LOCUS       sequence-325028-       10430 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10430)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10430)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtnsLTP17:T35s
FEATURES             Location/Qualifiers
     source          1..10430
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             100..1173
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1239..1433
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1716..1820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1821..2615
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2786..3374
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3275..3294
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3497..3521
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      5774..6026
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7555..7729
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        7829..8008
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8030..8824
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8084..8103)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8694..8713
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8837..9089
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9184..9208
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9472..10101
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
       61 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc
      121 ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg
      181 caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg
      241 gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc gtcgtcgatt
      301 aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct ctatgacgtg
      361 ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc gaagcgtgac
      421 cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga ggtttccgca
      481 gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc ggtttcccat
      541 ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg ccgcgtgttc
      601 cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg aaagcagaaa
      661 gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat gcagcgtacg
      721 aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt gattagccgc
      781 tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga gctagctgat
      841 tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt tcaccccgat
      901 tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg ccgcgccgca
      961 ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg cagcgccgga
     1021 gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga cctgccggag
     1081 tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac
     1141 ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct agggcaaatt
     1201 gccctagcag gggaaaaagg tcgaaaaagc ttctttcctg tggatagcac gtacattggg
     1261 aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa gccgtacatt
     1321 gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga tttttccgcc
     1381 taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc ataactgtct
     1441 ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta
     1501 cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat ggctggccta
     1561 cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc
     1621 ccacatcaag gcaccgagct cccatccccc caggggctgc gcccctcggc cgcgaacggc
     1681 ctcaccccaa aaatggcagc gctggccaat tcgtgcgcgg aacccctatt tgtttatttt
     1741 tctaaataca ttcaaatatg tatccgctca tgagacaata accttgataa atgcttcaat
     1801 aatattgaaa aaggaagagt atggctaaaa tgagaatatc accggaattg aaaaaactga
     1861 tcgaaaaata ccgctgcgca aaagatacgg aaggaatgtc tcctgctaag gtatataagc
     1921 tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat aaagggacca
     1981 cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag ctgcctgttc
     2041 caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg agtgaggccg
     2101 atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag attatcgagc
     2161 tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt ccctatacga
     2221 atagcttaga cagccgctta gccgaattgg attacttact gaataacgat ctggccgatg
     2281 tggattgcga aaactgggaa gaggacactc catttaaaga tccgcgcgag ctgtatgatt
     2341 ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac ctgggagaca
     2401 gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg agaagcggca
     2461 gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag gatatcgggg
     2521 aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa
     2581 taaaatatta tattttactg gatgaattgt tttagctgtc agaccaagtt tactcatata
