LOCUS       sequence-325006-       11251 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under 
            tetracycline-inducible promoter. SunTag and Renilla luciferase are 
            in frame with the main start codon of ATF4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Mateju D, Eichenberger B, Voigt F, Eglinger J, Roth G, Chao JA
  TITLE     Single-Molecule Imaging Reveals Translation of mRNAs Localized to 
            Stress Granules.
  JOURNAL   Cell. 2020 Dec 23;183(7):1801-1812.e13. doi: 
            10.1016/j.cell.2020.11.010. Epub 2020 Dec 11.
  PUBMED    33308477
REFERENCE   2  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.11.010"; journalName: "Cell"; date: 
            "2020-12-23- 23"; volume: "183"; issue: "7"; pages: "1801-1812.e13"
COMMENT     Sequence Label: pSF4 TetCMV
            ATF4(5'UTR)-SunTag-Renilla-FKBP-St...
FEATURES             Location/Qualifiers
     source          1..11251
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(975..1022)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    1069..1087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1105..1123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1141..1159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1213..1231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1249..1267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1285..1303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1348..1372
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1458..1467
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1780..1836
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1852..1908
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1924..1980
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             1996..2052
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2068..2124
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2140..2196
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2212..2268
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2284..2340
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2356..2412
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2428..2484
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2500..2556
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2572..2628
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2644..2700
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2716..2772
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2788..2844
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2860..2916
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             2932..2988
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3004..3060
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3076..3132
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3148..3204
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3220..3276
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3292..3348
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3436..3492
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             3526..4455
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             join(4456..4458,4459..4779)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             4459..4782
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD-C
                     /note="FKBP12 mutant optimized for use as a C-terminal tag"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KPE"
     polyA_signal    6312..6446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     6390..6409
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    complement(6554..6587)
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     primer_bind     complement(6640..6656)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(6672..6694)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    7105..7462
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     LTR             7527..7996
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(8526..8542)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8526..8542)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8539..8561)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8550..8566
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8574..8580,8581..8598,8599..8604))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8619..8640
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8757..8774)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8928..9516)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9008..9027)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9687..10478,10479..10547))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10310..10329
