LOCUS       sequence-325161-        6294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tight affinity glutamate sensor ** A newer version of this sensor is
            available. Please see iGluSnFR3 plasmids at 
            https://www.addgene.org/browse/article/28220233/ **.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Marvin JS, Scholl B, Wilson DE, Podgorski K, Kazemipour A, Muller 
            JA, Schoch S, Quiroz FJU, Rebola N, Bao H, Little JP, Tkachuk AN, 
            Cai E, Hantman AW, Wang SS, DePiero VJ, Borghuis BG, Chapman ER, 
            Dietrich D, DiGregorio DA, Fitzpatrick D, Looger LL
  TITLE     Stability, affinity, and chromatic variants of the glutamate sensor 
            iGluSnFR.
  JOURNAL   Nat Methods. 2018 Nov;15(11):936-939. doi: 
            10.1038/s41592-018-0171-3. Epub 2018 Oct 30.
  PUBMED    30377363
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-018-0171-3"; journalName: "Nat Methods"; date: 
            "2018-11"; volume: "15"; issue: "11"; pages: "936-939"
COMMENT     Sequence Label: pAAV.CAG.SF-iGluSnFR.A184S
FEATURES             Location/Qualifiers
     source          1..6294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   458..587
                     /label=AAV2 ITR
     rep_origin      659..1114
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(746..765)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     956..977
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1140..1244
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1245..1313,1314..2105)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1463..1482)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2276..2864
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2765..2784
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3370..3491
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3407..3426)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3461..3480
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    3534..4122
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3640..3651
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4049..4069
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4138..4284)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(4288..4317)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     4782..4803
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4906..4927)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     sig_peptide     complement(5896..5955)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     regulatory      5952..5961
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5995..6014)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(6076..6098)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccccgcctgg cgcgcgcccc cccccccccc cccccccccc cgcccccatc gctgcacaaa
       61 ataattaaaa aataaataaa tacaaaattg ggggtgggga ggggggggag atggggagag
      121 tgaagcagaa cgtggggctc acctcgacca tggtaatagc gatgactaat acgtagatgt
      181 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
      241 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
      301 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
      361 ttactatggg aacatacgtc attattgacg tcaatggtac tcttcctaga gcatggctac
      421 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
      481 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
      541 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg gcgtaatagc
      601 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
      661 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      721 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      781 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      841 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      901 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      961 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     1021 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     1081 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     1141 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1201 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1261 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1321 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1381 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1441 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1501 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1561 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1621 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1681 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1741 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1801 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1861 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1921 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1981 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     2041 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     2101 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     2161 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2221 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2281 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2341 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2401 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     2461 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2521 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2581 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2641 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2701 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2761 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2821 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2881 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2941 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3001 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     3061 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgcgcgctc gctcgctcac
     3121 tgaggccgcc cgggcaaagc ccgggcggcc tcagtgagcg agcgagcgcg cagagaggga
     3181 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
     3241 tctacgtagc catgctctag gaagatcctt atcgatttta ccacatttgt agaggtttta
     3301 cttgctttaa aaaacctccc acatctcccc ctgaacctga aacataaaat gaatgcaatt
     3361 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3421 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3481 aatgtatctt atcatgtctg ctcgaagcgg ccgcccgggt cgacggtatc gatgcgggga
     3541 ggcggcccaa agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg
     3601 cagagccggc agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta
     3661 gcagaaggac gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga
     3721 cgatgatttc cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca
     3781 gcagcgggca aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa
     3841 agtcccggaa aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc
     3901 aaacacagtg cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga
     3961 cagcaaccag gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg
     4021 atacaaaggc attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat
     4081 accagtcaat ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttctaacg
     4141 tggcttcttc tgccaaagca tgatgaggat gataagggag atgatggtga gcaccaccag
     4201 ggccaggatg gctgagatca ccaccacctt aaagggcaag gagtgtggca ccacgatgac
     4261 ctcctgcgtg tcctggccca cagcattcag atcctcttct gagatgagtt tttgttcgtc
     4321 gacctgcagt ttcagtgcct tgtcattcgg ttctttgaac agtgctttca tttcgtctga
     4381 cagttcgaaa ttcatgttca gggggttgtt aaagttgtac tccagcttgt gccccaggat
     4441 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     4501 ctcgaacttc acctcggcgc gggtcttgta ggtgccgtcg tccttgaagc tgatggtgcg
     4561 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     4621 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     4681 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt tggtggcatc
     4741 gccctcgccc tcgccgcgca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     4801 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcatgctcc ctccggtacc
     4861 gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag
     4921 gaccatgtga tcgcgcttct cgttggggtc tttgctcagc acggactggg tgctcaggta
     4981 gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc
     5041 ggcgagctgc acgctgccgt cctccacgtt gtggcggatc ttgaagttcg ccttgatgcc
     5101 gttcttctgc ttgtcggcgg tgatatagac gttgtggctc accagaattg gatttttgaa
     5161 ccacttatca aaccattttt ccgcttcacc ggaggtctgc acctgagcga tggtgtcatc
     5221 catcagcttt ttgaactgcg gatcatcttt acgcaacata caaccgtagg cctcctgaga
     5281 ctgcggcttg ccgacgattt cccagttgtc tggtttcttc gctttcgcac gttcaccggc
     5341 cagcaggctg tcatccatca taaaggcaac ggcacgaccg ctttccaggg tgcggaaaga
     5401 gtcaccgtga tctttggcgc tgatgatgcg catattcatt ttttgctctt cattcagttt
     5461 gttgagcaaa acttcagagg tagtgccgga agtgacgact acggctttgt ctttcaggtt
     5521 ggcaaaatct ttgatatcgc cacccttttt ggtcaacagg cgcgtaccga ccacgaaaat
     5581 agtgtcagag aaagccgcct gtttttggcg ttcgacgttg ttggtggtag aaccacattc
     5641 aaaatcgaaa gtgccgtttt gcagcagtgg aatacggttt tgtgaggtaa tcggaatcag
     5701 ttttacctgc aagtccggtt tgttgagttt ctttttcact gcttcaacaa tggcgttgga
     5761 gtaatcctgc gagtaaccca ccactttttg ctgattgtcg taataagaga aaggcactga
     5821 agattcacgg tgaccgacga caatcacacc gtttttggcg attttgtcca gcgtgctgcc
     5881 tgcggcagat ctgtcaccag tggaacctgg aacccagagc agcagtaccc atagcaggag
     5941 tgtgtctgtc tccatggtgg cgggatccaa ttctttgcca aaatgatgag acagcacaat
     6001 aaccagcacg ttgcccagga gctgtaggaa aaagaagaag gcatgaacat ggttagcaga
     6061 ggctctagag ccgccggtca cacgccagaa gccgaacccc gccctgcccc gtcccccccg
     6121 aaggcagccg tccccccgcg gacagccccg agccgctccc cccgcacaaa gggccctccc
     6181 ggagcccctc aaggctttca cgcagccaca gaaaagaaac aagccgtcat taaaccaagc
     6241 gctaattaca gcccggagga gaagggccgt cccgcccgct cacctgtggg agta
//
