LOCUS       sequence-325186-        8458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of H. volcanii codon-optimized Dendra2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8458)
  AUTHORS   Turkowyd B, Schreiber S, Wortz J, Segal ES, Mevarech M, Duggin IG, 
            Marchfelder A, Endesfelder U
  TITLE     Establishing Live-Cell Single-Molecule Localization Microscopy 
            Imaging and Single-Particle Tracking in the Archaeon Haloferax 
            volcanii.
  JOURNAL   Front Microbiol. 2020 Nov 20;11:583010. doi: 
            10.3389/fmicb.2020.583010. eCollection 2020.
  PUBMED    33329447
REFERENCE   2  (bases 1 to 8458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2020-11-20"; volume: "11:583010. doi"; pages: " 
            10.3389/fmicb.2020.583010. eCollection 2020"
COMMENT     Sequence Label: pTA231-p.Syn-Dendra2Hfx
FEATURES             Location/Qualifiers
     source          1..8458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1072..1430)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1112..1133)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1324..1343
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1557..1579
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1571..1588
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1572..1588
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1598..1617
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1598..1616
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1641..1657
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1642..1658
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2522..2542)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2522..2540)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2561..2577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2561..2577)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2574..2596)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2585..2601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2609..2615,2616..2633,2634..2639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2654..2675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2792..2809)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6711..7299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6791..6810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7470..8261,8262..8330))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8093..8112
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8331..8435)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttac tagagcagtt atgtgcgttc
      121 cggatgcggt gggcgttgcg acgcgcccgc taccgcaccg tcttcgagtt cgcgggcgag
      181 cgtttcgagt cggtccgcga ccgtttcggc gtcgtcgccg ttctcgtggc cctcggcgac
      241 gatgtcgacg agcgcgctgc cgacgatgat gccgtcggca ccgctggcga cgatgcgctc
      301 ggcgtggtcg ccgtcgctga ttccgaagcc gaccgccttg ggcacgtcgt agtcggtgag
      361 tcgttcgagc gacgagtcgg tctggtcgga gacgctcgac tgcgcgcccg tcgtgccgag
      421 gcgcgcctgc acgtagacgt agcccgagac ctgttccatg attcggtcga ggcgctcgcc
      481 gcgggtcgtc ggcgcgacga tgaacacgag gtcgaggccg aactcgtcgc aggcctcgcg
      541 gagcgggccg gcctcctcgg ccggcaggtc ggggacgacg aagccctcga taccgacttc
      601 cgccgccttc tcgacgaacg gccgcggtcc gggttcatcg ccgtagcgat agatgaggtt
      661 gtagtaggtc atacagacca gcggcaccga cacgtcgagg tcctcgacga actcgaaaaa
      721 gcgcgtcggc gtcatgccgc cttcgagcga ccgaacaacc gcgttctgga tggtcggccc
      781 ctcggcgatg ggctccgaga acggaagtcc gagttcgatg acgtccgcgc cgccgcgttc
