LOCUS       sequence-325075-       10697 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10697)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtN26_:T35s
FEATURES             Location/Qualifiers
     source          1..10697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             126..1199
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      1265..1459
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1742..1846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1847..2641
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2812..3400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3301..3320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3523..3547
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      5800..6052
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7848..8022
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        8122..8301
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             8323..9117
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8377..8396)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8987..9006
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      9130..9382
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9477..9501
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9765..10394
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
       61 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
      121 tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg
      181 acctggtgga gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag
      241 cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac
      301 cgccggcagc cggtgcgtcg tcgattagga agccgcccaa gggcgacgag caaccagatt
      361 ttttcgttcc gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg
      421 ccgttttccg tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc
      481 cagacgggca cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg
      541 acctggtact gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga
      601 agggagacaa gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc
      661 ggcgagccga tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca
      721 ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat
      781 ccgagggtga agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg
      841 agtacatcga gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc
      901 cggacgtgct gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc
      961 tctaccgcct ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga
     1021 tctacgaacg cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc
     1081 tgatcgggtc aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc
     1141 cgatcctagt catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat
     1201 gtacggagca gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaagcttct
     1261 ttcctgtgga tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt
     1321 acattgggaa cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa
     1381 aagagaaaaa aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa
     1441 cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc
     1501 ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg
     1561 ctggccgctc aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg
     1621 cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac cgagctccca tccccccagg
     1681 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagcgctg gccaattcgt
     1741 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1801 acaataacct tgataaatgc ttcaataata ttgaaaaagg aagagtatgg ctaaaatgag
     1861 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgcaaaag atacggaagg
     1921 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
     1981 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
     2041 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
     2101 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
     2161 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
     2221 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
     2281 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagagg acactccatt
     2341 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
     2401 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
     2461 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
     2521 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
     2581 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
     2641 gctgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     2701 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     2761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     2821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     2881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     2941 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     3241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     3301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     3361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     3421 tttacggttc ctgtggttgg cacatacaaa tggacgaacg gataaacctt ttcacgccct
     3481 tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc gccaatatat
     3541 cctgtcaaac actgatagtt taaactccct cggtcacatg tgcatcctcg taaggaggac
     3601 gtcgttgtgg ttggtgcttt ccttacattc tgagcctctt tccttctaat ccactcatct
     3661 gcatcttctt gtgtccttac taatacctca ttggttccaa attccctccc tttaagcacc
     3721 agctcgtttc tgttcttcca cagcctccca agtatccaag ggactaaagc ctccacattc
     3781 ttcagatcag gatattcttg tttaagatgt tgaactctat ggaggtttgt atgaactgat
     3841 gatctaggac cggataagtt cccttcttca tagcgaactt attcaaagaa tgttttgtgt
     3901 atcattcttg ttacattgtt attaatgaaa aaatattatt ggtcattgga ctgaacacga
     3961 gtgttaaata tggaccaggc cccaaataag atccattgat atatgaatta aataacaaga
     4021 ataaatcgag tcaccaaacc acttgccttt tttaacgaga cttgttcacc aacttgatac
     4081 aaaagtcatt atcctatgca aatcaataat catacaaaaa tatccaataa cactaaaaaa
     4141 attaaaagaa atggataatt tcacaatatg ttatacgata aagaagttac ttttccaaga
     4201 aattcactga ttttataagc ccacttgcat tagataaatg gcaaaaaaaa acaaaaagga
     4261 aaagaaataa agcacgaaga attctagaaa atacgaaata cgcttcaatg cagtgggacc
     4321 cacggttcaa ttattgccaa ttttcagctc caccgtatat ttaaaaaata aaacgataat
     4381 gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac ggctggatct tatgacgacc
     4441 gttaaggaaa ttgtggttgt cggacgaagt ccagtaataa acggcgtcaa agtggttgca
     4501 gccggcacac acgagtcgtg tttatcaact caaagcacaa atacttttcc tcaacctaaa
     4561 aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt
     4621 ttattattag ctattgcttc accgccttag ctttctcgtg acctagtcgt cctcgtcttt
     4681 tcttcttctt cttctataaa acaataccca aagagctctt cttcttcaca attcagattt
     4741 caatttctca aaatcttaaa aactttctct caattctctc taccgtgatc aaggtaaatt
     4801 tctgtgttcc ttattctctc aaaatcttcg attttgtttt cgttcgatcc caatttcgta
     4861 tatgttcttt ggtttagatt ctgttaatct tagatcgtag acgattttct gggtttgatc
     4921 gttagatatc atcttaattc tcgattaggg tttcatagat atcatccgat ttgttcaaat
