LOCUS       sequence-325185-       12731 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration of a DiCre cassette in PbGFP parasite genome.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12731)
  AUTHORS   Fernandes P, Briquet S, Patarot D, Loubens M, Hoareau-Coudert B, 
            Silvie O
  TITLE     The dimerisable Cre recombinase allows conditional genome editing in
            the mosquito stages of Plasmodium berghei.
  JOURNAL   PLoS One. 2020 Oct 12;15(10):e0236616. doi: 
            10.1371/journal.pone.0236616. eCollection 2020.
  PUBMED    33044964
REFERENCE   2  (bases 1 to 12731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-10-12"; volume: "15(10):e0236616. doi"; pages: " 
            10.1371/journal.pone.0236616. eCollection 2020"
COMMENT     Sequence Label: pDiCre-mCherry
FEATURES             Location/Qualifiers
     source          1..12731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(18..36)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(57..73)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(57..73)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(70..92)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    81..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(105..111,112..129,130..135))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    150..171
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(288..305)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(459..1047)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(539..558)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1218..2009,2010..2078))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1841..1860
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2079..2183)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2209..2664)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2346..2367)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2558..2577
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2791..2813
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2805..2822
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2806..2822
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2829..2848
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2829..2847
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3719..4426
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3861..3879)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4129..4148
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(5633..5953)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(5960..5980)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7057..7077
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7084..7359
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRIS"
     protein_bind    9074..9107
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    12024..12057
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggtaccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca
       61 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      121 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      181 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      241 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      301 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      361 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      421 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      481 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      541 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      601 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      661 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      721 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      781 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      841 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
      901 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
