LOCUS       sequence-325033-        8693 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used as a measure of cPLA2 activation in live cells with an EGFP 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8693)
  AUTHORS   Enyedi B, Jelcic M, Niethammer P
  TITLE     The Cell Nucleus Serves as a Mechanotransducer of Tissue 
            Damage-Induced Inflammation.
  JOURNAL   Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016.
  PUBMED    27203112
REFERENCE   2  (bases 1 to 8693)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-19- 
            19"; volume: "165"; issue: "5"; pages: "1160-1170"
COMMENT     Sequence Label: pSB-CMV-MCS-Puro cPLA2-EGFP
FEATURES             Location/Qualifiers
     source          1..8693
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        31..410
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        411..614
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     565..585
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        659..677
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(2994..2996,2997..2999,3000..3713)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2994..2996,2997..2999,3000..3710)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3039..3060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3300..3319)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3647..3668
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3753..3770)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3759..3983
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        3990..4347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3990..4181
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4013..4033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4198..4333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4375..4974
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4375..4394)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4871..4891
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(4988..5007)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5017..5098
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5042..5061
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5573..5593)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5573..5591)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5612..5628)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5612..5628)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5625..5647)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5636..5652
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5660..5666,5667..5684,5685..5690))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5705..5726
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5843..5860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6014..6602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6094..6113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6773..7564,7565..7633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7396..7415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7634..7738)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7765..8220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7852..7871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8062..8083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8346..8368
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8360..8377
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8361..8377
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8387..8405
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aaaatgatgt catggcttta gaagcttgtt gacattgatt attgactagt tattaatagt
       61 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      121 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      181 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      241 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      301 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      361 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      421 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      481 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      541 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      601 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
      661 atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga
      721 gctcggatcc catcgatgcc acaatgtcca acattatagt tgagcatcag tattctcata
      781 gactgaaatt gactgtggtg agagctgaaa atgtaactaa aggagcattt ggggatttat
      841 tggacacccc ggacccttat gttgagctgt cagtgccaac cacaccagag tcaagaaaac
      901 gaacccgcca cattaacaat gacattaacc ccaagtggaa cgagaccttt gagttcatat
      961 tggatcccaa tcaatccaat gttttggagg tgacactgat ggatgccaat tatgtcatgg
     1021 atgaaactct tggcacagca aaatattccc tctcaaaact caaagtggca caaatggagc
     1081 atgtgaccct ctctatcggc aagacaacca aagtgttcct cgacttgctc ttggaagttt
     1141 gcgcatccac agacctgcgc ttcagcatga cactgtgtga tcaagagaag ctgttcatgc
     1201 agacacgcag agacagggtc atgctcagca taaagaagct tcttaaaatg gaaaaccctc
     1261 gtttccttcc atcctcacca agagaggttc ccaccatcgc tattctgggc tctggaggag
     1321 gttttcgtgc aatggtgggc ttctctggtg tgatgaaggc tttgtatgaa tctggcgtgt
     1381 ttgactgtgc aacatatgtg gcaggacttt caggatcaac atggtatatg tctatgctgt
     1441 actctcatcc agagtttcct gctaaaggcc caggcgacat taataaagag ctcatgaaca
     1501 gagtcagtaa taacccactc aaactgctcc tgccccaaaa catcaaccgc tacgtcaaag
     1561 cactgtggaa gaagaaatct gctggacagc cggtcacctt cactgacatc ttcgggatgt
     1621 tgataggaga gacgcttatt ccagggagaa tgaacattaa gctgagctca ttgaaaggga
     1681 aaattaatga aggtcagagc cctcttcctc tcttcacctg tctgcacgtc aaacctgacg
     1741 tctctgagct catgtttgca gattgggtgg agttcagccc ttatgaaatt ggcatggcca
     1801 aatatggcac cttcatgtct cctgggctgt ttggcagcaa attcttcatg ggaagtgttg
     1861 tgaaacaata tgaggaaaac cccttacatt tcctgatggg tgtgtgggga agtgcttttt
     1921 ccatcctctt taaccgggtt ttgggtgtga aggagacgac cagcagtagc actatggagg
     1981 aggagctgga gcaaattaaa ccagagcaca ttgtgggtga cgattctgct gataacgagg
     2041 aggaaactca aagaggaggc acagagtctg cagatgcaga ggacgagcgc cagcgtcatg
     2101 cccaggccag ctgggtacag cgcatgttga cctccatcat gggcgacacc actctgttca
     2161 ccactcgtga gggccgcgca ggcaaagtgc acaacttcat gctgggactg aacctgaact
     2221 caaccctgcc cttctctccc ttcagcggca tcacacacca gacctcgctg gaggaggaag