     4981 aatttgagtt ttgtcgaata attactcttc gatttgtgat ttctatctag atctggtgtt
     5041 agtttctagt ttgtgcgatc gaatttgtcg attaatctga gtttttctga ttaacagatg
     5101 cagatcttaa tggggtcatc caagaatgtt atcaaggagt tcatgaggtt taaggttcgc
     5161 atggaaggaa cggtcaatgg gcacgagttt gaaatagaag gcgaaggaga ggggaggcca
     5221 tacgaaggcc acaataccgt aaagcttaag gtaaccaagg ggggaccttt gccatttgct
     5281 tgggatattt tgtcaccaca atttcagtat ggaagcaagg tatatgtcaa gcaccctgcc
     5341 gacataccag actataaaaa gctgtcattt cctgaaggat ttaaatggga aagggtcatg
     5401 aactttgaag atggtggcgt cgttactgta acccaggatt ccagtttgca ggatggctgt
     5461 ttcatctaca aggtcaagtt cattggcgtg aactttcctt ccgatggacc tgttatgcaa
     5521 aagaaaacaa tgggctggga agccagcact gagcgtttgt atcctcgtga tggcgtgttg
     5581 aaaggagaga ttcataaggc tctgaagctg aaagacggtg gtcattacct agttgaattc
     5641 aaaagtattt acatggcaaa gaagcctgtg cagctaccag ggtactacta tgttgactcc
     5701 aaactggata taacaagcca caacgaagat tatacaatcg ttgagcagta tgaaagaacc
     5761 gagggacgcc accatctgtt cctttaagct tgtcaagcag atcgttcaaa catttggcaa
     5821 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     5881 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     5941 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     6001 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgacgctga
     6061 gctcgaattc tagtggaggg agagaggatt ttgaggaaat aattaattga attacttgta
     6121 ttattgataa agtaatttag ataagttgtt agtacaactt attgcaacta tggatagaca
     6181 aaaatcactt attttaaggg gagaagtata taacaactta ttataaaatt tctgagcagc
     6241 cggcctcctc caatcaatca tagagagtgg agcccattct ggaaaaccaa ggaaccccca
     6301 acttgcactg gtgcggtggc ccaattcaaa aaaaacgggg cccagcaaag taacgccgtt
     6361 gtaacgattt atcaatccaa actcaaaagg cggcgagatg cgtttaactc cgtgaaatta
     6421 acaaaccgcc aacaacttgc aatttgcaac taccgtttcc aagaagaact caaccacaca
     6481 acgtatccta tcccaaacca cacgcaacta gtgacgcgtc ataaggacac gtgtcacaat
     6541 ttgactggtt aataatttca ccgcttttgc tataaattac ctccaatccc cttagcttct
     6601 tcacaattca gttcccaacc ctaacaattc ttgttcatat cgcttctctc tactttcaag
     6661 gtatgatcca atttctctct tctttctctg taatcctttc gttgagtttt gtttccgatc
     6721 aatcataggt agttttcttg tttcgaagca tgagatctag gaattttttg tgattttcca
     6781 aaattgagat cggtttgaaa ttgaatttta cagcttgaat ctcagatctt gttttatcaa
     6841 tgttttcgat ggcttgcgat gtagatctat gataattgtg gttcagtttt gttaggaatc
     6901 gatttcggtt tagcaattgc agattaatta gggtttccaa ttgaattctt cagatccgtt
     6961 atggaattat gtcaaataat ttattcaaat tggaaattat tgttagatcc actcttaatc
     7021 tgtttgatcc aagcttcaat tagggttttc acttgtttca atttcttgtt atggattctg
     7081 atttatctgt tgatgttaga tccactctta atctgtttga tccaagcgtt aattagggtt
     7141 ttcacttgtt tcaatttcgt gtgttggatt ctgatttatc tgttgttgat gtgattacag
     7201 aatgggttct ttcaagctgt tgagtcttat agccatggtt ctcatgttac tattgcccat
     7261 ggctgccaaa ggggattttt ttgatgatgt ccttgaaaag gtttgtgaag aagtggagtg
     7321 tgggaaagga aactgtgtgg tgaattcaag ttaccctttg aactttgttt gtgaatgcga
     7381 ttctggctgg aagcgaaccc aagatgaaga tgatgatata tatgctacta gctttcttcc
     7441 atgtgtcatt cctcaatgta gcttgaatta tggttgccaa ccagcacctc ctccagttcc
     7501 agagaagact ttacctcgca acatttcagc ttttgatcct tgctattggg catattgtgg
     7561 agaaggacaa tgcaccaaga acaagacaca tacacacaga tgtgaatgca accccaatta
     7621 ttataatctt ctcaatatct ctgtcttccc ttgttacagt gaatgtactc ttggatctga
     7681 ttgttccaga cttggaatta aagtttcaaa ttcaaccgga gatgttgata gtcatgcaag
     7741 ctcaatcttt ggaggaaggt tccattggat cactgtgttg ttgatttcaa cgggtatggt
     7801 tatgtggagc taggcttctc tagctagagt cgatcgacaa gctcgagttt ctccataata
     7861 atgtgtgagt agttcccaga taagggaatt agggttccta tagggtttcg ctcatgtgtt
     7921 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat
     7981 ttctaattcc taaaaccaaa atccagtact aaaatccaga tcgctaagct tgaattcttg
     8041 cggagagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     8101 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     8161 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     8221 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     8281 attcccctcg gtatccaatt agagtctcat attcactctc aaatgattga acaagatgga
     8341 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
     8401 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
     8461 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tccaagacga ggcagcgcgg
     8521 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
     8581 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
     8641 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
     8701 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg aggacgtact
     8761 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
     8821 ccagccgaac tgttcgccag gctcaaggcg cggatgcccg acggcgagga tctcgtcgtg
     8881 acccagggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
     8941 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
     9001 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
     9061 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagct
     9121 tgtcaagcag atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     9181 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     9241 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     9301 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     9361 gtgtcatcta tgttactaga tcgacgctgg atccgaattc ggcagggccc actgcatcca
     9421 ccccagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     9481 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     9541 aaatcaccac tcgatacagg cagcccatca gccgtgcggc tgcatgaaat cctggccggt
     9601 ttgtctgatg ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc
     9661 cgccgtctaa aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg
     9721 tatatgatgc gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg
     9781 tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc
     9841 tgcaactcgc cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg
     9901 attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga
     9961 ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc
    10021 aggcggcgga cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc
    10081 agccaagccc ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca
    10141 ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca
    10201 cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt
    10261 cccgtatcac gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg
    10321 aatcagaacc cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat
    10381 caaaactcat ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag
    10441 tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac
    10501 gccagccatg aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat
    10561 gtacgcggta cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct
    10621 accagagtaa atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg
    10681 gcatggaaaa tcaagaa
//
