LOCUS       sequence-325026-       10364 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10364)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtPSY2:T35s
FEATURES             Location/Qualifiers
     source          1..10364
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        239..343
                     /gene="bla"
                     /label=AmpR promoter
     CDS             344..1138
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1309..1897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1798..1817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    2020..2044
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      4297..4549
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    6012..6186
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6286..6465
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6487..7281
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6541..6560)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7151..7170
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7294..7546
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7641..7665
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7929..8558
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8987..10060
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10126..10320
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc gcggccgctg
       61 gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga caagccgcgc
      121 cgtcgccact cgaccgccgg cgcccacatc aaggcaccga gctcccatcc ccccaggggc
      181 tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agcgctggcc aattcgtgcg
      241 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      301 ataaccttga taaatgcttc aataatattg aaaaaggaag agtatggcta aaatgagaat
      361 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gcaaaagata cggaaggaat
      421 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
      481 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
      541 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
      601 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
      661 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
      721 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
      781 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaggaca ctccatttaa
      841 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
      901 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
      961 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     1021 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     1081 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagct
     1141 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     1201 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     1261 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1321 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1381 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1441 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1501 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1561 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1621 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1681 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1741 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1801 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1861 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1921 acggttcctg tggttggcac atacaaatgg acgaacggat aaaccttttc acgccctttt
     1981 aaatatccga ttattctaat aaacgctctt ttctcttagg tttacccgcc aatatatcct
     2041 gtcaaacact gatagtttaa actccctcgg tcacatgtgc atcctcgtaa ggaggacgtc
     2101 gttgtggttg gtgctttcct tacattctga gcctctttcc ttctaatcca ctcatctgca
     2161 tcttcttgtg tccttactaa tacctcattg gttccaaatt ccctcccttt aagcaccagc
     2221 tcgtttctgt tcttccacag cctcccaagt atccaaggga ctaaagcctc cacattcttc
     2281 agatcaggat attcttgttt aagatgttga actctatgga ggtttgtatg aactgatgat
     2341 ctaggaccgg ataagttccc ttcttcatag cgaacttatt caaagaatgt tttgtgtatc
     2401 attcttgtta cattgttatt aatgaaaaaa tattattggt cattggactg aacacgagtg
     2461 ttaaatatgg accaggcccc aaataagatc cattgatata tgaattaaat aacaagaata
     2521 aatcgagtca ccaaaccact tgcctttttt aacgagactt gttcaccaac ttgatacaaa
     2581 agtcattatc ctatgcaaat caataatcat acaaaaatat ccaataacac taaaaaaatt
     2641 aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat
     2701 tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa
     2761 gaaataaagc acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac
     2821 ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct
     2881 aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt
     2941 aaggaaattg tggttgtcgg acgaagtcca gtaataaacg gcgtcaaagt ggttgcagcc
     3001 ggcacacacg agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat
     3061 aaggcaatta gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta
     3121 ttattagcta ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct
     3181 tcttcttctt ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa
     3241 tttctcaaaa tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct
     3301 gtgttcctta ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat
     3361 gttctttggt ttagattctg ttaatcttag atcgtagacg attttctggg tttgatcgtt
     3421 agatatcatc ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat
     3481 ttgagttttg tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt
     3541 ttctagtttg tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagatgcag
     3601 atcttaatgg ggtcatccaa gaatgttatc aaggagttca tgaggtttaa ggttcgcatg
     3661 gaaggaacgg tcaatgggca cgagtttgaa atagaaggcg aaggagaggg gaggccatac
     3721 gaaggccaca ataccgtaaa gcttaaggta accaaggggg gacctttgcc atttgcttgg
     3781 gatattttgt caccacaatt tcagtatgga agcaaggtat atgtcaagca ccctgccgac
     3841 ataccagact ataaaaagct gtcatttcct gaaggattta aatgggaaag ggtcatgaac
     3901 tttgaagatg gtggcgtcgt tactgtaacc caggattcca gtttgcagga tggctgtttc
     3961 atctacaagg tcaagttcat tggcgtgaac tttccttccg atggacctgt tatgcaaaag
     4021 aaaacaatgg gctgggaagc cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa
     4081 ggagagattc ataaggctct gaagctgaaa gacggtggtc attacctagt tgaattcaaa
     4141 agtatttaca tggcaaagaa gcctgtgcag ctaccagggt actactatgt tgactccaaa
     4201 ctggatataa caagccacaa cgaagattat acaatcgttg agcagtatga aagaaccgag
     4261 ggacgccacc atctgttcct ttaagcttgt caagcagatc gttcaaacat ttggcaataa
     4321 agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg
     4381 aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt
     4441 tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc
