LOCUS       sequence-325021-       13228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, 
            tdTomato, and human TdT..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Loveless TB, Grotts JH, Schechter MW, Forouzmand E, Carlson CK, 
            Agahi BS, Liang G, Ficht M, Liu B, Xie X, Liu CC
  TITLE     Lineage tracing and analog recording in mammalian cells by 
            single-site DNA writing.
  JOURNAL   Nat Chem Biol. 2021 Jun;17(6):739-747. doi: 
            10.1038/s41589-021-00769-8. Epub 2021 Mar 22.
  PUBMED    33753928
REFERENCE   2  (bases 1 to 13228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-021-00769-8"; journalName: "Nat Chem Biol"; date: 
            "2021-06"; volume: "17"; issue: "6"; pages: "739-747"
COMMENT     Sequence Label: pTBL1269
            pLenti-CHYRON17-pEF1a(short)-Luc2-P2A...
FEATURES             Location/Qualifiers
     source          1..13228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2320..2568
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2320..2340
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2491..2510
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    2722..2839
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2908..3119
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3139..3148
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3145..4794
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             4810..4866
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4876..6303
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGIGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             6313..6366
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    7920..8508
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7973..7993)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8391..8402)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8511..8527)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8512..8528)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9033..9213
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9239..9256)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9245..9469
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9515..9943
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9602..9621)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9812..9833
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9952..9972)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9957..10286
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10137..10272
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10199..10218
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10334..10381
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10400..10774
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10904..11025
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10941..10960)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10995..11014
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11074..11090)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11074..11090)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11087..11109)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11098..11114
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11122..11128,11129..11146,11147..11152))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11167..11188
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11305..11322)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11476..12064)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11556..11575)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12235..13026,13027..13095))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12858..12877
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13096..13200)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccagag agggcctatt tcccatgatt
     2341 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
     2401 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     2461 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     2521 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc agactgagca
     2581 cgtgagggtt agagctagaa atagcaagtt aacctaaggc tagtccgtta tcaacttgaa
     2641 aaagtggcac cgagtcggtg ctttttttgt ttatccatat gaattctaga tcttgagaca
     2701 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     2761 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     2821 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg
     2881 gctcgagtgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     2941 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     3001 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     3061 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     3121 tgtcgtgacg cgggatccgc caccatggaa gatgccaaaa acattaagaa gggcccagcg
     3181 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc
     3241 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc
     3301 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg
     3361 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg
     3421 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc
     3481 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg
     3541 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg
     3601 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg
     3661 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc
     3721 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac
     3781 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc
     3841 cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg
     3901 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta
     3961 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt
     4021 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc
     4081 gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac
     4141 ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc
     4201 cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag
     4261 gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc
     4321 cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc
     4381 gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc
     4441 ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc
     4501 gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg
     4561 cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc
     4621 atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg
     4681 cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc
     4741 cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgaagctt
     4801 ggaagcggag ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct
     4861 ggacctcccg ggccaatggt gagcaagggc gaggaggtca tcaaagagtt catgcgcttc
     4921 aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     4981 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggccccctg
     5041 cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc gtacgtgaag
     5101 caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt caagtgggag
     5161 cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc ctccctgcag
     5221 gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc cgacggcccc
     5281 gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta cccccgcgac
     5341 ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg ccactacctg
     5401 gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg ctactactac
     5461 gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5521 gagcgctccg agggccgcca ccacctgttc ctggggcatg gcatcggcag caccggcagc
     5581 ggcagctccg gcaccgcctc ctccgaggac aacaacatgg ccgtcatcaa agagttcatg
     5641 cgcttcaagg tgcgcatgga gggctccatg aacggccacg agttcgagat cgagggcgag
     5701 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc
     5761 cccctgccct tcgcctggga catcctgtcc ccccagttca tgtacggctc caaggcgtac
     5821 gtgaagcacc ccgccgacat ccccgattac aagaagctgt ccttccccga gggcttcaag
     5881 tgggagcgcg tgatgaactt cgaggacggc ggtctggtga ccgtgaccca ggactcctcc
     5941 ctgcaggacg gcacgctgat ctacaaggtg aagatgcgcg gcaccaactt cccccccgac
