LOCUS       sequence-325165-        8659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of 
            mCherry tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Chai AWY, Yee PS, Price S, Yee SM, Lee HM, Tiong VK, Goncalves E, 
            Behan FM, Bateson J, Gilbert J, Tan AC, McDermott U, Garnett MJ, 
            Cheong SC
  TITLE     Genome-wide CRISPR screens of oral squamous cell carcinoma reveal 
            fitness genes in the Hippo pathway.
  JOURNAL   Elife. 2020 Sep 29;9. pii: 57761. doi: 10.7554/eLife.57761.
  PUBMED    32990596
REFERENCE   2  (bases 1 to 8659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-09-29"; volume: "9. pii: 57761. doi"; pages: " 
            10.7554/eLife.57761"
COMMENT     Sequence Label: pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W
FEATURES             Location/Qualifiers
     source          1..8659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        279..477
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     432..452
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             495..675
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    722..847
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1346..1579
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1764..1808
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2106..2223
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2298..2546
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2298..2318
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2469..2488
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        2675..3174
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2710..2733)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3107..3126
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3214..3810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3214..3233)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3710..3730
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3829..3882
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3883..4593
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNSAIIKEFLRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4028..4046)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(4653..4673)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5071..5082)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5534..5551)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5540..5764
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5790..5810)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5790..5808)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5829..5845)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5829..5845)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5842..5864)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5853..5869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5877..5883,5884..5901,5902..5907))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5922..5943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6060..6077)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6231..6819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6311..6330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6990..7781,7782..7850))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7613..7632
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7851..7955)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7981..8436)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8118..8139)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8330..8349
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8563..8585
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8577..8594
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8578..8594
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8604..8623
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8604..8622
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
       61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      241 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      301 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      361 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      421 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagcgc
      481 gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
      541 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
      601 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
      661 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
      721 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
      781 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
      841 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
      901 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
      961 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     1021 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     1081 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     1141 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgcgctga
     1201 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1261 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1321 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1381 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1441 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1501 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1561 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1621 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     1681 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     1741 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     1801 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     1861 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     1921 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     1981 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2041 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2101 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2161 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2221 tttcgggttt attacaggga cagcagagat ccagtttggt tagtaccggg ccctacgcgt
     2281 ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat
     2341 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     2401 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     2461 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     2521 atatcttgtg gaaaggacga aacaccgtgc acgatctgat gcccgggttt aagagctatg
     2581 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     2641 ccgagtcggt gctttttttg gatccaattc taccgggtag gggaggcgct tttcccaagg
     2701 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
     2761 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
     2821 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
     2881 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
     2941 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
     3001 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
     3061 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
     3121 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
     3181 atccatctag atctcgagca gctgaagctt accatgaccg agtacaagcc cacggtgcgc
     3241 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     3301 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     3361 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     3421 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     3481 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     3541 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     3601 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     3661 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     3721 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     3781 acctggtgca tgacccgcaa gcccggtgcc ggcggcgggt ccggaggaga gggcagagga
     3841 agtctcctaa catgcggtga cgtggaggag aatcctggcc caatggtgag caagggcgag
     3901 gaggataact ccgccatcat caaggagttc ctgcgcttca aggtgcacat ggagggctcc
     3961 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     4021 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     4081 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     4141 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     4201 ggcggcgtgg tgaccgtgac ccaggactcc tctctgcagg acggcgagtt catctacaag
     4261 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaaaaccatg
     4321 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     4381 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     4441 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     4501 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     4561 tccaccggcg gcatggacga gctgtacaag tgagcggccg ctcaacctct ggattacaaa
     4621 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4681 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4741 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4801 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4861 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4921 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4981 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     5041 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5101 cgcggcctgc tgccggctct gcggcctctt ccgcgtctac gccttcgccc tcagacgagt
     5161 cggatctccc tttgggccgc ctccccgcag gtacctttaa gaccaatgac ttacaaggca
     5221 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     5281 caaagaagtc aagatctgct ttttgcctgt actgggtctc tctggttaga ccagagtctc
     5341 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5401 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5461 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtttaa
     5521 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     5581 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     5641 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     5701 gacagcaagg gggaggattg ggaagtcaat agcaggcatg ctggggatgc ggtgggctct
     5761 atgggcggcc gttaattaag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     5821 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5881 atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5941 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6001 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     6061 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6121 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     6541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     6601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6661 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6901 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6961 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7021 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7081 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7141 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7201 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7261 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7321 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7381 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7441 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7501 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7561 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7621 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7681 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7741 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7801 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7861 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7921 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     8041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     8101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     8161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     8221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     8341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     8461 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     8521 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     8581 aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg actagtacat
     8641 aacttacggt aaatggccc
//
