LOCUS       sequence-32503-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  TITLE     pMOTag2T
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMOTag5H
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        30..48
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             81..161
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             518..892
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 agctatgacc atgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg
       61 gtaccgggcc ccccctcgag tacccttacg atgtgcctga ttacgcgtac ccatacgacg
      121 tgccagacta cgcatacccg tacgatgtgc ccgattacgc atagtcgaca aagtgtgaca
      181 acgtcgcacc atgtgtaggt tttcatttat gttctttctt tctttctttt gtgaatttgt
      241 tttctgtctc aaatgttttt aattcgcttg ggacctatgt ttttcttgtt tttttgctca
      301 ccctttgtgt aggaggcacc ctgtcacgtc tgtggttgcg tgtatgcctt ccttcccctt
      361 attcgcttct tcctgtcgtg tcacacctct ttctccctct ccctttccgc cttttctttc
      421 aatcttgttt tctcgaccag ccctactaga ggagaaagaa tagtaaccct ttcatcaaag
      481 aaaatagttc aaacgaattg ctagcttaag cttgatcatg gccaagttga ccagtgccgt
      541 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
      601 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
      661 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
      721 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
      781 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
      841 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact ga
//
