LOCUS       sequence-32509-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Guet CC, Bruneaux L, Min TL, Siegal-Gaskins D, Figueroa I, Emonet T,
            Cluzel P
  TITLE     Minimally invasive determination of mRNA concentration in single 
            living bacteria.
  JOURNAL   Nucleic Acids Res. 2008 Jul . 36(12):e73.
  PUBMED    18515347
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2008 Jul . 36(12):e73."
COMMENT     Sequence Label: pZE31-DsRed-ms2x2
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..45
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     29..45
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_RNA        85..103
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        124..142
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     terminator      207..293
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(538..557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gccaccacct gttcctgtaa gcttccgctc tagaactagt ggatcccccg ggcagcttgc
       61 atgcctgcag gtcgactcta gaaaacatga ggatcaccca tgtctgcagg tcgactctag
      121 aaaacatgag gatcacccat gtctgcaggt cgactctaga ggatcggaat tcgatatcaa
      181 gcttaattag ctgagtctag aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      241 cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc
      301 cctagaccta gggcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      361 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      421 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      481 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      541 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      601 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      661 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      721 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      781 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      841 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
      901 cag
//