      841 gagcgcctcg acgtattcga gcgaggactc gtagtcgggg tcgccggcgg caagataggg
      901 gacgaacgcc gggccgtcgg agaaggcgtc ttcgagcgac atggctgcag agtgcggctt
      961 ccgaacgcat agtagttgct gttcgggcca gcgtactgcc acgaaagccc gggtgtgacc
     1021 gccatcacga cgctgcccgc ggcgtcaacg aggggtttta tccacgggat ctaaatcaaa
     1081 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     1141 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     1201 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     1261 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     1321 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     1381 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     1441 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     1501 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     1561 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     1621 attgggtacc gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcga
     1681 tatcggtacc aagcttacgc cgaaaaatgc gatggtccag aggtgctcat ctagattacc
     1741 agacttgcga cgggagcggc gagtatcgcg cgaccgcgtg ctcgtagagc ttgactttgt
     1801 tgtagtccga gtcgttgccg aggatctcga ttcggtggtc gacgaagtgc gcgtccggga
     1861 gctggacgac cttcttcgcc ttgtacgtcg tcttgaagtc gcagaggtag tggccgccgc
     1921 cttcgaggag cagcgccatg ttgatgttgc cgacgagcag gccgtcgcgg acgtggagct
     1981 tctcggtcga cggctcccac ttgagcgtct ttttctgcat gaccgggccg ttcggcggga
     2041 agttcgtgcc cttgaagcgg acgttctgga agaagcagtc gccttcgagc gagatgtccg
     2101 agcggatcgt gcagatgccc ttgtcctcga acgtcatcgt gcgctcccac gagtagccct
     2161 cggggaacga ctgcttgaag tagtccggga tgtcctcggg gtacttcgtg aacacgcggt
     2221 tgccgtagtg gaccgccgtc gtgaggatgt cgtacgagaa cgggagcggg gcgccttctt
     2281 tgaccgtgag gttcgccgtc tgcgtgcctt cgtacggctt gcccttgcct tcgccttcga
     2341 tgacgaacgc gtggccgttg acgttgcctt ccatgtggac cttgacgcgc atgtcctctt
     2401 tgatgaggtt gatgcccggc gtgttcatat gcagctctct agccgggggg cacttataag
     2461 cgtttcgatt ctcggatccg agctcgcggc cgccaccgcg gtggagctcc agcttttgtt
     2521 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     2581 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     2641 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     2701 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     2761 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     2821 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     2881 caggggataa cgcaggaaag aacatggggg gttgttgtcc gaccgcgatt cataggttca
     2941 gagttcgtcg accctactct aatcatgaac gtactgtctc gagagatacc acgagattac
     3001 gagatacccg aatacaacgc atatcacgaa catattacca aaggttagtt caatcacgaa
     3061 cctgcctccg tgaggggttg ctgtgcaatc tccggcccaa tagacagcgt ctgaaccgtc
     3121 tcgtctcgtt cttcggttag actcgacagt aagggacgta cccagtctac ccagtcgtac
     3181 atcgtatgga gacggtcgat gtccggtagc ccgttaggtc cgcattgtac ccatatctcg
     3241 attacctcct cggtcaactc atcaagcgct tctagcgtct caaagaggac gtctcgagtg
     3301 aacgactcgg tcagcatgaa cgatggaaga atccgcttgt ggcgttcttg ctcgtctgtg
     3361 aacgaaaggt tcagtcggca ctgaccctcg gtctcgtcga ctaaccccat tgctacaagt
     3421 gtcggtaagt ggttccgaac cgactgcgtc gagattcccg ctttctcggc taactctgtc
     3481 ttgctaaggc tcagattcgt cgcgagcaac gtactaatca tcgcccgaac cgtgggggtt
     3541 gcgtcctcta agagacgctt cgacgggaga ctcgcgagtg aaagccgtac ttcgctactc
     3601 cgaatctctc tcggtgtcgc ttcttgagac aaccctcgcc cgattgcgtg ggctacgtcg
     3661 tacggtgtcc gtgcaaaccc agagaatagc cgtatcgcgt ctctcgtcgc gtcgagacct
     3721 ttcgcccgac acattgcccg tgttactcgt gcaaccgtac tcggaccatg ctcgacgacc
