LOCUS       sequence-32516-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     Tono lab plasmid
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTitin
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     413..430
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    547..568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(583..588,589..606,607..613)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    621..637
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     626..648
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     645..661
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     645..661
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     680..700
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        682..700
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgagctt
      121 ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattcg gcttttcagt
      181 catgctgcta gcgctgctgc tgctgaagct gctgaaggat gcctcctgct tagaggcaga
      241 catttggact gactgagacg agacgcttcc gtgcaagctc acgtcactgc tcgttttaag
      301 ccgaattctg cagatcatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      361 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      421 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      481 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      541 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      601 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      661 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
      721 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
      781 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      841 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      901 gaattgccgc attgca
//
