LOCUS       sequence-325030-       11517 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11517)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 11517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtFam27_1:T35s
FEATURES             Location/Qualifiers
     source          1..11517
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     345..362
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    479..500
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(515..520,521..538,539..545)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    553..569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     558..580
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     577..593
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     577..593
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2869..3043
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        3143..3322
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             3344..4138
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3398..3417)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4008..4027
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4151..4403
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4498..4522
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4786..5415
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5844..6917
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      6983..7177
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        7460..7564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7565..8359
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8530..9118
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    9241..9265
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     complement(9342..9359)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9342..9358)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9351..9373)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9567..9586)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9686..9708
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9746..9764)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9832..9936
                     /gene="bla"
                     /label=AmpR promoter
     CDS             10158..10949
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(11120..11517,1..191)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
       61 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      241 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      301 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      361 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      421 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      481 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      541 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
      601 acgccaagct tgcatgcctg caggtcgact ctagaggatc ccctttgggc ccaggaagtt
      661 cggcgttgct gggtattgcc cccatgcccc cgcttatttc atttggttcg gcatcacctt
      721 cagctctcat tgactcctgg atttcagcta ggcgtttagc tttctttgcg gcctggtgtt
      781 tcacctccct actgtgtttc ctgtggagct tgttgaggaa agtttctgca gggtttgagt
      841 attgatttct gtctcctgca ggttctttaa cttcttgacc gacatccatc tcgggcgttt
      901 tcctgattgc agctaaatct tcctctgcgt ttaccgcata agctggcatt cttgtgaagc
      961 aatcaagcaa gtccccatct gagaacaaac tttccatttc ctctacatcc gacacttccc
     1021 ttggtcggaa ggctttccac gcttgcaagg ggtggaatct gaagtcccaa gtttccacga
     1081 agacaatccc tcggtcacat gtgcatcctc tagtggaggg agagaggatt ttgaggaaat
     1141 aattaattga attacttgta ttattgataa agtaatttag ataagttgtt agtacaactt
     1201 attgcaacta tggatagaca aaaatcactt attttaaggg gagaagtata taacaactta
     1261 ttataaaatt tctgagcagc cggcctcctc caatcaatca tagagagtgg agcccattct
     1321 ggaaaaccaa ggaaccccca acttgcactg gtgcggtggc ccaattcaaa aaaaacgggg
     1381 cccagcaaag taacgccgtt gtaacgattt atcaatccaa actcaaaagg cggcgagatg
     1441 cgtttaactc cgtgaaatta acaaaccgcc aacaacttgc aatttgcaac taccgtttcc
     1501 aagaagaact caaccacaca acgtatccta tcccaaacca cacgcaacta gtgacgcgtc
     1561 ataaggacac gtgtcacaat ttgactggtt aataatttca ccgcttttgc tataaattac
     1621 ctccaatccc cttagcttct tcacaattca gttcccaacc ctaacaattc ttgttcatat
     1681 cgcttctctc tactttcaag gtatgatcca atttctctct tctttctctg taatcctttc
     1741 gttgagtttt gtttccgatc aatcataggt agttttcttg tttcgaagca tgagatctag
     1801 gaattttttg tgattttcca aaattgagat cggtttgaaa ttgaatttta cagcttgaat
     1861 ctcagatctt gttttatcaa tgttttcgat ggcttgcgat gtagatctat gataattgtg
     1921 gttcagtttt gttaggaatc gatttcggtt tagcaattgc agattaatta gggtttccaa
     1981 ttgaattctt cagatccgtt atggaattat gtcaaataat ttattcaaat tggaaattat
     2041 tgttagatcc actcttaatc tgtttgatcc aagcttcaat tagggttttc acttgtttca
     2101 atttcttgtt atggattctg atttatctgt tgatgttaga tccactctta atctgtttga
     2161 tccaagcgtt aattagggtt ttcacttgtt tcaatttcgt gtgttggatt ctgatttatc
     2221 tgttgttgat gtgattacag aatggaggct ttttcttcaa agatgcttct aataattttt
     2281 gcctttagtt tcattacaca ctttatccaa gcaattgaaa gaccatacca acaagcaaac
     2341 acattattca ttaaaaattc ttgcagctct acaacttatc caagactatg ctacacctcc
     2401 ctagtgaagc acgctgattc cattcaaacg aatcacgtgc ttttaacatg tacggcccta
     2461 aacgtgactc ttgcgtccgc gaaatcaaca tcggctatga tatcaactct ctcaaaaagc
     2521 caggggctga agccaagaga ggctgcagct atgaaggatt gcgtcgaaga gctgagtgac
     2581 tccgtggatg aactacgaag gtctatcggt gaaatgagtc gccttaggac ttcaaatttc
     2641 gagttaacaa tgagtgatgt acaaacttgg gtcagtgccg ctttgacgga tgagagtact
     2701 tgtaccgacg gattccaaga ggttaatgct ccgggtaatg ttcagacaac agtgagaggg
     2761 aaaattgtgc aagtagctca attgactagc aatgctttgg ctttgataaa taaacttgcc
     2821 acttcacatg aatggcttct ctagctagag tcgatcgaca agctcgagtt tctccataat
     2881 aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt
     2941 tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa
     3001 tttctaattc ctaaaaccaa aatccagtac taaaatccag atcgctaagc ttgaattctt
     3061 gcggagagcg gagaattaag ggagtcacgt tatgaccccc gccgatgacg cgggacaagc
     3121 cgttttacgt ttggaactga cagaaccgca acgttgaagg agccactcag ccgcgggttt
     3181 ctggagttta atgagctaag cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc
     3241 ctaaggtcac tatcagctag caaatatttc ttgtcaaaaa tgctccactg acgttccata
     3301 aattcccctc ggtatccaat tagagtctca tattcactct caaatgattg aacaagatgg
     3361 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     3421 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     3481 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctccaagacg aggcagcgcg
     3541 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     3601 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     3661 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     3721 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gaggacgtac
     3781 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     3841 gccagccgaa ctgttcgcca ggctcaaggc gcggatgccc gacggcgagg atctcgtcgt
     3901 gacccagggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     3961 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     4021 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     4081 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     4141 ttgtcaagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     4201 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     4261 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     4321 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     4381 ggtgtcatct atgttactag atcgacgctg gatccgaatt cggcagggcc cactgcatcc
     4441 accccagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt
     4501 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac
     4561 aaaatcacca ctcgatacag gcagcccatc agccgtgcgg ctgcatgaaa tcctggccgg
