LOCUS       sequence-325018-       10545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression and localization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Miserey-Lenkei S, Trajkovic K, D'Ambrosio JM, Patel AJ, Copic A, 
            Mathur P, Schauer K, Goud B, Albanese V, Gautier R, Subra M, Kovacs 
            D, Barelli H, Antonny B
  TITLE     A comprehensive library of fluorescent constructs of SARS-CoV-2 
            proteins and their initial characterisation in different cell types.
  JOURNAL   Biol Cell. 2021 Jul;113(7):311-328. doi: 10.1111/boc.202000158. Epub
            2021 May 10.
  PUBMED    33666950
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/boc.202000158"; journalName: "Biol Cell"; date: "2021-07"; 
            volume: "113"; issue: "7"; pages: "311-328"
COMMENT     Sequence Label: Nsp3 -mCherry
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      232..241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6076..6128
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      6130..6139
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6136..6846
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6281..6299)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6549..6568
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6969..7090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7006..7025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7060..7079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7097..7552)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7234..7255)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7446..7465
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        7579..7683
                     /gene="bla"
                     /label=AmpR promoter
     promoter        7685..8042
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7685..7876
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7708..7728)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      7893..8028
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8077..8871
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8077..8871
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8131..8150)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8741..8760
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9059..9078)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9103..9150
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      9479..10067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9968..9987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        10242..10545
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta ggccaccatg
      241 gcccctacca aggtgacctt cggagacgac accgtgatcg aggtgcaggg ttacaagagc
      301 gtgaacatca ccttcgagct ggacgaacgc atcgacaagg tgctgaacga aaagtgctcc
      361 gcttacaccg tggagctggg caccgaggtg aacgaattcg cctgcgtggt ggccgacgct
      421 gtgatcaaga ccctgcagcc agtgtccgaa ctgctgaccc ctctgggtat cgacctggac
      481 gagtggtcta tggctaccta ctacctgttc gacgagagcg gcgaattcaa gctggcctct
      541 cacatgtact gcagcttcta ccctcccgac gaagacgagg aagagggtga ctgcgaagag
      601 gaagagttcg agccttccac tcagtacgag tacggcaccg aagacgacta ccagggcaag
      661 cctctggaat tcggagccac ctctgccgct ctgcagcctg aagaggaaca ggaggaagac
      721 tggctggacg acgactccca gcagaccgtg ggtcagcagg acggctctga agacaaccag
      781 accaccacca tccagaccat cgtggaggtg cagcctcagc tggagatgga actgacccca
      841 gtggtgcaga ccatcgaggt gaacagcttc tccggctacc tgaagctgac cgacaacgtg
      901 tacatcaaga acgctgacat cgtggaggaa gccaagaagg tgaaaccaac cgtggtggtg
      961 aacgccgcta acgtgtacct gaagcacggc ggaggtgtgg ctggagctct gaacaaggct
     1021 accaacaacg ccatgcaggt ggaatccgac gactacatcg ctaccaacgg tcctctgaag
     1081 gtgggcggat cctgcgtgct gtctggccac aacctggcca agcactgcct gcacgtggtg
     1141 ggaccaaacg tgaacaaggg agaggacatc cagctgctga agagcgccta cgaaaacttc
     1201 aaccagcacg aggtgctgct ggctccactg ctgtctgccg gaatcttcgg tgctgatccc
     1261 atccacagcc tgcgcgtgtg cgtggacacc gtgaggacca acgtgtacct ggccgtgttc
     1321 gacaagaacc tgtacgacaa gctggtgagc tccttcctgg agatgaagtc cgaaaagcag
     1381 gtggagcaga agatcgccga gatccccaag gaggaagtga agcctttcat caccgaatct
     1441 aagcccagcg tggagcagag aaagcaggac gacaagaaga tcaaggcctg cgtggaggaa
     1501 gtgaccacca ccctggagga aaccaagttc ctgaccgaga acctgctgct gtacatcgac
     1561 atcaacggca acctgcaccc tgactccgct accctggtgt ctgacatcga catcaccttc
     1621 ctgaagaagg acgcccccta catcgtgggc gacgtggtgc aggaaggagt gctgaccgct
     1681 gtggtcatcc ctaccaagaa ggccggtggc accaccgaga tgctggccaa ggctctgcgt
     1741 aaggtgccaa ccgacaacta catcaccacc taccccggtc agggcctgaa cggttacacc
     1801 gtggaggaag ctaagaccgt gctgaagaag tgcaagtctg ccttctacat cctgccctcc
     1861 atcatctcta acgagaagca ggaaatcctg ggcaccgtga gctggaacct gcgcgaaatg
     1921 ctggcccacg ctgaggaaac caggaagctg atgcccgtgt gcgtggagac caaggccatc