     2281 tggacgctgt tactgatccg gatgagtttg agcgaatcta tgagcctctg gatgtgaaga
     2341 gcaagaaaat ccatgtggtg gacagtggct tgacctttaa cctgccctac cctctcattc
     2401 tgcggtgtca gagaggagtc gacctgatta tttcattcga cttctcagcc cgacccagtg
     2461 actccagccc accgttcaaa gagttactgc tggctgaaaa atgggcccgt atgaataagc
     2521 tgcccttccc gaagattgat tcgaaagtat tcgacagaga gggtctaaaa gagtgctatg
     2581 tgttcaagcc tgctaaagga gacaagaact gccccaccat cattcatttt gtcttggcca
     2641 acatcaactt ccggaatttt aaagcccctg gagttcctcg agattcagac aaggacatag
     2701 agtttggtga ctttgacata tttgacgagc cagcctcccc ttactccact ttcaacttca
     2761 aatacaacaa ccaggccttc aagagacttc atgacctcat ggagttcaat acgcttaaca
     2821 acatcgaggt tatcaaagaa gccattaaag acagcattct gctgagacga gagaacccag
     2881 cgcgatgttc ggtttccctt tcgctcagtg aaatcgaaaa caagaagttc ctgaaacgag
     2941 acaacagcat tgcaaaacga cccaccggag ctggaggaat tcgtcctgga tctatggtga
     3001 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3061 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3121 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3181 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3241 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3301 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3361 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3421 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3481 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     3541 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     3601 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     3661 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taactcgagc
     3721 ctctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3781 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3841 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3901 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3961 tggggatgcg gtgggctcta tggaccggac tgaggcggaa agaaccagct gtggaatgtg
     4021 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4081 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4141 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4201 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4261 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4321 ttttttggag gcctaggctt ttgcaaaaag ctagattttc tgaccctagc caccatgacc
     4381 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc
     4441 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac
     4501 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     4561 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     4621 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     4681 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     4741 tggttcctgg ccaccgtcgg cgtgtcgccc gaccaccagg gcaagggtct gggcagcgcc
     4801 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     4861 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     4921 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaggatct
     4981 cgagctcgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     5041 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     5101 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     5161 aagcaagtaa aacctctaca aatgtggtac ttaagaggga tcgataagga tctagcttgt
     5221 ggaaggctac tcgaaatgtt tgacccaagt taaacaattt aaaggcaatg ctaccaaata
     5281 ctaattgagt gtatgtaaac ttctgaccca ctgggaatgt gatgaaagaa ataaaagctg
     5341 aaatgaatca ttctctctac tattattctg atatttcaca ttcttaaaat aaagtggtga
     5401 tcctaactga cctaagacag ggaattttta ctaggattaa atgtcaggaa ttgtgaaaaa
     5461 gtgagtttaa atgtatttgg ctaaggtgta tgtaaacttc cgacttcaac tgtataggga
     5521 tcctctagct agagtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt
     5581 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5641 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5701 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5761 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5821 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5881 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5941 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6001 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     6061 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     6121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6181 tcccttcggg aaccgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6421 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6661 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6721 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6841 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6901 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6961 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7021 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     7081 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     7141 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     7201 ccttcggtcc tccgatcgtt gtcagaagta acttggccgc agtgttatca ctcatggtta
     7261 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7321 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7381 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7621 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7741 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggag
     7801 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7861 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7921 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7981 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8041 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8101 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8161 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     8221 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     8281 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     8341 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     8401 atagggcgaa ttggagctcg gatccctata cagttgaagt cggaagttta catacactta
     8461 agttggagtc attaaaactc gtttttcaac tactccacaa atttcttgtt aacaaacaat
     8521 agttttggca agtcagttag gacatctact ttgtgcatga cacaagtcat ttttccaaca
     8581 attgtttaca gacagattat ttcacttata attcactgta tcacaattcc agtgggtcag
     8641 aagtttacat acactaagtt gactgtgcct ttaaacagct tggaaaattc cag
//
