LOCUS       sequence-325043-        4425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF442 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF442
FEATURES             Location/Qualifiers
     source          1..4425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    26..125
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2007..2106)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2123..2142)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2124..2142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2265..2284)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2276..3085
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2343..2362)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3232..3820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3721..3740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3974..3991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4150..4177)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4269..4355)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4404..4425,1)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4418..4425,1..10)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(4419..4425,1..10)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg
       61 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagt
      121 tggcatgatc gtgttcggcg gcgaggacag atctgacctg ttcctgcccg acagccagac
      181 caacgaggag agaaagcagt acgacagcgt ggccttcgag gacgtggccg tgaactttac
      241 acaggaggag tgggccctgc tgggcccctc ccagaagagc ctgtacagag acgtgatgtg
      301 ggaaaccatc agaaacctgg actgcatcgg catgaagtgg gaggacacca acatcgagga
      361 ccagcacaga aaccccagaa ggagcctgag atgccacatc atcgagagat tcagcgagtc
      421 caggcagcca gatagcaccg tgaacgaaaa gccccccggc gtggacccct gcaaaagcag
      481 cgtgtgcggc gagattatgg gctgcagctt cctgaactgc tacatcacat tcgacgccgg
      541 ccacaagcct gacgagtgcc aggagtacgg ggaaaagcct cacacccaca agcaatgcgg
      601 caccgccttc aactaccacc acagcttcca gacccaggag aggccccaca caggcaagaa
      661 gagatacgac tgtaaggagt gtggaaagac cttctccagc agcggcaacc tgcggagaca
      721 tatcatcgtg cagagaggcg gcggccccta catctgcaag ctgtgcggaa aggcattctt
      781 ctggccctcc ctgttcagga tgcacgaaag gacacacacg ggagagaagc cctatgaatg
      841 taaacagtgc tgcaaagctt tccccatcta cagctcctac ctgagacacg agcgaactca
      901 caccggtgaa aagccatatg agtgcaaaca ctgctctaag gcctttcccg actacagcag
      961 ctacgtgagg cacgaacgca cccacacggg tgagaaaccg tacaaatgca agagatgcgg
     1021 cagagctttc agcgtgagca gctccctgag aatccacgag cgcacccata ccggcgagaa
     1081 gccatacgag tgcaagcagt gcggtaaagc ctttcaccac ctgggctctt tccagagaca
     1141 catgatcaga catacaggcg acggccctca caagtgcaag atctgtggga agggcttcga
     1201 ctgccccagc tcactgcagt cccacgagag aacccacact ggcgagaaac cttacgaatg
     1261 taagcagtgt ggcaaggctc tgagccacca ctcatccttc agatcccata tgatcatgca
     1321 caccggagac ggcccccata agtgcaaagt ctgcggcaaa gcattcatct accccagcgt
     1381 gttccagggc catgagagaa cacacacagg ggagaagcct tatgagtgta aggaatgcgg
     1441 taaggctttc agaatcagca gcagcctgag gagacatgag actacacaca ccggcgaaaa
     1501 accatacaag tgtaagtgcg gcaaggcctt catcgacttc tactccttcc agaaccacga
     1561 gacaacccat acaggagaaa agccctacga gtgcaaggag tgcgggaagg cttttagctg
     1621 cttcacctac cttagccagc accggaggac ccacatggca gagaaaccct acgaatgcaa
     1681 aacctgcaag aaggcattta gccacttcgg aaacctgaag gtgcacgagc ggattcacac
     1741 aggtgagaag ccgtatgaat gcaaggaatg taggaaagcc ttcagctggc tgacctgcct
     1801 gctgcggcac gagaggatcc acaccgggaa gaagtcttat gaatgccaac agtgcggaaa
     1861 agcatttacc agaagcagat tcctgagagg ccacgagaag acacataccg gggagaaaat
     1921 gcacgagtgt aaagaatgcg ggaaagccct gtccagcctg tctagcctgc acagacacaa
     1981 gcggacccac tggagagaca ccctgtaccc aactttcttg tacaaagttg gcattataag
     2041 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
     2101 tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca tagctgtttc
     2161 ttggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     2221 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     2281 ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata
     2341 tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta
     2401 tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga
     2461 tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc ttccgaccat
     2521 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa
     2581 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct
     2641 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga
     2701 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag
     2761 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa
     2821 acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct
     2881 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga
     2941 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca
     3001 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca
     3061 tttgatgctc gatgagtttt tctaatcaga attggttaat tggttgtaac actggcagag
     3121 cattacgctg acttgacggg acggcgcaag ctcatgacca aaatccctta acgtgagtta
     3181 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3361 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3601 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3781 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3841 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     3901 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     3961 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     4021 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     4081 gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg aagagtttgt
     4141 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt
     4201 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct
     4261 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
     4321 gcccagtctt ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
     4381 gttaacgcta gcatggatgt tttcccagtc acgacgttgt aaaac
//