     6001 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg cctgtacccc
     6061 cgcgacggcg tgctgaaggg cgagatccac caggccctga agctgaagga cggcggccac
     6121 tacctggtgg agttcaagac catctacatg gccaagaagc ccgtgcaact gcccggctac
     6181 tactacgtgg acaccaagct ggacatcacc tcccacaacg aggactacac catcgtggaa
     6241 cagtacgagc gctccgaggg ccgccaccac ctgttcctgt acggcatgga cgagctgtac
     6301 aagggcagcg gcgagggcag aggcagcctg ctgacctgcg gcgacgtgga ggagaacccc
     6361 ggccccatgg atccaccacg agcgtcccac ttgagccctc ggaagaagag accccggcag
     6421 acgggtgcct tgatggcctc ctctcctcaa gacatcaaat ttcaagattt ggtcgtcttc
     6481 attttggaga agaaaatggg aaccacccgc agagcgttcc tcatggagct ggcccgcagg
     6541 aaagggttca gggttgaaaa tgagctcagt gattctgtca cccacattgt agcagagaac
     6601 aactcgggtt cggatgttct ggagtggctt caagcacaga aagtacaagt cagctcacaa
     6661 ccagagctcc tcgatgtctc ctggctgatc gaatgcatag gagcagggaa accggtggaa
     6721 atgacaggaa aacaccagct tgttgtgaga agagactatt cagatagcac caacccaggc
     6781 cccccgaaga ctccaccaat tgctgtacaa aagatctccc agtatgcgtg tcagagaaga
     6841 accactttaa acaactgtaa ccagatattc acggatgcct ttgatatact ggctgaaaac
     6901 tgtgagttta gagaaaatga agactcctgt gtgacattta tgagagcagc ttctgtattg
     6961 aaatctctgc cattcacaat catcagtatg aaggacacag aagggattcc ctgcctgggg
     7021 tccaaggtga agggtatcat agaggagatt attgaagatg gagaaagttc tgaagttaaa
     7081 gctgtgttaa atgatgaacg atatcaatcc ttcaaactct ttacttctgt atttggagtg
     7141 gggctgaaga cttctgagaa gtggttcagg atgggtttca gaactctgag taaagtaagg
     7201 tcggacaaaa gcctgaaatt tacacgaatg cagaaagcag gatttctgta ttatgaagac
     7261 cttgtaagct gtgtgaccag ggcagaagca gaggccgtca gtgtgctggt taaagaggct
     7321 gtctgggcat ttcttccgga tgctttcgtc accatgacag gagggttccg gaggggtaag
     7381 aagatggggc atgatgtaga ttttttaatt accagcccag gatcaacaga ggatgaagag
     7441 caacttttac agaaagtgat gaacttatgg gaaaagaagg gattactttt atattatgac
     7501 cttgtggagt caacatttga aaagctcagg ttgcctagca ggaaggttga tgctttggat
     7561 cattttcaaa agtgctttct gattttcaaa ttgcctcgtc aaagagtgga cagtgaccag
     7621 tccagctggc aggaaggaaa gacctggaag gccatccgtg tggatttagt tctgtgcccc
     7681 tacgagcgtc gtgcctttgc cctgttggga tggactggct cccggcagtt tgagagagac
     7741 ctccggcgct atgccacaca tgagcggaag atgattctgg ataaccatgc tttatatgac
     7801 aagaccaaga ggatattcct caaagcagaa agtgaagaag aaatttttgc gcatctggga
     7861 ttggattata ttgaaccgtg ggaaagaaat gcctaaaatt cgatatcaag cttatcggta
     7921 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     7981 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     8041 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     8101 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     8161 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     8221 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     8281 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     8341 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     8401 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     8461 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     8521 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     8581 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     8641 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     8701 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     8761 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     8821 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     8881 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     8941 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     9001 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     9061 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9121 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9181 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     9241 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     9301 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     9361 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     9421 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     9481 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     9541 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9601 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9661 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9721 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9781 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9841 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     9901 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     9961 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    10021 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
    10081 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
    10141 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
    10201 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
    10261 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
    10321 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
    10381 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
    10441 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
    10501 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
    10561 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
    10621 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
    10681 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
    10741 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
    10801 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
    10861 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
    10921 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10981 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
    11041 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    11101 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    11161 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    11221 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
    11281 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
    11341 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
    11401 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
    11461 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
    11521 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
    11581 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
    11641 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
    11701 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
    11761 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
    11821 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
    11881 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
    11941 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
    12001 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
    12061 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    12121 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    12181 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    12241 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    12301 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    12361 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    12421 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    12481 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    12541 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    12601 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    12661 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    12721 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    12781 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    12841 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    12901 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    12961 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13021 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13081 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    13141 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    13201 cacatttccc cgaaaagtgc cacctgac
//
