LOCUS       sequence-325045-        4878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ZNF749 transcription factor, part of the 
            Human TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ZNF749
FEATURES             Location/Qualifiers
     source          1..4878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2467..2566)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2583..2602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2584..2602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2725..2744)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2736..3545
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2803..2822)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3692..4280
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4181..4200
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4434..4451
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4610..4637)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4729..4815)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4864..4878,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4878,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catgaacctg accgaggact gcatggtgtt cgaggacgtg gccatctatt
      181 tcagccagga ggagtggggc attctgaacg acgcccagag acatctgcac agcaacgtca
      241 tgctggagaa ctttgccctg ctgagcagcg tgggctgctg gcacggcgcc aaggatgagg
      301 aagtgcccag caagcagtgt gtgagcgtga gagtgctcca ggtgacaatc cccaagcctg
      361 ccctgagcac cctcaaggcc cagccctgca agatgtgcag cagcatcctg aaggacatcc
      421 tgcacctggc cgaacacgac ggcacccatc ctgagcaggg cctgtataca tgcgccgccg
      481 agcacgacct gcaccagaag gagcagatca gggagaagct gaccaggagc gacgagtgga
      541 ggcctagctt cgtgaaccac agcgctcacg tgggagagag aaacttcacc tgtacccagg
      601 gcggaaagga cttcaccgcc tccagcgatc tgcttcagca gcaggtcctc aacagcggct
      661 ggaaactgta cagagacacc caggacggcg aggctttcca gggcgagcag aacgacttca
      721 acagcagcca gggaggcaaa gacttctgcc accagcacgg cctgttcgag catcagaaga
      781 cccacaacgg cgagcgccca tacgagttca gcgagtgcgg cgagctcttc aggtacaaca
      841 gcaacctcat caagtaccag cagaaccacg ccggggagag gccctacgaa ggcaccgagt
      901 acggcaagac cttcatcagg aagagcaacc tggtccagca ccaaaagatt cacagcgagg
      961 gcttcctgtc caagaggtcc gaccccatcg agcaccagga gatcctcagc agacccaccc
     1021 cttatgagtg tacacagtgc ggcaaggcat tccttaccca ggcccatctg gtgggccatc
     1081 agaaaaccca cacaggagag cagccgtacg agtgcaataa gtgtgggaag ttctttatgt
     1141 acaactccaa actgatcaga catcaaaaag tgcacacagg tgagagaagg tacgaatgtt
     1201 ctgagtgcgg gaagctgttt atggacagct tcaccctggg aagacatcag agggtgcaca
     1261 ccggtgagag gcctttcgaa tgctccatct gcggaaaatt cttcagccac agaagcaccc
     1321 tgaacatgca ccagcgggtg cacgccggaa agagactgta caagtgtagc gagtgtggta
     1381 aggcctttag cctgaagcac aacgtcgtgc agcatctgaa aatccacact ggcgagcggc
     1441 cctatgagtg cacagagtgc gagaaagctt ttgtgagaaa aagccacctc gtccagcatc
     1501 aaaagatcca cacagatgcc ttcagcaaga gatccgacct catccagcat aagagaatcg
     1561 acatcaggcc ccggccctac acctgttcag agtgtggcaa ggccttcctc acccaagccc
     1621 acctggtggg tcaccagaag atccataccg gagagaggcc atatgaatgc acccagtgtg
     1681 ccaaggcttt cgtgagaaag agccacttgg tgcagcacga aaaaatccat acagacgcct
     1741 tctccaagag aagcgacctg atccagcaca agagaattga cctgaggccc agaccttacg
     1801 tgtgcagcga atgcggcaaa gccttcctga cccaggctca tctggacggc caccagaaaa
     1861 ttcagaccgg cgagagaaga tacgaatgca atgagtgtgg aaagtttttc ctggactcct
     1921 ataagctggt gatccaccag cgcatccaca ccggagaaaa gccctataag tgcagcaagt
     1981 gcggaaagtt cttcagatac agatgcaccc tgtccagaca ccagaaagtt cacacaggcg
     2041 aaagacccta cgagtgctct gaatgcggta aatttttcag agacagctac aagctgatca
     2101 tccatcagag agtgcatacc ggcgaaaagc cttacgagtg tagcaactgc gggaaattcc
     2161 tgagatacag gagcaccttc attaagcacc acaaggtgtg tactggagag aagccccacg
     2221 agtgttccaa gtgcagagag ctgttcagga ccaagtctag cctgattatc caccaacaga
     2281 gccacacggg cgagagcccc ttcaagctga gggagtgcgg taaggacttt aacaagtgca
     2341 acaccggcca gaggcagaag acacatactg gcgaaaggag ctatgaatgt ggcgagtcca
     2401 gcaaggtgtt caagtacaat agcagcctga tcaagcacca gatcattcac accggcaaga
     2461 ggccctaccc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
     2521 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
     2581 tcccctatag tgagtcgtat tacatggtca tagctgtttc ttggcagctc tggcccgtgt
     2641 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     2701 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
     2761 cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg
     2821 ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag
     2881 agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca
     2941 gactaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc
     3001 ctgatgatgc atggttactc accactgcga tccccggaaa aacagcattc caggtattag
     3061 aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt
     3121 tgcattcgat tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc
     3181 aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta
     3241 atggctggcc tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg
     3301 attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat
     3361 taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca
     3421 tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat
     3481 atggtattga taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt
     3541 tctaatcaga attggttaat tggttgtaac actggcagag cattacgctg acttgacggg
     3601 acggcgcaag ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt
     3661 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3721 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3781 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3841 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3901 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3961 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4021 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4081 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4141 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4201 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4261 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4321 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4381 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     4441 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     4501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     4561 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
     4621 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
     4681 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
     4741 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
     4801 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
     4861 tttcccagtc acgacgtt
//