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10548..10652)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10720..10738
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10776..10798)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10898..10917
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11111..11133
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11125..11142
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11126..11142
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acacacacac acacacacac acacacacac acaatcatac acataaacac aatnncacac
       61 acacacacat gcacaatcac acacacacaa tcacacacaa acatacatac acacaatcac
      121 acacaaccat acacacatat acacaatcac acacacaaac acaatcacac acacacacat
      181 caatcaatca atcaatcaat ataccaaaaa tgatagctca tacctgtaat cttagatgtg
      241 ggaaggctaa aacagcaggc tacccatgac ttcaaggtca ccctgggtta cagagtagct
      301 aatagtctag cttgaactat agtgtaggat ctttaaaaaa aaattaacca accaaccaac
      361 aaacaaacaa acaaacaaca acaacaacaa atggaagaca gaggacaaaa aaaaataaaa
      421 gaaaaagtag aaagccaatg gagatactat cttaaaatta gaaatattta tgacacattc
      481 tgaatactcc atgtttattt caaggaagcc aatgtaactt taagtactat ggccatttaa
      541 tgtaaatact taagaaaaaa aaccaaatta attttgatac atgctgcatg tgaaagaccc
      601 ccgctgacgg gtagtcaatc actcagagga gaccctctca aggaacagcg agaccacaag
      661 tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac tcagtcaatc
      721 ggaggactgg cgccccgagt gaggggttgt gggctcgagc tcggggagca gaagcgcgcg
      781 aacagaagtc gagctagctt gccaaaccta caggtggggt ctttcattcc cccctttttc
      841 tggagactaa ataaaatctt ttattttatc tatggctcgt actctatagg cttcagctgg
      901 tgatattgtt gagtcaaaac tagagcctgg accactgata tcctgtcttt aacaaattgg
      961 actaatccgg atctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     1021 tcgcggccag atcttttgcg gccgctttgg gccggccgaa ttctttactc cctatcagtg
     1081 atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac
     1141 tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta
     1201 tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg
     1261 atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtata agctttaggc
     1321 gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga tcgcctggat
     1381 ttctactttg cccgcccaca gatgtagttt tctctgcgcg tgtgcgtttt ccctcctccc
     1441 cgccctcagg gtccacggcc accatggcgt attaggggca gcagtgcctg cggcagcatt
     1501 ggcctttgca gcggcggcag cagcaccagg ctctgcagcg gcaaccccca gcggcttaag
     1561 ccatggcgct tctcacggca ttcagcagca gcgttgctgt aaccgacaaa gacaccttcg
     1621 aattaagcac attcctcgat tccagcaaag caccgcaaca tgaccgaaat gagcttcctg
     1681 agcagcgagg tgttggtggg ggacttgatg tcccccttcg accagtcggg tttgggggct
     1741 gacggtccgg gtggatctgg aggtggaggt tctggaggag aagaactttt gagcaagaat
     1801 tatcatcttg agaacgaagt ggctcgtctt aagaaaggtt ctggcagtgg agaagaactg
     1861 ctttcaaaga attaccacct ggaaaatgag gtagctagac tgaaaaaggg gagcggaagt
     1921 ggggaggagt tgctgagcaa aaattatcat ttggagaacg aagtagcacg actaaagaaa
     1981 gggtccggat cgggtgagga gttactctcg aaaaattatc atctcgaaaa cgaagtggct
     2041 cggctaaaaa agggcagtgg ttctggagaa gagctattat ctaaaaacta ccacctcgaa
     2101 aatgaggtgg cacgcttaaa aaagggaagt ggcagtggtg aagagctact atccaagaat
     2161 tatcatcttg agaacgaggt agcgcgtttg aagaagggtt ccggctcagg agaggaactg
     2221 ctctcgaaga actatcatct tgaaaatgag gtcgctcgat taaaaaaggg atcgggcagt
     2281 ggtgaggaac tactttcaaa gaattaccac ctcgaaaacg aagtagctcg attaaagaaa
     2341 ggttcagggt cgggtgaaga attactgagt aaaaattatc atctggaaaa tgaggtagcg
     2401 agactaaaaa aggggagtgg ttctggcgaa gagttgctat cgaaaaatta tcatcttgag
     2461 aacgaagttg ctaggctcaa aaagggctca ggctcaggcg aggagttgct ctcgaaaaac
     2521 taccacttgg aaaatgaggt cgcgaggttg aaaaagggga gcgggtcggg cgaggagtta
     2581 ttgagcaaaa actatcattt agagaacgaa gtcgcgcgct taaagaaagg ctcgggctcg
     2641 ggcgaagaac tcttatcgaa gaactaccac ctcgaaaatg aggtcgccag gttgaaaaag
     2701 ggcagtggca gcggggagga actcttgagc aagaactacc acttggagaa tgaggtcgcg
     2761 agattgaaga aagggtcggg gagcggcgag gaattgctca gcaagaatta tcatttggag
     2821 aacgaagtcg ccaggctcaa gaaaggctcg gggtcggggg aggaattgtt gagtaaaaac
     2881 taccacttgg aaaatgaagt cgccaggctc aaaaaaggga gtgggagcgg cgaagagtta
     2941 ttgagcaaaa attaccactt ggagaacgaa gtggcaaggc tcaagaaagg gagcggcagc
     3001 ggggaggagc tcttatcgaa gaactaccac ttagagaatg aagtcgcccg cttgaagaaa
