LOCUS       sequence-32508-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Guet CC, Bruneaux L, Min TL, Siegal-Gaskins D, Figueroa I, Emonet T,
            Cluzel P
  TITLE     Minimally invasive determination of mRNA concentration in single 
            living bacteria.
  JOURNAL   Nucleic Acids Res. 2008 Jul . 36(12):e73.
  PUBMED    18515347
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2008 Jul . 36(12):e73."
COMMENT     Sequence Label: pZS*12-MS2-GFP
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             406..417
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    459..475
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    482..498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(521..539)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        607..711
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctccagtgaa aagttcttct cctttacgca tggaacctcc accgccgtag atgccggagt
       61 ttgctgcgat tgctgaggga atcgggtttc catcttttag gagaccttgc attgccttaa
      121 caataagctc gcagtcggaa ttcgtggaga aaattggaat ggttagttcc atatttaagt
      181 acgaacgcca tgcgccttta ggcacctcga ctttgatggt gtatttgcga ttctgcgcag
      241 agctctgacg aacgctacag gttactttgt aagcctgtga acgcgagtta gagctgatcc
      301 attcagcgac cccgttagcg aagttgcttg gggcgacagt cacgtcgcca gttccgccat
      361 tgtcgacgag aacgaactga gtaaagttag aagccatgcg gtacctttct cctctttaat
      421 gaattcggtc agtgcgtcct gctgatgtgc tcagtatctt gttatccgct cacaatgtca
      481 attgttatcc gctcacaatt ctcgaggtga agacgaaagg gcctcgtgat acgcctattt
      541 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      601 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      661 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      721 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      781 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc ag
//
