LOCUS       sequence-32500-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     pMOTag2T
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag2H
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        39..57
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             90..170
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(526..545)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa
       61 caaaagctgg taccgggccc cccctcgagt acccttacga tgtgcctgat tacgcgtacc
      121 catacgacgt gccagactac gcatacccgt acgatgtgcc cgattacgca tagtcgacaa
      181 agtgtgacaa cgtcgcacca tgtgtaggtt ttcatttatg ttctttcttt ctttcttttg
      241 tgaatttgtt ttctgtctca aatgttttta attcgcttgg gacctatgtt tttcttgttt
      301 ttttgctcac cctttgtgta ggaggcaccc tgtcacgtct gtggttgcgt gtatgccttc
      361 cttcccctta ttcgcttctt cctgtcgtgt cacacctctt tctccctctc cctttccgcc
      421 ttttctttca atcttgtttt ctcgaccagc cctactagag gagaaagaat agtaaccctt
      481 tcatcaaaga aaatagttca aacgaattgc tagcttaagc ttaccatgac cgagtacaag
      541 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
      601 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cagaccgcca catcgagcgg
      661 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
      721 gtcgcggacg acggcgcagc agtggcggtc tggaccacgc cggagagcgt cgaagcgggg
      781 gcggtgttcg ccgagatcgg cccgcgcatg gccga
//