     3061 ggctcgggga gcggggaaga gctcttgagc aagaactacc acttggaaaa tgaggtggcg
     3121 cgcttgaaga aagggagcgg gagcggggaa gagttactat ctaagaatta tcatctcgag
     3181 aacgaggtgg ctcgactaaa gaagggctcc ggcagtgggg aggaactcct gtcgaagaac
     3241 tatcatcttg aaaatgaggt tgcaagactt aaaaaggggt ccggatcagg tgaggaacta
     3301 ctcagtaaga attaccacct ggaaaacgaa gtagctcgat taaagaaagg ttcagggtcg
     3361 ggtgaagaac tgctctcaaa agattatcat ttggaaaatg aggttgcacg tttaaaaaag
     3421 ggaagtggca gtggtgagga acttctgtcg aaaaattatc atctcgagaa tgaagtagcc
     3481 cgacttaaaa agggttctgg ctcgggtcag cggccgcacc ggtcagcttc caaggtgtac
     3541 gaccccgagc aacgcaaacg catgatcact gggcctcagt ggtgggctcg ctgcaagcaa
     3601 atgaacgtgc tggactcctt catcaactac tatgattccg agaagcacgc cgagaacgcc
     3661 gtgatttttc tgcatggtaa cgctgcctcc agctacctgt ggaggcacgt cgtgcctcac
     3721 atcgagcccg tggctagatg catcatccct gatctgatcg gaatgggtaa gtccggcaag
     3781 agcgggaatg gctcatatcg cctcctggat cactacaagt acctcaccgc ttggttcgag
     3841 ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg actggggggc ttgtctggcc
     3901 tttcactact cctacgagca ccaagacaag atcaaggcca tcgtccatgc tgagagtgtc
     3961 gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg aggaggatat cgccctgatc
     4021 aagagcgaag agggcgagaa aatggtgctt gagaataact tcttcgtcga gaccatgctc
     4081 ccaagcaaga tcatgcggaa actggagcct gaggagttcg ctgcctacct ggagccattc
     4141 aaggagaagg gcgaggttag acggcctacc ctctcctggc ctcgcgagat ccctctcgtt
     4201 aagggaggca agcccgacgt cgtccagatt gtccgcaact acaacgccta ccttcgggcc
     4261 agcgacgatc tgcctaagat gttcatcgag tccgaccctg ggttcttttc caacgctatt
     4321 gtcgagggag ctaagaagtt ccctaacacc gagttcgtga aggtgaaggg cctccacttc
     4381 agccaggagg acgctccaga tgaaatgggt aagtacatca agagcttcgt ggagcgcgtg
     4441 ctgaagaacg agcagatggg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc
     4501 ttccccaagc gcggccagac ctgtgtggtg cactacaccg ggatgcttga agatggaaag
     4561 aaagtcgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag
     4621 gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg
     4681 actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat
     4741 gccactctcg tcttcgatgt ggagcttcta aaaccggaat aagggccagg gaagaactgg
     4801 ctcggaaaga ggaccccaca tgttgtagct tcaagtttaa acggatccaa cctacaaacg
     4861 ggtggaggat caccccaccc gacacttcac aatcaagggg tacaatacac aagggtggag
     4921 gaacacccca ccctccagac acattacaca gaaatccaat caaacagaag caccatcagg
     4981 gcttctgcta ccaaatttat ctcaaaaaac tacaacaagg aatcaccatc agggattccc
     5041 tgtgcaatat acgtcaaacg agggccacga cgggaggacg atcacgcctc ccgaatatcg
     5101 gcatgtctgg ctttcgaatt cagtgcgtgg agcatcagcc cacgcagcca atcagagtcg
     5161 aatacaagtc gactttcgcg aagagcatca gccttcgcgc cattcttaca caaaccacac
     5221 tctcccctac aggaacagca tcagcgttcc tgcccagtac ccaactcaag aaaatttatg
     5281 tccccatgca gcatcagcgc atgggcccca agaatacatc cccaacaaaa tcacatccga
     5341 gcaccaacag ggctcggagt gttgtttctt gtccaactgg acaaaccctc catggaccat
     5401 caggccatgg actctcacca acaagacaaa aactactctt ctcgaagcag catcagcgct
     5461 tcgaaacact cgagcataca ttgtgcctat ttcttgggtg gacgatcacg ccacccatgc
     5521 tctcacgaat ttcaaaacac ggacaaggac gagcaccacc agggctcgtc gttccacgtc
     5581 caatacgatt acttaccttt cgggatcacg atcacggatc ccgcagctac atcacttcca
     5641 ctcaggacat tcaagcatgc acgatcacgg catgctccac aagtctcaac cacagaaact
     5701 accaaatggg ttcagcacca gcgaacccac tcctacctca aacctcttcc cacaaaactg
     5761 gcaagcagga tcaccgcttg cccattccaa cataccaaat caaaaacaat tactggtaca
     5821 gcatcagcgt accagcccac atctctcact actatcaaaa accaaaccgt tcagcaacag
     5881 cgaacggtac acacggaaaa atcaactggt ttacaaatac gaaagacgat cacgctttcg
     5941 tccagcgcaa actattacga aaaacatccg acgggaagag caacagcctt cccgcggcgg
     6001 aaaacctcac aaaaacacga caaacggatg cacgaacacg gcatccgccg acaacccaca
     6061 aacttacaac caggcaaacg gtgcaggatc accgcaccgt acatcaaaca cctcagatct
     6121 catttcgcgc gggatccaga ccacctcccc tgcgagctaa gctggacagc caatgacggg
     6181 taagagagtg acatttttca ctaacctaag acaggagggc cgtcagagct actgcctaat
     6241 ccaaagacgg gtaaaagtga taaaaatgta tcactccaac ctaagacagg cgcagcttcc
     6301 gagggatttg agatccagac atgataagat acattgatga gtttggacaa accaaaacta
     6361 gaatgcagtg aaaaaaatgc cttatttgtg aaatttgtga tgctattgcc ttatttgtaa
     6421 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     6481 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg
     6541 ctgattatga tctgaagttc ctatactatt tgaagaatag gaacttcgga ataggaactt
     6601 cggatctcat atggccatat gggggcgcct agagaaggag tgagggctgg ataaagggag
