LOCUS       sequence-32498-e         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-1839
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    94..118
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(476..500)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             526..567
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(607..626)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    651..699
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggcttt
      121 gaacccttga tcttcctgac tctaccttct aagttctggg attccaggca tgctcagtta
      181 aaacaatagg tggatttagt catattcttc tggagactaa gacaatagag tccttgaagt
      241 caagctgact ctgcctttag cctcctacat gaaaaggtag atagaacagg tcttgtttgc
      301 aaaataaatt caagacctac ttatctacca acagccattg cctacgtccc acagtcttcc
      361 tacaagaagt gggtgcagtg aactcctaga tgaggaaggc tgggaaagcc agagtggaca
      421 cagtctctac tagtgataga aaggtgcttg gaggtcagac aggcgggtta gaaagaccca
      481 gctttcttgt acaaagtggt tgatctagag ggcccgcggt tcgaaggtaa gcctatccct
      541 aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgagttta aacgggggag
      601 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
      661 aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
      721 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
      781 cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg
      841 tcggggcggc aggccctgcc atagca
//
