LOCUS       sequence-325129-        8658 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of 
            mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8658)
  AUTHORS   Chai AWY, Yee PS, Price S, Yee SM, Lee HM, Tiong VK, Goncalves E, 
            Behan FM, Bateson J, Gilbert J, Tan AC, McDermott U, Garnett MJ, 
            Cheong SC
  TITLE     Genome-wide CRISPR screens of oral squamous cell carcinoma reveal 
            fitness genes in the Hippo pathway.
  JOURNAL   Elife. 2020 Sep 29;9. pii: 57761. doi: 10.7554/eLife.57761.
  PUBMED    32990596
REFERENCE   2  (bases 1 to 8658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-09-29"; volume: "9. pii: 57761. doi"; pages: " 
            10.7554/eLife.57761"
COMMENT     Sequence Label: pKLV2-U6gRNA5(hYAP1-Y1K)-PGKpuro2AmCherry-W
FEATURES             Location/Qualifiers
     source          1..8658
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..199
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     154..174
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             217..397
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    444..569
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1068..1301
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1486..1530
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1828..1945
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2020..2268
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2020..2040
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2191..2210
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2396..2895
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2431..2454)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2828..2847
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2935..3531
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2935..2954)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3431..3451
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3550..3603
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3604..4314
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3749..3767)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4374..4394)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4792..4803)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5255..5272)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5261..5485
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5511..5531)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5511..5529)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5550..5566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5550..5566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5563..5585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5574..5590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5598..5604,5605..5622,5623..5628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5643..5664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5781..5798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5952..6540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6032..6051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6711..7502,7503..7571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7334..7353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7572..7676)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7702..8157)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7839..7860)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8051..8070
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8284..8306
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8298..8315
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8299..8315
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8325..8344
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8325..8343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
       61 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      121 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      181 gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc
      241 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
      301 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
      361 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
      421 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
      481 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
      541 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
      601 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
      661 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
      721 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
      781 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
      841 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
      901 agcaagcggc cggccgcgct gatcttcaga cctggaggag gagatatgag ggacaattgg
      961 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1021 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1081 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1141 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1201 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1261 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1321 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1381 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1441 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1501 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1561 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1621 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     1681 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     1741 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
     1801 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     1861 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1921 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
     1981 gttagtaccg ggccctacgc gtccaaggtc gggcaggaag agggcctatt tcccatgatt
     2041 ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat taatttgact
     2101 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     2161 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     2221 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgc ccacagggag
     2281 gcgtcatgtt taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt
     2341 tatcaacttg aaaaagtggc accgagtcgg tgcttttttt ggatccaatt ctaccgggta
     2401 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     2461 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     2521 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     2581 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     2641 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     2701 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     2761 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     2821 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     2881 tctccgggcc tttcgacctg catccatcta gatctcgagc agctgaagct taccatgacc
     2941 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     3001 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     3061 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     3121 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     3181 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     3241 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     3301 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3361 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3421 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3481 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggcggcggg
     3541 tccggaggag agggcagagg aagtctccta acatgcggtg acgtggagga gaatcctggc
     3601 ccaatggtga gcaagggcga ggaggataac tccgccatca tcaaggagtt cctgcgcttc
     3661 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     3721 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     3781 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     3841 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     3901 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctctctgcag
     3961 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     4021 gtaatgcaga agaaaaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     4081 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     4141 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     4201 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     4261 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtgagcggcc
     4321 gctcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4381 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4441 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4501 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4561 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4621 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4681 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4741 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4801 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtcta
     4861 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca ggtaccttta
     4921 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4981 ctggaagggc taattcactc ccaaagaagt caagatctgc tttttgcctg tactgggtct
     5041 ctctggttag accagagtct ctctggttag accagatctg agcctgggag ctctctggct
     5101 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     5161 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     5221 ggaaaatctc tagcagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     5281 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     5341 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     5401 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagtcaa tagcaggcat
     5461 gctggggatg cggtgggctc tatgggcggc cgttaattaa gcttttgttc cctttagtga
     5521 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5581 ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc ctggggtgcc
     5641 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5701 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5761 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6661 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6721 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6781 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6841 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6901 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6961 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7021 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7081 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7141 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7201 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7261 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7321 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7381 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7441 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7501 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7561 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7621 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7681 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7741 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7801 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7861 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7921 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7981 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     8041 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     8101 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     8161 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     8221 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     8281 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac gactcactat
     8341 agggcgaatt gactagtaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     8401 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     8461 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     8521 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     8581 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     8641 tcatcgctat taccatgg
//
