LOCUS       sequence-32496-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-1964
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(449..473)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             499..540
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(580..599)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    624..672
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttg
      121 atcaagaaag gcacctcagc tagtaataac caggcttact caactttcaa aaatgaacct
      181 ttctgaactt taaattcttg cccaaaattt tctacatcaa agcaactaac acaaacatct
      241 taacctttct tgcgaagctg gagcacaaaa gccggtgccg gtgtgatgcc tacaccgact
      301 tctgggctcc ggctttggaa ggaaggaggt agcctcggct ggcattgctg gtattgcctt
      361 gactaatcct cacacctact tgcagaggta tgctagtacc acagggagga ggggacagat
      421 gccaaaggga gcagggagtc aactgtccac ccagctttct tgtacaaagt ggttgatcta
      481 gagggcccgc ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg
      541 cgtaccggtt agtaatgagt ttaaacgggg gaggctaact gaaacacgga aggagacaat
      601 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acgggtgttg
      661 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac
      721 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa
      781 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcag
      841 atctgcgcag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
      901 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgc
//
