LOCUS       sequence-32502-e         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  TITLE     pMOTag2T
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag4HM
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        39..57
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             111..140
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             153..182
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             195..224
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             249..329
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 cacaggaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa
       61 caaaagctgg taccgggccc cccctcgagg gcggccgatc gagatccgag gagcagaagc
      121 tgatctcgga ggaggatctg ctgagatccg aggagcagaa gctgatctcg gaggaggatc
      181 tgctgagatc cgaggagcag aagctgatct cagaggagga tctgctgcga tctgaagcgg
      241 ccgtcgagta cccttacgat gtgcctgatt acgcgtaccc atacgacgtg ccagactacg
      301 catacccgta cgatgtgccc gattacgcat agtcgacaaa gtgtgacaac gtcgcaccat
      361 gtgtaggttt tcatttatgt tctttctttc tttcttttgt gaatttgttt tctgtctcaa
      421 atgtttttaa ttcgcttggg acctatgttt ttcttgtttt tttgctcacc ctttgtgtag
      481 gaggcaccct gtcacgtctg tggttgcgtg tatgccttcc ttccccttat tcgcttcttc
      541 ctgtcgtgtc acacctcttt ctccctctcc ctttccgcct tttctttcaa tcttgttttc
      601 tcgaccagcc ctactagagg agaaagaata gtaacccttt catcaaagaa aatagttcaa
      661 acgaattgct agcttaagct tgatcta
//