     4501 gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg acgctgagct
     4561 cgaattctag tggagggaga gaggattttg aggaaataat taattgaatt acttgtatta
     4621 ttgataaagt aatttagata agttgttagt acaacttatt gcaactatgg atagacaaaa
     4681 atcacttatt ttaaggggag aagtatataa caacttatta taaaatttct gagcagccgg
     4741 cctcctccaa tcaatcatag agagtggagc ccattctgga aaaccaagga acccccaact
     4801 tgcactggtg cggtggccca attcaaaaaa aacggggccc agcaaagtaa cgccgttgta
     4861 acgatttatc aatccaaact caaaaggcgg cgagatgcgt ttaactccgt gaaattaaca
     4921 aaccgccaac aacttgcaat ttgcaactac cgtttccaag aagaactcaa ccacacaacg
     4981 tatcctatcc caaaccacac gcaactagtg acgcgtcata aggacacgtg tcacaatttg
     5041 actggttaat aatttcaccg cttttgctat aaattacctc caatcccctt agcttcttca
     5101 caattcagtt cccaacccta acaattcttg ttcatatcgc ttctctctac tttcaaggta
     5161 tgatccaatt tctctcttct ttctctgtaa tcctttcgtt gagttttgtt tccgatcaat
     5221 cataggtagt tttcttgttt cgaagcatga gatctaggaa ttttttgtga ttttccaaaa
     5281 ttgagatcgg tttgaaattg aattttacag cttgaatctc agatcttgtt ttatcaatgt
     5341 tttcgatggc ttgcgatgta gatctatgat aattgtggtt cagttttgtt aggaatcgat
     5401 ttcggtttag caattgcaga ttaattaggg tttccaattg aattcttcag atccgttatg
     5461 gaattatgtc aaataattta ttcaaattgg aaattattgt tagatccact cttaatctgt
     5521 ttgatccaag cttcaattag ggttttcact tgtttcaatt tcttgttatg gattctgatt
     5581 tatctgttga tgttagatcc actcttaatc tgtttgatcc aagcgttaat tagggttttc
     5641 acttgtttca atttcgtgtg ttggattctg atttatctgt tgttgatgtg attacagaat
     5701 gaaaggcaat atttttcacc tgtttgtcat ttttttaggg ctttcccatc ttgtatgctt
     5761 caaagcagtt ccaattacaa gaagtgaaag ccttatacaa cgtgctcaaa ttcatcttgt
     5821 attggagaat gccaacaatc acaaggatat ggtcatcaat gaaagaaact ggccgcgaga
     5881 ggagcctacc gttgttgaga ggatgaatct ggaactccat gactacccgc catcaggggc
     5941 taatggtcgt cacactccaa gagtaccata tccatgagct tctctagcta gagtcgatcg
     6001 acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg aattagggtt
     6061 cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     6121 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     6181 cagatcgcta agcttgaatt cttgcggaga gcggagaatt aagggagtca cgttatgacc
     6241 cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc gcaacgttga
     6301 aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac gtcagaaacc
     6361 attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat ttcttgtcaa
     6421 aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc tcatattcac
     6481 tctcaaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     6541 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     6601 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     6661 gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     6721 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     6781 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     6841 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     6901 catcgcatcg agcgaggacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     6961 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcggatg
     7021 cccgacggcg aggatctcgt cgtgacccag ggcgatgcct gcttgccgaa tatcatggtg
     7081 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     7141 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     7201 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     7261 cttcttgacg agttcttctg agcttgtcaa gcagatcgtt caaacatttg gcaataaagt
     7321 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     7381 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     7441 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     7501 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg ctggatccga
     7561 attcggcagg gcccactgca tccaccccag tacattaaaa acgtccgcaa tgtgttatta
     7621 agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag
     7681 ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagccgtg
     7741 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     7801 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     7861 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     7921 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     7981 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     8041 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     8101 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     8161 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     8221 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     8281 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     8341 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     8401 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     8461 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     8521 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     8581 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     8641 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     8701 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     8761 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     8821 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     8881 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct
     8941 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     9001 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     9061 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     9121 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc
     9181 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     9241 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     9301 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     9361 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     9421 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     9481 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     9541 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     9601 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     9661 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     9721 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     9781 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     9841 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     9901 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     9961 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    10021 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    10081 gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta
    10141 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    10201 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    10261 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    10321 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg ccta
//
