LOCUS       sequence-324995-       10427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the ZEM transcription factor under the translational 
            control of the yeast GCN4 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Muller R, Meacham ZA, Ferguson L, Ingolia NT
  TITLE     CiBER-seq dissects genetic networks by quantitative CRISPRi 
            profiling of expression phenotypes.
  JOURNAL   Science. 2020 Dec 11;370(6522). pii: 370/6522/eabb9662. doi: 
            10.1126/science.abb9662.
  PUBMED    33303588
REFERENCE   2  (bases 1 to 10427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-12-11"; volume: "370(6522). pii: 370/6522/eabb9662. 
            doi"; pages: " 10.1126/science.abb9662"
COMMENT     Sequence Label: pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
FEATURES             Location/Qualifiers
     source          1..10427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      65..254
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             1894..2829
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1942..2832
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     protein_bind    4332..4365
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     gene            4420..5995
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        4420..4763
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             4764..5792
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             4764..5792
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      5798..5995
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     protein_bind    6058..6091
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6134..6153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6135..6153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6163..6179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6172..6194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(6958..6991)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(7461..7477)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7576..7593)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7747..8335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7827..7846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8457..9248,9249..9317))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9080..9099
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9318..9409)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9451..9470
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9476..9495
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    9702..9735
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     terminator      complement(join(10268..10427,1..6))
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     10354..10378
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 attcgcttat ttagaagtgt caacaacgta tctaccaacg gaatgcgtgc gatcgcgtgc
       61 attcatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
      121 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
      181 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
      241 tgggacgctc gaagatcgcg tcagctgaag gacttcaact caagacgcac agatattata
      301 acatctgcat aataggcatt tgcaagaatt actcgtgagt aaggaaagag tgaggaacta
      361 tcgcatacct gcatttaaag atgccgattt gggcgcgaat cctttatttt ggcttcaccc
      421 tcatactatt atcagggcca gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac
      481 cctcataaag cacgtggcct cttatcgaga aagaaattac cgtcgctcgt gatttgtttg
      541 caaaaagaac aaaactgaaa aaacccagac acgctcgact tcctgtcttc ctattgattg
      601 cagcttccaa tttcgtcaca caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag
      661 caatcgaagg ttctggaatg gcgggaaagg gtttagtacc acatgctatg atgcccactg
      721 tgatctccag agcaaagttc gttcgatcgt actgttactc tctctctttc aaacagaatt
      781 gtccgaatcg tgtgacaaca acagcctgtt ctcacacact cttttcttct aaccaagggg
      841 gtggtttagt ttagtagaac ctcgtgaaac ttacatttac atatatataa acttgcataa
      901 attggtcaat gcaagaaata catatttggt cttttctaat tcgtagtttt tcaagttctt
      961 agatgctttc tttttctctt ttttacagat catcaaggaa gtaattatct actttttaca
     1021 acaaataaac aaaacaaaac aaaaactcac aacacaggtt actctccccc ctaaattcaa
     1081 attttttttg cccatcagtt tcactagcga attatacaac tcaccagcca cacagctcac
     1141 tcatctactt cgcaatcaaa acaaaatatt ttattttagt tcagtttatt aagttattat
     1201 cagtatcgta ttaaaaaatt aaagatcatt gaaaaatggc ttgctaaacc gattatattt
     1261 tgtttttaaa gtagattatt attagaaaat tattaagaga attatgtgtt aaatttattg
     1321 aaagagaaaa tttattttcc cttattaatt aaagtccttt actttttttg aaaactgtca
     1381 gttttttgaa gagttatttg ttttgttacc aattgctatc atgtacccgt agaattttat
     1441 tcaagatgtt tccgtaacgg ttacctttct gtcaaattat ccaggtttac tcgccaataa
     1501 aaatttccct atactatcat taattaaatc attattatta ctaaagtttt gtttaccaat
     1561 ttgtctgctc aagaaaataa attaaataca aataaaatgg gtacccgccc atatgcttgc
     1621 cctgtcgagt cctgcgatcg ccgcttttct cgctcggatg agcttacccg ccatatccgc
