LOCUS       sequence-325048-        1067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/
            Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 
            counterselection; aacC1 for gentamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Ellington AJ, Reisch CR
  TITLE     Efficient and iterative retron-mediated in vivo recombineering in 
            Escherichia coli.
  JOURNAL   Synth Biol (Oxf). 2022 May 3;7(1):ysac007. doi: 
            10.1093/synbio/ysac007. eCollection 2022.
  PUBMED    35673614
REFERENCE   2  (bases 1 to 1067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Synth Biol 
            (Oxf)."; date: "2022-05-3"; volume: "7(1):ysac007. doi"; pages: " 
            10.1093/synbio/ysac007. eCollection 2022"
COMMENT     Sequence Label: pTJV1ScGm-rpoB
FEATURES             Location/Qualifiers
     source          1..1067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(249..254,255..271,272..277)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             466..999
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
       61 agaggcggtt tgcgtattgg gcgcatgcat aaaaactgtt gtaattcatt aagcattctg
      121 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
      181 ttgtcgcctt gcgtataata tttgcccatg gacgcacacc gtggaaacgg atgaaggcac
      241 gaacccagtt gacataagcc tgttcggttc gtaaactgta atgcaagtag cgtatgcgct
      301 cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca gtggcggttt
      361 tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc aagcagcaag
      421 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagc
      481 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc aagtatgggc
      541 atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc tgctcttgat
      601 cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc ggactccgat
      661 tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt cgaccaagaa
      721 gcggttgttg gcgctctcgc ggcttacgtt ctgcccaggt ttgagcagcc gcgtagtgag
      781 atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat tgccaccgcg
      841 ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat ctacgtgcaa
      901 gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat acgggaagaa
      961 gtgatgcact ttgatatcga cccaagtacc gccacctaac aattcgttca agccgagatc
     1021 ggcttcccgg ccgcggagtt gttcggtaaa ttgtcacaac gccgcca
//