      961 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1021 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1081 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1141 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1201 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1261 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1321 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1381 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1441 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1501 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1561 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1621 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1681 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1741 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1801 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1861 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     1921 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     1981 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2041 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2101 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2161 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     2221 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2281 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     2341 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2401 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     2461 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2521 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     2581 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     2641 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     2701 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     2761 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
     2821 gtgaattgta atacgactca ctatagggcg aattggagct ccaccgcgga ggtatatata
     2881 atagctgctt taactaaggg gctaataaat ggccccgtat tttgtttttt tttatagcta
     2941 ataataaaaa taaaaaaata aaaagtacga actttagtcc cataaattaa gaagtatata
     3001 tgcttataaa aaggatatca aataaattaa tgttaactca aataaattaa ttttcatgca
     3061 aaaaaatatt taaaaaagaa tatatatatt aagaaaaata attataaata ttatttacat
     3121 atatatacca taatatatac ccccttttga ttatatatat ataatattat atatttttct
     3181 ttagagaaaa aataaatagt gttatttcct attatatatc gtagttaatt ttaataattt
     3241 acaatacact atatttctta ttttatataa aatatattta taattaattt ttttatatta
     3301 tatgtatata ttttaattgc attctccttt tttaataaaa attttaccca attgttatag
     3361 agcttgttat tattatataa taacaatata agaatatttt ttatataaca ttaaaaaaaa
     3421 ttaacatgtt ttttattttt tttaaatatt tattccaatt tatttttaac ggtcaaatta
     3481 aagaaatata tagatatata tttattgctt aaattataag tatatgtttt tttaaaaaat
     3541 taaagcccaa aacatatttc tttgtattat atacattttt gcattttaca ctattttgcc
     3601 ataagcacat taaatatata tatatatgct tgtagcaaat taattaaata aaataattac
     3661 caacattatt atcccaataa attacaatta caaaatgagc ggccgctcta gaactagtgt
     3721 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3781 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3841 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3901 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3961 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4021 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4081 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4141 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4201 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4261 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4321 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4381 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaggat cccgtttttc
     4441 ttacttatat atttatacca attgattgta tttataactg taaaaatgtg tatgttgtgt
     4501 gcatattttt ttttgtgcat gcacatgcat gtaaatagct aaaattatga acattttatt
     4561 ttttgttcag aaaaaaaaaa ctttacacac ataaaatggc tagtatgaat agccatattt
     4621 tatataaatt aaatcctatg aatttatgac catattaaaa atttagatat ttatggaaca
     4681 taatatgttt gaaacaataa gacaaaatta ttattattat tattattttt actgttataa
     4741 ttatgttgtc tcttcaatga ttcataaata gttggacttg accgcggtgg cggccgccct
     4801 cagccgtacg ggatccagaa ttcggaactt aataaaaaag aggagtaatg tacgctcaaa
     4861 taataatgtt aaatggactt ataaatattg tcttttaaaa atgtgcctta catttattta
     4921 ttattattat tatattttaa ttatattttt attatatttc atttatttat ttatttattt
     4981 tttttttttt tttggtaaaa atataacatt ttatgcttct taaaaattgg caggttaaaa
     5041 aaatataaac atttatgtta tttctctttt gtgtgacatt tatgtgtaaa tggaacaaaa
     5101 attgtatttt tcatatatat aatataaata aataaaacca taaaccgttt atattttgtt
     5161 cctgcattta agaaaattta gaattatttc agttaaaaca agataaaaaa aattatctac
     5221 taataaataa ataaataaat aaatatatat atatatatat taaaaataca taagaaaaaa
     5281 aaaaaatgat aataaatata aaaaaaataa taataatata gaaaaagtta catgcacgta
     5341 tatttatacg aatgtatata taatgcatat ataaaatata cgtctcaaat atatatatat
     5401 atatatacat ataaacgtat gatatttact tatttatttt attctgcata tttaaaaatc
     5461 ctgcagttaa tcagttcagc ttgcaccagg cagcccagga tctgcacaca gacaggagca
     5521 tcttccaggt gtgttcagag aaggcctgcc tgtccctgaa catgtccatc aggttcttcc