     4621 tttgtctgat gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg
     4681 ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc
     4741 gtatatgatg cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct
     4801 gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc
     4861 ctgcaactcg ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc
     4921 gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg
     4981 attgaccgcg acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc
     5041 caggcggcgg acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg
     5101 cagccaagcc cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc
     5161 attgaggtca cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc
     5221 acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag
     5281 tcccgtatca cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt
     5341 gaatcagaac ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa
     5401 tcaaaactca tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa
     5461 gtgccggccg tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca
     5521 cgccagccat gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga
     5581 tgtacgcggt acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc
     5641 taccagagta aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc
     5701 ggcatggaaa atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga
     5761 acgggcggtt ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga
     5821 acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg
     5881 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
     5941 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
     6001 gcaaagaatc ccggcaaccg ccggcagccg gtgcgtcgtc gattaggaag ccgcccaagg
     6061 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
     6121 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
     6181 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
     6241 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
     6301 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
     6361 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
     6421 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
     6481 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
     6541 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
     6601 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
     6661 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
     6721 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
     6781 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
     6841 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
     6901 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
     6961 aaggtcgaaa aagcttcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
     7021 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
     7081 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
     7141 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
     7201 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
     7261 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
     7321 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccg
     7381 agctcccatc cccccagggg ctgcgcccct cggccgcgaa cggcctcacc ccaaaaatgg
     7441 cagcgctggc caattcgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7501 tatgtatccg ctcatgagac aataaccttg ataaatgctt caataatatt gaaaaaggaa
     7561 gagtatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg
     7621 cgcaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga
     7681 aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg
     7741 ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt
     7801 tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc
     7861 ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat
     7921 caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg
     7981 cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg
     8041 ggaagaggac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa
     8101 gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa
     8161 agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta
     8221 tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga
     8281 gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt
     8341 actggatgaa ttgttttagc tgtcagacca agtttactca tatatacttt agattgattt
     8401 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     8461 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     8521 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     8581 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     8641 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     8701 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8761 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8821 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8881 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8941 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     9001 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     9061 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     9121 cgccagcaac gcggcctttt tacggttcct gtggttggca catacaaatg gacgaacgga
     9181 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
     9241 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactccctcg gtcacatgtg
     9301 catcctctag tttgtcttca aagggtaccg agctcgaatt cactggccgt cgttttacaa
     9361 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     9421 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     9481 agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     9541 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9601 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9661 gcttacagac aagctgtgac catctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9721 tcaccgaaac gcgcgagtcg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9781 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     9841 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9901 tgataaatgc ttcaataata ttgaaaaagg aagagtatgc gctcacgcaa ctggtccaga
     9961 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
    10021 tgtttttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg
    10081 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta
    10141 aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact caactatcag
    10201 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg
    10261 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga
    10321 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg
    10381 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg
    10441 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca
    10501 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc
    10561 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg
    10621 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact
    10681 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt
    10741 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc
    10801 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag
    10861 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag
    10921 gcgagatcac caaggtagtc ggcaaataac tgtcagacca agtttactca tatatacttt
    10981 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    11041 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    11101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    11161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    11221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    11281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    11341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    11401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    11461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgag
//
