LOCUS       sequence-32515-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  TITLE     Tono lab plasmid
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTitin
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(44..554)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(107..125)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    600..717
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtggcga ccggtggatc cccctgggga gagaggtcgg
       61 tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg
      121 aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg
      181 ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc
      241 atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga
      301 gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac
      361 ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg
      421 tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc
      481 cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc
      541 gcaaccccaa ccccgtggaa ttcgatatca agcttctcga ggctagtctc gtgatcgata
      601 aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc tgttgctatt
      661 atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct tttaaaagtt
      721 aaccgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
      781 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
      841 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agca
//