     2641 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2701 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     2761 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     2821 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     2881 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     2941 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     3001 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     3061 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     3121 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     3181 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     3241 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     3301 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     3361 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctgtgg ttggcacata
     3421 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa
     3481 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
     3541 ccctcggtca catgtgcatc ctcgtaagga ggacgtcgtt gtggttggtg ctttccttac
     3601 attctgagcc tctttccttc taatccactc atctgcatct tcttgtgtcc ttactaatac
     3661 ctcattggtt ccaaattccc tccctttaag caccagctcg tttctgttct tccacagcct
     3721 cccaagtatc caagggacta aagcctccac attcttcaga tcaggatatt cttgtttaag
     3781 atgttgaact ctatggaggt ttgtatgaac tgatgatcta ggaccggata agttcccttc
     3841 ttcatagcga acttattcaa agaatgtttt gtgtatcatt cttgttacat tgttattaat
     3901 gaaaaaatat tattggtcat tggactgaac acgagtgtta aatatggacc aggccccaaa
     3961 taagatccat tgatatatga attaaataac aagaataaat cgagtcacca aaccacttgc
     4021 cttttttaac gagacttgtt caccaacttg atacaaaagt cattatccta tgcaaatcaa
     4081 taatcataca aaaatatcca ataacactaa aaaaattaaa agaaatggat aatttcacaa
     4141 tatgttatac gataaagaag ttacttttcc aagaaattca ctgattttat aagcccactt
     4201 gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa ataaagcacg aagaattcta
     4261 gaaaatacga aatacgcttc aatgcagtgg gacccacggt tcaattattg ccaattttca
     4321 gctccaccgt atatttaaaa aataaaacga taatgctaaa aaaatataaa tcgtaacgat
     4381 cgttaaatct caacggctgg atcttatgac gaccgttaag gaaattgtgg ttgtcggacg
     4441 aagtccagta ataaacggcg tcaaagtggt tgcagccggc acacacgagt cgtgtttatc
     4501 aactcaaagc acaaatactt ttcctcaacc taaaaataag gcaattagcc aaaaacaact
     4561 ttgcgtgtaa acaacgctca atacacgtgt cattttatta ttagctattg cttcaccgcc
     4621 ttagctttct cgtgacctag tcgtcctcgt cttttcttct tcttcttcta taaaacaata
     4681 cccaaagagc tcttcttctt cacaattcag atttcaattt ctcaaaatct taaaaacttt
     4741 ctctcaattc tctctaccgt gatcaaggta aatttctgtg ttccttattc tctcaaaatc
     4801 ttcgattttg ttttcgttcg atcccaattt cgtatatgtt ctttggttta gattctgtta
     4861 atcttagatc gtagacgatt ttctgggttt gatcgttaga tatcatctta attctcgatt
     4921 agggtttcat agatatcatc cgatttgttc aaataatttg agttttgtcg aataattact
     4981 cttcgatttg tgatttctat ctagatctgg tgttagtttc tagtttgtgc gatcgaattt
     5041 gtcgattaat ctgagttttt ctgattaaca gatgcagatc ttaatggggt catccaagaa
     5101 tgttatcaag gagttcatga ggtttaaggt tcgcatggaa ggaacggtca atgggcacga
     5161 gtttgaaata gaaggcgaag gagaggggag gccatacgaa ggccacaata ccgtaaagct
     5221 taaggtaacc aaggggggac ctttgccatt tgcttgggat attttgtcac cacaatttca
     5281 gtatggaagc aaggtatatg tcaagcaccc tgccgacata ccagactata aaaagctgtc
     5341 atttcctgaa ggatttaaat gggaaagggt catgaacttt gaagatggtg gcgtcgttac
     5401 tgtaacccag gattccagtt tgcaggatgg ctgtttcatc tacaaggtca agttcattgg
     5461 cgtgaacttt ccttccgatg gacctgttat gcaaaagaaa acaatgggct gggaagccag
     5521 cactgagcgt ttgtatcctc gtgatggcgt gttgaaagga gagattcata aggctctgaa
     5581 gctgaaagac ggtggtcatt acctagttga attcaaaagt atttacatgg caaagaagcc
     5641 tgtgcagcta ccagggtact actatgttga ctccaaactg gatataacaa gccacaacga
     5701 agattataca atcgttgagc agtatgaaag aaccgaggga cgccaccatc tgttccttta
     5761 agcttgtcaa gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt
     5821 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat
     5881 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt
     5941 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg
     6001 cgcggtgtca tctatgttac tagatcgacg ctgagctcga attctagtgg agggagagag
     6061 gattttgagg aaataattaa ttgaattact tgtattattg ataaagtaat ttagataagt
     6121 tgttagtaca acttattgca actatggata gacaaaaatc acttatttta aggggagaag
     6181 tatataacaa cttattataa aatttctgag cagccggcct cctccaatca atcatagaga
     6241 gtggagccca ttctggaaaa ccaaggaacc cccaacttgc actggtgcgg tggcccaatt
     6301 caaaaaaaac ggggcccagc aaagtaacgc cgttgtaacg atttatcaat ccaaactcaa
     6361 aaggcggcga gatgcgttta actccgtgaa attaacaaac cgccaacaac ttgcaatttg
     6421 caactaccgt ttccaagaag aactcaacca cacaacgtat cctatcccaa accacacgca