     1981 gtgagcacca tccagagaaa gtacaagggc atcaagatcc aggagggtgt ggtggactac
     2041 ggcgctcgtt tctacttcta cacctccaag accaccgtgg cctctctgat caacaccctg
     2101 aacgacctga acgagaccct ggtgaccatg ccactgggat acgtgaccca cggtctgaac
     2161 ctggaggaag ccgctagata catgcgttcc ctgaaggtgc ctgctaccgt gagcgtgagc
     2221 agccctgacg ctgtgaccgc ctacaacgga tacctgacct cctctagcaa gacccctgag
     2281 gaacacttca tcgaaaccat cagcctggcc ggttcctaca aggactggtc ttacagcgga
     2341 cagtccaccc agctgggcat cgagttcctg aagcgcggag acaagagcgt gtactacacc
     2401 tccaacccaa ccaccttcca cctggacggt gaagtgatca ccttcgacaa cctgaagacc
     2461 ctgctgtctc tgcgcgaggt gaggaccatc aaggtgttca ccaccgtgga caacatcaac
     2521 ctgcacaccc aggtggtgga catgagcatg acctacggac agcagttcgg tcctacctac
     2581 ctggacggag ctgacgtgac caagatcaag ccacacaact cccacgaggg caagaccttc
     2641 tacgtgctgc ctaacgatga caccctgaga gtggaggctt tcgaatacta ccacaccacc
     2701 gacccatcct tcctgggcag gtacatgtct gccctgaacc acaccaagaa gtggaagtac
     2761 ccccaggtga acggactgac cagcatcaag tgggccgaca acaactgcta cctggccacc
     2821 gctctgctga ccctgcagca gatcgaactg aagttcaacc cacccgctct gcaggacgct
     2881 tactacagag cccgtgctgg cgaggccgct aacttctgcg ctctgatcct ggcctactgc
     2941 aacaagaccg tgggcgaact gggagacgtg agagagacca tgagctacct gttccagcac
     3001 gccaacctgg actcctgcaa gcgtgtgctg aacgtggtgt gcaagacctg cggccagcag
     3061 cagaccaccc tgaagggagt ggaagctgtg atgtacatgg gtaccctgtc ctacgagcag
     3121 ttcaagaagg gcgtgcagat cccttgcacc tgcggaaagc aggccaccaa gtacctggtg
     3181 cagcaggaaa gcccattcgt gatgatgtcc gctcctccag cccagtacga actgaagcac
     3241 ggcaccttca cctgcgcttc cgagtacacc ggcaactacc agtgcggaca ctacaagcac
     3301 atcacctcta aggagaccct gtactgcatc gacggagccc tgctgaccaa gtcctctgaa
     3361 tacaagggtc ccatcaccga cgtgttctac aaggagaaca gctacaccac caccatcaag
     3421 cccgtgacct acaagctgga cggcgtggtg tgcaccgaaa tcgaccctaa gctggacaac
     3481 tactacaaga aggacaactc ttacttcacc gagcagccta tcgacctggt gccaaaccag
     3541 ccctacccta acgccagctt cgacaacttc aagttcgtgt gcgacaacat caagttcgct
     3601 gacgacctga accagctgac cggatacaag aagcctgcct ctagagagct gaaggtgacc
     3661 ttcttcccag acctgaacgg cgacgtggtg gctatcgact acaagcacta caccccaagc
     3721 ttcaagaagg gagccaagct gctgcacaag cccatcgtgt ggcacgtgaa caacgctacc
     3781 aacaaggcca cctacaagcc taacacctgg tgcatccgtt gcctgtggtc taccaagcca
     3841 gtggaaacct ccaactcttt cgacgtgctg aagagcgagg acgctcaggg catggacaac
     3901 ctggcctgcg aagacctgaa gcccgtgagc gaggaagtgg tggagaaccc aaccatccag
     3961 aaggacgtgc tggaatgcaa cgtgaagacc accgaggtgg tgggagacat catcctgaag
     4021 ccagctaaca acagcctgaa gatcaccgag gaagtgggtc acaccgacct gatggccgct
     4081 tacgtggaca acagctccct gaccatcaag aagcccaacg agctgagcag ggtgctgggt
     4141 ctgaagaccc tggctactca cggtctggct gctgtgaact ctgtgccatg ggacaccatc
     4201 gctaactacg ccaagccttt cctgaacaag gtggtgtcca ccaccaccaa catcgtgacc
     4261 agatgtctga accgcgtgtg caccaactac atgccctact tcttcaccct gctgctgcag
     4321 ctgtgcacct tcaccagaag caccaactcc cgtatcaagg ctagcatgcc taccaccatc
     4381 gccaagaaca ccgtgaagag cgtgggaaag ttctgcctgg aggcttcctt caactacctg
     4441 aagtctccaa acttcagcaa gctgatcaac atcatcatct ggttcctgct gctctccgtg
     4501 tgcctgggtt ccctgatcta ctctaccgcc gctctgggag tgctgatgtc taacctgggt
     4561 atgcctagct actgcactgg ttacagagag ggatacctga acagcaccaa cgtgaccatc
     4621 gccacctact gcaccggcag catcccatgc tccgtgtgcc tgtctggact ggacagcctg
     4681 gacacctacc cctccctgga aaccatccag atcaccatct ctagcttcaa gtgggacctg
     4741 accgccttcg gtctggtggc tgagtggttc ctggcctaca tcctgttcac caggttcttc
     4801 tacgtgctgg gcctggccgc tatcatgcag ctgttcttct cctacttcgc tgtgcacttc
     4861 atcagcaact cctggctgat gtggctgatc atcaacctgg tgcagatggc tcccatctct
     4921 gccatggtgc gcatgtacat cttcttcgcc agcttctact acgtgtggaa gtcctacgtg
     4981 cacgtggtgg acggttgcaa ctcctctacc tgcatgatgt gctacaagcg caacagggct
     5041 accagggtgg agtgcaccac catcgtgaac ggcgtgcgca ggtccttcta cgtgtacgcc
     5101 aacggcggca agggattctg caagctgcac aactggaact gcgtgaactg cgacaccttc
     5161 tgcgctggct ccaccttcat ctctgacgag gtggccagag acctgagcct gcagttcaag
     5221 cgtccaatca accccaccga ccagagctcc tacatcgtgg actctgtgac cgtgaagaac
     5281 ggtagcatcc acctgtactt cgacaaggct ggccagaaga cctacgagcg ccactctctg
     5341 agccacttcg tgaacctgga caacctgagg gccaacaaca ccaagggcag cctgcctatc
     5401 aacgtgatcg tgttcgacgg aaagtccaag tgcgaggaat ctagcgccaa gtccgccagc
     5461 gtgtactaca gccagctgat gtgccagcca atcctgctgc tggaccaggc tctggtgtct
     5521 gacgtgggag acagcgccga agtggccgtg aagatgttcg acgcctacgt gaacaccttc
     5581 tcctctacct tcaacgtgcc tatggagaag ctgaagaccc tggtggctac tgctgaggct
     5641 gaactggcta agaacgtgtc cctggacaac gtgctgagca ccttcatctc cgccgctagg
     5701 cagggtttcg tggactctga cgtggaaacc aaagacgtgg tggagtgcct gaagctgtct
     5761 caccagagcg acatcgaagt gaccggcgac tcctgcaaca actacatgct gacctacaac