     5581 tgacttcatc agaggtggat ccgttgctgg cgccggggtt gctggggcta gcttccagtt
     5641 ttagaagctc cacatcgaag acgagagtgg catgtggtgg gatgatgcct gggtgcccag
     5701 tggcaccata ggcataatct ggagatatag tcagtttggc tctctgaccc acactcatct
     5761 gggcaacccc ttcttcccag cctcggatca cctcctgctt gcctagcata aacttaaagg
     5821 gcttgtttct gtcccgggag gaatcaaatt tctttccatc ttcaagcatc ccggtgtagt
     5881 gcaccacgca ggtctggccg cgcttgggga aggtgcgccc gtctcctggg gagatggttt
     5941 ccacctgcac tcctctagac acctttctct tcttcttagg ggccattttc atatgtataa
     6001 aatttttatt tatttataag caaatatata tttttatata tttatacaca aagtatatat
     6061 attttaggaa atattaaaat aaatttaaat tttgaaggca aatgaaaaaa ttacgttttt
     6121 tgtttcacaa caattatatt tattttttga attaatttac atattatttt atattaaaat
     6181 tataaaataa aaaaaacatt acatatatta attatattat tatatgagga tttttttata
     6241 acatagaaat ataaataatt acgactagtt aataaagggc actttaattt ttatgcataa
     6301 ttagcttata tttagtgcat atttattttt atacatattt ttatatattt tttgatttac
     6361 atggcgtttt atgtttatat gtataacatt atatgcatag tatttttttg aaaatttaat
     6421 ggtatttcag tggatgaaaa tattactggt gctttgaggg gtgagcattt aaagcacaat
     6481 atctaggata ctacaaaaaa tacttaaagt atgaatatta aattgtaaac ttaagcataa
     6541 agagctcgaa aagaattaag cttaactcat ctacaagcat caaattaaat gttttatgca
     6601 gtaccttttt ttaaataatc cattttattt ttttatactc cgaggatttt acacctgcac
     6661 tgacactaca aatatagtaa tattttatcc ctacattata tgcattttat aatattttta
     6721 tttattttaa taatttatac acataagtac atagtataga gttgaaaaaa aaatacgtgt
     6781 atttctaaaa taaaatgaaa taaatattat gttattatat tgaatacaat aaacattttc
     6841 tccaaattaa aatttaatat tatataacaa aaaaaaatac aaaacaccaa ttaaaaataa
     6901 taataatttt atccttattt tttaataact gccattttta aagtatataa taaaaaataa
     6961 attaaatttt aaaaatattt caaaatataa aataaatata taagaatata tatttgctta
     7021 taaataaata aaaattttat acatatgaaa atggccccta agaagaagag aaaggtgtct
     7081 agaatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
     7141 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
     7201 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
     7261 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa
     7321 gcctgggacc tctattatca tgtgttccga cgaatctcag ctagccccag caaccccggc
     7381 gccagcaacg gatccaacag gaaatggttc cctgctgaac ctgaggatgt gagggactac
     7441 ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca cctgggccag
     7501 ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa tgctgtgtcc
     7561 ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc caagcaggcc
     7621 ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa ctctgacaga
     7681 tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct gctgcgcatt
     7741 gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg gagaatgctg
     7801 atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa ggccctgtcc
     7861 ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc tgatgacccc
     7921 aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc tgccacctcc
     7981 caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct gatctatggt
     8041 gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc cagagtgggt
     8101 gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca ggctggtggc
     8161 tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga gactggggcc
     8221 atggtgaggc tgctggagga tggggactga gaattctgta ctgttaattt taaaatgtgg
     8281 aatgccacga acatattttt tcgaacaaaa tatatatttt ttgtgtcgaa atacataaat
     8341 ttgtgtacct cctttttgtt gtatatatga aaaaggaata aagtgaatgg agtcgaatta
     8401 ctctagaccc tacaacttgt atatcactac tttataatac tataatatta ttattaacat
     8461 taaactatag aagaaatatc aacaatactt atgtatttgt tcaaacttta tcagtatagt
     8521 ttggtaatcg catatcatat atttgttatg tgtaatattt tatgaatatg cgtctttttt
     8581 tttaactata aataattcaa aataaaataa cataattaac atttgcaagc ataattaaga
     8641 tgattacttt cacatttcat ccaaagtgga aaatgaccac cacatttgat cgaagctttt
     8701 accacttttg atgcattagc tctgaaatgt atacaagctt tcatttgtta atgttacaaa
     8761 gggaagtttt tatttggggg cacattaaaa gtgaaaaaat taaaagttaa aaaagttaaa
     8821 aaatgatcgg tgtagttggt catatagaca tgttgttatg caagtctttc aaaacgttcc
     8881 tcataaaata aaaggaggga tctagagagc tttcaattct ccaaacggta ttattggtat
     8941 tttctgaaga ctgcgtttcc ttgtttttat ttatatttat gagatgttct ttttttatac
     9001 tcgcttttcc attaagtatg atttgatttt cattgacaaa attgagacaa acattaggga
     9061 atctatttat actataactt cgtataatgt atgctatacg aagttatcaa ttcgatatcg
     9121 aaattgaagg aaaaaacatc atttgtgttt atatgttttt ttttaatttt tcaacttttt