     6481 actagtgacg cgtcataagg acacgtgtca caatttgact ggttaataat ttcaccgctt
     6541 ttgctataaa ttacctccaa tccccttagc ttcttcacaa ttcagttccc aaccctaaca
     6601 attcttgttc atatcgcttc tctctacttt caaggtatga tccaatttct ctcttctttc
     6661 tctgtaatcc tttcgttgag ttttgtttcc gatcaatcat aggtagtttt cttgtttcga
     6721 agcatgagat ctaggaattt tttgtgattt tccaaaattg agatcggttt gaaattgaat
     6781 tttacagctt gaatctcaga tcttgtttta tcaatgtttt cgatggcttg cgatgtagat
     6841 ctatgataat tgtggttcag ttttgttagg aatcgatttc ggtttagcaa ttgcagatta
     6901 attagggttt ccaattgaat tcttcagatc cgttatggaa ttatgtcaaa taatttattc
     6961 aaattggaaa ttattgttag atccactctt aatctgtttg atccaagctt caattagggt
     7021 tttcacttgt ttcaatttct tgttatggat tctgatttat ctgttgatgt tagatccact
     7081 cttaatctgt ttgatccaag cgttaattag ggttttcact tgtttcaatt tcgtgtgttg
     7141 gattctgatt tatctgttgt tgatgtgatt acagaatggg taaggttgca ttatttgttt
     7201 tgatggcttg catgatgata gcaagttctc atgctaaagc aaccctaaca tgtgagcaaa
     7261 caaccatata cctaactcca tgcataccct atggaatgtt gggaggaagt gtatcaccct
     7321 tgtgttgcca aggtattcac tctataaatg ctgcatataa gaatggcgat gatagaagag
     7381 ttgcatgcca ttgcattcag gatacagctg ctaaaatccc tttgattgat tatacaagaa
     7441 ttaatcaaat tggtgaacta tgtggatcta aatgtccctt caaagtttac ccctccactg
     7501 attgcaataa ggtgaagtaa gcttctctag ctagagtcga tcgacaagct cgagtttctc
     7561 cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc
     7621 atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca
     7681 ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcg ctaagcttga
     7741 attcttgcgg agagcggaga attaagggag tcacgttatg acccccgccg atgacgcggg
     7801 acaagccgtt ttacgtttgg aactgacaga accgcaacgt tgaaggagcc actcagccgc
     7861 gggtttctgg agtttaatga gctaagcaca tacgtcagaa accattattg cgcgttcaaa
     7921 agtcgcctaa ggtcactatc agctagcaaa tatttcttgt caaaaatgct ccactgacgt
     7981 tccataaatt cccctcggta tccaattaga gtctcatatt cactctcaaa tgattgaaca
     8041 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     8101 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     8161 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aagacgaggc
     8221 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     8281 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     8341 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     8401 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagg
     8461 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     8521 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgg atgcccgacg gcgaggatct
     8581 cgtcgtgacc cagggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     8641 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     8701 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     8761 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     8821 ctgagcttgt caagcagatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc
     8881 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat
     8941 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca
     9001 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc
     9061 gcgcgcggtg tcatctatgt tactagatcg acgctggatc cgaattcggc agggcccact
     9121 gcatccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     9181 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     9241 cggcacaaaa tcaccactcg atacaggcag cccatcagcc gtgcggctgc atgaaatcct
     9301 ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac
     9361 cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt
     9421 cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt
     9481 atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc
     9541 cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt
     9601 gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc
     9661 ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga
     9721 gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt
     9781 ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag
     9841 cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc gcgggcgatc
     9901 aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt
     9961 cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc
    10021 gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa
    10081 attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca
    10141 cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg
    10201 cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc
    10261 tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg
    10321 cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa
    10381 ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
//