     6661 gatcgaggcg gggtcgaacg aggaggttca agggggagag acggggcgga tggaggaaga
     6721 ggaggcggag gcttagggtg tacaaagggc ttgacccagg gaggggggtc aaaagccaag
     6781 gcttcccagg tcacgatgta ggggacctgg tctgggtgtc catgcgggcc aggtgaaaag
     6841 accttgatct taacctgggt gatgaggtct cggttaaagg tgccgtctcg cggccatccg
     6901 acgttaaagg ttggccattc tgcagagcag aaggtaaccc aacgtctctt cttgacatct
     6961 accgactggt tgtgagcgat ccgctcgaca tctttccagt gacctaaggt caaacttaag
     7021 ggagtggtaa caggctagcc cgggcccata ttttcagaca aatacagaaa cacagtcaga
     7081 cagagacaac acagaacgat gctgcagcag acaagacgcg cggcgcggct tcggtcccaa
     7141 accgaaagca aaaattcaga cggaggcggg aactgtttta ggttctcgtc tcctaccaga
     7201 accacatatc cctcctctaa ggggggtgca ccaaagagtc ctaaacgatc gggattttag
     7261 gactcaggtc gggccacaaa aacggccccc gaagtccctg ggacgtctcc cagggttgcg
     7321 gccgggtgtt ccgaactcgt cagttccacc acgggtccgc cagatacaga gctagttagc
     7381 taactagtac cgacgcaggc gcataaaatc agtcatagac actagacaat cggacagaca
     7441 cagataagtt gctggccagc ttacctcccg gtggtgggtc ggtggtccct gggcaggggt
     7501 ctcccgatcc cggacgagcc cccaaatgaa agacccccgc tgacgggtag tcaatcactc
     7561 agaggagacc ctcccaagga acagcgagac cacaagtcgg atgcaactgc aagagggttt
     7621 attggataca cgggtacccg ggcgactcag tcaatcggag gactggcgcg ccgagtgagg
     7681 ggttgtgggc tcttttattg agctcgggga gcagaagcgc gcgaacagaa gcgagaagcg
     7741 aactgattgg ttagttcaaa taaggcacag ggtcatttca ggtccttggg gcaccctgga
     7801 aacatctgat ggttctctag aaactgctga gggctggacc gcatctgggg accatctgtt
     7861 cttggccctg agccggggca ggaactgctt accacagata tcctgtttgg cccatattca
     7921 gctgttccat ctgttcttgg ccctgagccg gggcaggaac tgcttaccac agatatcctg
     7981 tttggcccat attcagctgt tccatctgtt cctgaccttg atctgaactt ttctattctc
     8041 agttatgtat ttttccatgc cttgcaaaat ggcgttactt aagctagctt gccaaaccta
     8101 caggtggggt ctttcacatg tatatgtcaa aaataaaaat caactaattg actagtaatt
     8161 aatatgactg gcataatggg aaattgatcc tgacagatgc aaactggctt ctcagcagcg
     8221 catttatgtt gtcaactgag gaaggaaacg ttaatgacag aaactctaag taatttccac
     8281 gtttatctat ttttatttat actagctttg gtaacaggaa tattgcagca ttcatgcaca
     8341 ttgaaaccct tatgaaataa aaacatctgt gcatttaaaa tggaattaac attttaaatg
     8401 ttaaaaaaag ctggcttagc ttccccccgc cccctagggc atagaacaag tcaaatgctt
     8461 tatatatttg agtttgggat gtattaggaa actcctaaga gcaaagctag cttggcgtaa
     8521 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     8581 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     8641 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     8701 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     8761 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     8821 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     8881 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     8941 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9001 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9061 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9121 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9181 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9241 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9301 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9361 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9421 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9481 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga tttttgtcat gagattatca
     9601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     9661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     9721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     9781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     9841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     9901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     9961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    10621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
    10681 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
    10741 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    10801 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10861 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    10921 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    10981 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
    11041 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
    11101 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattaat tccaagctaa
    11161 gtgaaagact ctgtctcaaa aaccaacctg acacctgagc cggagcgttg cctctcacta
    11221 ccataaatat atatacacaa cacacacaca c
//
