 aggaatgacc ctcccggcac agaagtgtat attggatgca ttcaaagcca gtggagatca
     5221 caatattctg attgccacct cagttgctga tgaaggcatt gacattgcac agtgcaatct
     5281 tgtcatcctt tatgagtatg tgggcaatgt catcaaaatg atccaaacca gaggcagagg
     5341 aagagcaaga ggtagcaagt gcttccttct gactagtaat gctggtgtaa ttgaaaaaga
     5401 acaaataaac atgtacaaag aaaaaatgat gaatgactct attttacgcc ttcagacatg
     5461 ggacgaagca gtatttaggg aaaagattct gcatatacag actcatgaaa aattcatcag
     5521 agatagtcaa gaaaaaccaa aacctgtacc tgataaggaa aataaaaaac tgctctgcag
     5581 aaagtgcaaa gccttggcat gttacacagc tgacgtaaga gtgatagagg aatgccatta
     5641 cactgtgctt ggagatgctt ttaaggaatg ctttgtgagt agaccacatc ccaagccaaa
     5701 gcagttttca agttttgaaa aaagagcaaa gatattctgt gcccgacaga actgcagcca
     5761 tgactgggga atccatgtga agtacaagac atttgagatt ccagttataa aaattgaaag
     5821 ttttgtggtg gaggatattg caactggagt tcagacactg tactcgaagt ggaaggactt
     5881 tcattttgag aagataccat ttgatccagc agaaatgtcc aaacctgcag gtcgagctag
     5941 cggcagcggc gccaccaact tcagcctgct gaagcaggcc ggcgacgtgg aggagaaccc
     6001 cggccccatg gtgtctaagg gcgaagagct gttcaccggg gtggtgccca tcctggtcga
     6061 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     6121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     6181 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     6241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     6301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     6361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     6421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     6481 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     6541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6661 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6721 caagtaaacg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     6781 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6841 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6901 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6961 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7021 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7081 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7141 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7201 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7261 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7321 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     7381 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     7441 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7501 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7561 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7621 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     7681 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     7741 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7801 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     7861 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     7921 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     7981 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8041 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8101 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8161 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8221 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8281 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8341 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8401 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8461 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     8521 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     8581 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     8641 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     8701 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     8761 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     8821 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     8881 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     8941 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9001 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9061 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9121 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9181 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9241 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9301 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9361 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9421 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     9481 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     9541 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     9601 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     9661 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     9721 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     9781 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     9841 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     9901 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     9961 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
    10021 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    10081 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    10141 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    10201 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    10261 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    10321 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    10381 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    10441 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    10501 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    10561 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
    10621 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
    10681 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
    10741 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
    10801 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
    10861 agggaacaaa agctggagct gcaagc
//