     9181 ttatttaact atgctaatca tattataaac attttaaaaa tcaagtccaa ctatttatga
     9241 atcattgaag agacaacata attataacag taaaaataat aataataata ataattttgt
     9301 cttattgttt caaacatatt atgttccata aatatctaaa tttttaatat ggtcataaat
     9361 tcataggatt taatttatat aaaatatggc tattcatact agccatttta tgtgtgtaaa
     9421 gttttttttt tctgaacaaa aaataaaatg ttcataattt tagctattta catgcatgtg
     9481 catgcacaaa aaaaaatatg cacacaacat acacattttt acagttataa atacaatcaa
     9541 ttggtataaa tatataagta agaaaaacgg gatcaaagct agcttctgta tttccgctag
     9601 acagccatct ccatctggat tcgtccgtgc gggacgtagc ccacgacctc aaaatcctcg
     9661 gcggtgaaat cgtcgatttc cttgatgcgt tccttgttga ggatgttcac aatggggaac
     9721 ggtctcggtt ctctccgcag ctgctctttt aaagcctcga catggttcgt gtagacatgc
     9781 gtgttcccca tgaagtgaat gaactcctta ggttttaggt tgcagacgtg tgcaaccatg
     9841 agcgtcaaaa gcgaatagga agcgatgttg aaggggacgc cgaggccgac atcgcacgac
     9901 cgctgataca tgatgcacga cagctccttc tggtcgttca cgtagaactg gcacaacaag
     9961 tgacaaggcg gcagcgccat ttcgtccagc gctgcaggat tccaggcagt catgagcatg
    10021 cgacgatctg ttggattcgt tctcagcatc tggatcacat tcttcagctg gtcgacgccc
    10081 tgccctgtgt agtctgtgtg catgtctttg tatgccgcgc cgaagtgtct ccactggaag
    10141 ccgtagcccg ggccgatgtc tccgacctct cggtggggga gattgcgcga atcgaggaac
    10201 tcgcgtgtca cattcttgtc ccagatcttc acgcccttct cagaaagatg gtttgcgttc
    10261 gtgtcgccgc gaatgaacca cagcaactct tcgaggaccc ctttccagaa cacacgcttt
    10321 gtggtgagaa gtggaaaggc ctgatccagc gagtagcgca tagtgcagcc gaatttggag
    10381 atgacaccaa cgcccgttcg gtcatccatt gtccttccat tgttaataat gtcggcaatg
    10441 agatcaaggt actggaactc ttcatggcct ctaaagtgaa catgcggaac ggcccgaatc
    10501 agttcctttt gctcgcgttt tttccggtct tcttcgtcca tccacgccaa caccggggca
    10561 atggctgcgg ccgaagaagg agcctgcaac ccgtgcacgg gagttgtctc cctcgtggac
    10621 gtcaaggagc tcattgcgtt gctcggttcc gcagtggctg cgtcgtcagt cttccttctc
    10681 ttctcgagaa ccacaaagtc gtagggaacc ccgttgtctg agaaggtctt ggaaatgaag
    10741 atgggtcgat acgtcgcttc attgtccttc tctcttccga gctccggaca aaagggaacg
    10801 aacacagact cggcaggagc tgcagcctgc gcagcagttg atttgtttga aagaatgtca
    10861 tctccgggga acgcagggaa gaaaacgtcg cacggaaact cgcgggctac acgcgtgatg
    10921 tacaggtgag aggcaacgcc cagagacagc gctgcctcgt acagtcccgc tcctcccacg
    10981 acaaaaatct ggtcgacaga atccttgtac tcttcctcca gaaggctgag agctgctggg
    11041 agtgaagcac agactcggac gcgctgctgg ccttcagctt gaggcttctc cgccgcaatg
    11101 tcttcttctt tgagggaaga ggaaacgacg atgttcaatc tgtccacgag gggtctaaac
    11161 tttcgaggca tgctttccca gtttttccgt cccatgacaa cggcgttgaa tctcttgccg
    11221 actgatggag agggaagtcc agagtcgccc gtctttgcaa atttcctggg aagccacccg
    11281 ttcaggcgac tggcttcttc gggcgtcgtt tttgtcacac gacgaaagtg tttgaaatct
    11341 gtggtcaagt ggggccacgg gaggccgttg ttgatgccga tgcccctctt gggggtcatc
    11401 gcgacgacca gacacaccgg tttacgcatt tttataaaat ttttatttat ttataagcaa
    11461 atatatattt ttatatattt atacacaaag tatatatatt ttaggaaata ttaaaataaa
    11521 tttaaatttt gaaggcaaat gaaaaaatta cgttttttgt ttcacaacaa ttatatttat
    11581 tttttgaatt aatttacata ttattttata ttaaaattat aaaataaaaa aaacattaca
    11641 tatattaatt atattattat atgaggattt ttttataaca tagaaatata aataattacg
    11701 actagttaat aaagggcact ttaattttta tgcataatta gcttatattt agtgcatatt
    11761 tatttttata catattttta tatatttttt gatttacatg gcgttttatg tttatatgta
    11821 taacattata tgcatagtat ttttttgaaa atttaatggt atttcagtgg atgaaaatat
    11881 tactggtgct ttgaggggtg agcatttaaa gcacaatatc taggatacta caaaaaatac
    11941 ttaaagtatg aatattaaat tgtaaactta agcataaaga gctcgaaaag aattaagctt
    12001 aactcatcta taagcatcgt cgaataactt cgtataatgt atgctatacg aagttatgga
    12061 tccgcgatcg cggcgcgccg gtaccgtctc ttcaatgatt cataaatagt tggacttgat
    12121 ttttaaaatg tttataatat gattagcata gttaaataaa aaaagttgaa aaattaaaaa
    12181 aaaacatata aacacaaatg atgttttttc cttcaatttc gatatcatat agcaatcgta
    12241 ttagcaggta aatgagcaag gttaataaaa aaatggtaaa aaaaaaaaaa ttggggaaaa
    12301 ataaacaagg tgttctttat ctacactttg gctttacaat gtataattta tatgcataca
    12361 aaaaaatttt gataaaattg taaaaaggtg aataaaataa cattgtaaca gtagtagata
    12421 gtaaagggaa ggtgttgctc aaatagtgtc gaaacaaaac tggcataaag aaaaattgat
    12481 attgagcaga ggatatgcgc ataatggtat tttgtttgtt tgtttgtttt ctcatttttt
    12541 gagacagctc aattctttat gtccacaaca tcatcggact tttcttcttc agggtagaaa
    12601 tattcatcct acaaaaaaaa aaaaaaatta ttttgaaaat ttatcataga tggttataat
    12661 aataacttat aaatatgaga tatatatata tataatttgt gagggatatg tattttattt
    12721 tattaatttc a
//