     5821 aaggtggaga acatgacccc aagagacctg ggagcttgca tcgactgctc cgcccgtcac
     5881 atcaacgctc aggtggccaa gtctcacaac atcgccctga tctggaacgt gaaggacttc
     5941 atgagcctgt ccgagcagct gcgcaagcag atcaggagcg ccgctaagaa gaacaacctg
     6001 cccttcaagc tgacctgcgc taccaccaga caggtggtga acgtggtgac caccaagatc
     6061 gccctgaagg gcggactcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     6121 cgggatcccg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     6181 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     6241 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     6301 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     6361 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     6421 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     6481 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     6541 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     6601 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     6661 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     6721 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     6781 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     6841 aagtaaagcg gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac
     6901 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     6961 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     7021 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     7081 atgtatctta aggcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     7141 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     7201 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     7261 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     7321 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     7381 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     7441 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     7501 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc
     7561 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7621 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7681 agtcctgagg cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     7741 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     7801 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7861 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     7921 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     7981 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     8041 aagatcgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     8101 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     8161 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     8221 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     8281 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     8341 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     8401 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     8461 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     8521 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     8581 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     8641 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     8701 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8761 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8821 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     8881 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca
     8941 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     9001 tcctccagcg cggggatctc atgctggagt tcttcgccca ccctaggggg aggctaactg
     9061 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
     9121 ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     9181 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
     9241 ccccacccca ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg
     9301 caggccctgc catagcctca ggttactcat atatacttta gattgattta aaacttcatt
     9361 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9601 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9721 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9781 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9841 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9901 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9961 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10021 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10081 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10141 tatcccctga ttctgtggat aaccgtatta ccgccatgca ttagttatta atagtaatca
    10201 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
    10261 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
    10321 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    10381 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    10441 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    10501 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