     1681 atccataccg gtcagaagcc cttccagtgt cgaatctgca tgcgtaactt cagtcgtagt
     1741 gaccacctta ccacccacat ccgcacccac acaggcgaga agccttttgc ctgtgacatt
     1801 tgtgggagga agtttgccag gagtgatgaa cgcaagaggc ataccaaaat ccatacaggt
     1861 agatccggtg acggtgctgg tttaattaac tctgctggag acatgagagc tgccaacctt
     1921 tggccaagcc cgctcatgat caaacgctct aagaagaaca gcctggcctt gtccctgacg
     1981 gccgaccaga tggtcagtgc cttgttggat gctgagcccc ccatactcta ttccgagtat
     2041 gatcctacca gacccttcag tgaagcttcg atgatgggct tactgaccaa cctggcagac
     2101 agggagctgg ttcacatgat caactgggcg aagagggtgc caggctttgt ggatttgacc
     2161 ctccatgatc aggtccacct tctagaatgt gcctggctag agatcctgat gattggtctc
     2221 gtctggcgct ccatggagca cccagtgaag ctactgtttg ctcctaactt gctcttggac
     2281 aggaaccagg gaaaatgtgt agagggcatg gtggagatct tcgacatgct gctggctaca
     2341 tcatctcggt tccgcatgat gaatctgcag ggagaggagt ttgtgtgcct caaatctatt
     2401 attttgctta attctggagt gtacacattt ctgtccagca ccctgaagtc tctggaagag
     2461 aaggaccata tccaccgagt cctggacaag atcacagaca ctttgatcca cctgatggcc
     2521 aaggcaggcc tgaccctgca gcagcagcac cagcggctgg cccagctcct cctcatcctc
     2581 tcccacatca ggcacatgag taacaaaggc atggagcatc tgtacagcat gaagtgcaag
     2641 aacgtggtgc ccctctatga cctgctgctg gagatgctgg acgcccaccg cctacatgcg
     2701 cccactagcc gtggaggggc atccgtggag gagacggacc aaagccactt ggccactgcg
     2761 ggctctactt catcgcattc cttgcaaaag tattacatca cgggggaggc agagggtttc
     2821 cctgccacag tcgccgcagc aggtgacggt gctggtttaa ttaacatgac ggtcgaccat
     2881 gatttcaata gcgaagatat tttattcccc atagaaagca tgagtagtat acaatacgtg
     2941 gagaataata acccaaataa tattaacaac gatgttatcc cgtattctct agatatcaaa
     3001 aacactgtct tagatagtgc ggatctcaat gacattcaaa atcaagaaac ttcactgaat
     3061 ttggggcttc ctccactatc tttcgactct ccactgcccg taacggaaac gataccatcc
     3121 actaccgata acagcttgca tttgaaagct gatagcaaca aaaatcgcga tgcaagaact
     3181 attgaaaatg atagtgaaat taagagtact aataatgcta gtggctctgg ggcaaatcaa
     3241 tacacaactc ttacttcacc ttatcctatg aacgacattt tgtacaacat gaacaatccg
     3301 ttacaatcac cgtcaccttc atcggtacct caaaatccga ctataaatcc tcccataaat
     3361 acagcaagta acgaaactaa tttatcgcct caaacttcaa atggtaatga aactcttata
     3421 tctcctcgag cccaacaaca tacgtccatt aaagataatc gtctgtcctt acctaatggt
     3481 gctaattcga atcttttcat tgacactaac ccaaacaatt tgaacgaaaa actaagaaat
     3541 caattgaact cagatacaaa ttcatattct aactccattt ctaattcaaa ctccaattct
     3601 acgggtaatt taaattccag ttattttaat tcactgaaca tagactccat gctagatgat
     3661 tacgtttcta gtgatctctt attgaatgat gatgatgatg acactaattt atcacgccga
     3721 agatttagcg acgttataac aaaccaagag ctctaagcaa atagctaaat tatatacgaa
     3781 ttaatattat gattaagtgt ttacgtgagt gcgatatttt tattactatc ttatacagtt
     3841 gtatatactc tataaaatga gttgtctatt aattaacgcg atgaatgctt tctgggttta
     3901 cctctccaac aactctagtt tacttctcaa tacattcaat tgtatttgat ttgtcaatac
     3961 ttcatcatta atcaattcta tagttttgtt tttctcgttt atttccaaat ttaatgcatc
     4021 aattttatta ttcaatttgt cgttgatttt ggttaatgat tttatggttt gatctctggc
     4081 attgattgtt tgtgttagtt tttcattatt gataattaaa ttatttaagt tagttatcaa
     4141 ctcggtgttt tcaagtttca agttttcaat ttctttagag tttattagat ttgtcaaagt
     4201 ttctgaattg cttgattggt ccggcccagg tattgttagc ggtttgaagc aggcggcaga
     4261 agaagtaaca aaggaaccta gaggcctttt gatgtggatc cttcgtacgc tgcaggtcga
     4321 caacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt ctagagatct
     4381 gtttagcttg cctcgtcccc gccgggtcac ccggccagcg acatggaggc ccagaatacc
     4441 ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg cccgtacatt
     4501 tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg ccgcacggcg
     4561 cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc tcccctcaca
     4621 gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg ttaggatttg
     4681 ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata cagttctcac
     4741 atcacatccg aacataaaca accatgggta aaaagcctga actcaccgcg acgtctgtcg
     4801 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     4861 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     4921 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     4981 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     5041 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     5101 tgcagccggt cgcggaggca atggatgcca ttgctgcggc cgatcttagc cagacgagcg
     5161 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     5221 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     5281 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     5341 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     5401 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     5461 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     5521 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     5581 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     5641 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     5701 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     5761 gccccagcac tcgtccgagg gcaaaggaat aatcagtact gacaataaaa agattcttgt
     5821 tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat tttaatcaaa
     5881 tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg cgaagttaag
     5941 tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta tactgctgtc
     6001 gattcgatac taacgccgcc atccagtgtc gaaaacgagc tctcgagaac ccttaatata
     6061 acttcgtata atgtatgcta tacgaagtta ttaggtgata tcagatccac tagtggccta
     6121 tgcacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     6181 acgtcgtgac tgggaaaacc ctggcgttac ccctgcagga ctagtgctga ggcattaatg
     6241 caactcagaa gtttgacagc aagcaagttc atcattcgaa ctagccttat tgttttagtt
     6301 cagtgacagc gaactgccgt actcgatgct ttatttctca cggtagagcg gaagaacaga
     6361 taggggcagc gtgagaagag ttagaaagta aatttttatc acgtctgaag tattcttatt
     6421 cataggaaat tttgcaaggt tttttagctc aataacgggc taagttatat aaggtgttca
     6481 cgcgattttc ttgttatgta tacctcttct ctgaggaatg gtactactgt cctgatgtag
     6541 gctccttaaa ttggtgggca agaataactt atcgatattt tgtatattgg tcttggagtt
     6601 caccacgtaa tgcctgttta agaccatcag ttaactctag tattatttgg tcttggctac
     6661 tggccgtttg ctattattca agtcttttgt gccttcccgt cgggtaaggg agttatttag
     6721 ggatacagaa tctaacgaaa actaaatctc aatgattaac tctatttaat ccttttttga
     6781 aaggcaaaag aggtcccttg ttcacttaca acgttcttag ccaaattcgc ttatcactta
     6841 ctacttcacg atatacagaa gtaaaaacat ataaaaagat gtctgtttgt ttagccatca
     6901 caaaaggtat cgcagattaa gacctcagcg cggccgcaaa tttaaataaa atgaagtgaa
     6961 gttcctatac tttctagaga ataggaactt ctatagtgag tcgaataagg gcgacacaaa
     7021 atttattcta aatgcataat aaatactgat aacatcttat agtttgtatt atattttgta
     7081 ttatcgttga catgtataat tttgatatca aaaactgatt ttccctttat tattttcgag
     7141 atttattttc ttaattctct ttaacaaact agaaatattg tatatacaaa aaatcataaa
     7201 taatagatga atagtttaat tataggtgtt catcaatcga aaaagcaacg tatcttattt
     7261 aaagtgcgtt gcttttttct catttataag gttaaataat tctcatatat caagcaaagt
     7321 gacaggcgcc cttaaatatt ctgacaaatg ctctttccct aaactccccc cataaaaaaa
     7381 cccgccgaag cgggttttta cgttatttgc ggattaacga ttactcgtta tcagaaccgc
     7441 ccagggggcc cgagcttaag actggccgtc gttttacaac acagaaagag tttgtagaaa
     7501 cgcaaaaagg ccatccgtca ggggccttct gcttagtttg atgcctggca gttccctact
     7561 ctcgccttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8041 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8161 gtgggctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gacgcgcgcg taactcacgt
     8401 taagggattt tggtcatgag cttgcgccgt cccgtcaagt cagcgtaatg ctctgcttac
     8461 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     8521 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagc
     8581 gctgcgatga taccgcgaga accacgctca ccggctccgg atttatcagc aataaaccag
     8641 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     8701 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     8761 gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     8821 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     8881 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     8941 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9001 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9061 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9121 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9181 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9241 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9301 aaatgttgaa tactcatatt cttccttttt caatattatt gaagcattta tcagggttat
     9361 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggtcagt
     9421 gttacaacca attaaccaat tctgaacatt atcgcgagcc catttatacc tgaatatggc
     9481 tcataacacc ccttgtttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
     9541 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggactcccc atgcgagagt
     9601 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgcc
     9661 cgggctaatt atggggtgtc gcccttattc gactctatag tgaagttcct attctctaga
     9721 aagtatagga acttctgaag tggggattta aatgcggccg cgctgagggt ttaatcaatc
     9781 tggcggcttg agttctcaac atgttttatt ttttacttat attgctggta gggtaaaaaa
     9841 atataactcc taggaatagg ttgtctatat gtttttgtct tgcttctata attgtaacaa
     9901 acaaggaaag ggaaaatact gggtgtaaaa gccattgagt caagttaggt catccctttt
     9961 atacaaaatt tttcaatttt ttttccaaga ttcttgtacg attaattatt ttttttttgc
    10021 gtcctacagc gtgatgaaaa tttcgcctgc tgcaagatga gcgggaacgg gcgaaatgtg
    10081 cacgcgcaca acttacgaaa cgcggatgag tcactgacag ccaccgcaga ggttctgact
    10141 cctactgagc tctattggag gtggcagaac cggtaccgga ggaggccgct ataaccggtt
    10201 tgaatttatt gtcacagtgt cacatcagca ttaagtcctc agcgagctcg catggaatgc
    10261 gtgcgatgag cgacctcatg ctatacctga gaaagcaacc tgacctacag gaaagagtta
    10321 ctcaagaata agaattttcg ttttaaaacc taagagtcac tttaaaattt gtatacactt
    10381 atttttttta taacttattt aataataaaa atcataaatc ataagaa
//