     3781 acagggactt tgagcgcaat ctccggcgcg tcctcgtgaa tctccccagg gttcgacagt
     3841 cggtcttcta ttacgccggc gacgtggtcc gcccgattcc cgaagtcacc aaccaccgag
     3901 atcgacgcaa tcaggttcgc atacggattg ctcgcgtcga cgacagggtc gaacgactga
     3961 cgtcgcttct cgtctcggtc ttcaaacaac agtcgccgga ctacgtggtt cccgtacgtc
     4021 gccgcaatcg gcgcgttctt cgcaagcgtc gttgctaaca actcggcatt gtcctccccg
     4081 tatcgctccg ggtgtcggtt gtagtcgggt aagcgagcct cccaaacgac gtcaatatcg
     4141 agtaaatcga ggacgtgaaa cgcggttccc gcgagaccca tcgccgtttt cgtgattcga
     4201 ctcaggtact cggacgtctc acactcgtag tcctcatgtg caagtgaccg cgtcatgtcg
     4261 agtagctcgt ctctcgcgtc ctcgaggacg tcgacgtact cacccgccga gtcgacagag
     4321 tcagccaacc acccgaggtt tcgagaccca cgaacgtacg tcccatcttc gattagtcca
     4381 tcaagttccg cgagacggtc ctcggtaagg acctggtcga acgtcttagg atcgagtaac
     4441 gctcgtgcaa tcatcaccac atgagacatg gcagagtcgt actctgcaga gacgacgagc
     4501 gtattctcgt cctcaaggta gccccacccg gcttctcccc ggtcagattt gggttcgaca
     4561 gggtagtttg tcacactaac gcctttcttc ggcggtgtct tcgccgctga gaccgtctct
     4621 gcgtacgaaa gatagtccac accgtgatac cgaggtaagc gaacccgtcc gtccggcgtc
     4681 gtcgacgcct caccttcggc ggtcggtcgg tggtcgtcct ccctccctcc ccgagggcgg
     4741 tccgtgatta cacgggtatt gtggtttggt tttggggtgt tcgtaacgta gtcctgaagg
     4801 gccttttgtc gctctttctc tttggctaat tcttcgaggt acaacgctcc tttgtcgaga
     4861 atctcgattc gtaggtttcc gcgagcgccg tacttctcac aaagaccata ctcgattagc
     4921 ttctcgttca atcgccgcga gaccgtggtc ctatcgtact gactgaacgc cgattgtagg
     4981 gcgctgtagg ccatgtgtcc gctttcagtc tcacctaccg tccgaagtat ctcgacagca
     5041 agtccgtccg tcccaaccac gtcgacgagc gtcgtaatgg agtaatcatt acgggtgtcg
     5101 ttgcacggat tcgtaacact cgatgggaga atatcgccgt agtagttgtg caagtacact
     5161 cggtcgatct ctgacccacc aaggaccacg tcgacgctct cagacagcgt tagaacgtac
     5221 tcgataatct cgtcggcctg gtcgcgccgc gtctctctga aagccgcatt gagaccgatt
     5281 gctatcgtct gttgagggtt gctatagccc aatagacgag tgaacgcgag agcgtcctct
     5341 aaggcgctct gtggctgtcc catctcatca tgtcccaacc gtcgcgcata ccgaatcaag
     5401 tcccacagag cccgtctctc gccggcgaca gctcgtcgtg caagtttcac gactgaccca
     5461 tcgacgtcaa acccacaact cgacagtcgg tgttcaaact cgagtatgtc gacaccgtcc
     5521 gccgctgttt gtagtgcctc gagaagcgca aattgactct ctgtctcgtc gaagattact
     5581 cgatgaaccc gaagggcggt ccagtctgta agcgagacgt ccgcctcgag gtcagtcgca
     5641 aagcgaaggc ttcgcttctc agtcggttcg tgtgccgtcg tatcgtccgt atcgtacatt
     5701 tagtgtcgct tttgtgcgaa cactaacgcc gggtgaaaat ccgataatcc cataagcccc
     5761 aaccacattg gttgagttag gacaacgggc cacccgcccg ccgtgtccgc gtaagcatcc
     5821 ttcgacgccg ataggagcga agtcctggca gactcgtgtg ctcccggcgt cgtttgattc
     5881 aactagttcg ctagtcacga ctatagttta ttaatccacc ttctcagaga gatatgcgtc
     5941 aagcaccacc gcaattgtct catcgtatga ccgtcggcct tcccgcgcct tgagaacccg
     6001 gaggtcgcgc cgcacatcct tcgacaccgg aatccgcgtg tccgtggcag acatatatcc
     6061 cgctttaggg cacaacgtat aaagcattac ggacatgaag atgtgattgg taaagcatga
     6121 gtcttgacga aagggcaaga gggattttgt caccggccga ccgccggtat ctccaaaaac
     6181 cgaaggagta tagcaaacag gcaggatacg aacgccggcg agcgatagtc gagcggctcc
     6241 atgaagccct acatgatatg ccgctcctgg tttcggaact cgacgaggac cttagaacgg
     6301 aagccttcga ggacggcgat ttagacggga aagagcatac gctaaacgtc ctgtcgtggt
     6361 cgttcgctct cctatatctc gggatcaccg acaccgtcga accgtccgat ttggcaaagg
     6421 acgctttcga ggggattgcc gcgagcggtg ttagacaggc atacctccag cgcgggtatt
     6481 cggtcgagga ggtcgaggtc gaggttaatg taaaacgggg cgagccgctc gacgagttac
     6541 gggagcgcga gccggacgaa ctcagttacc ccgaggtgaa ccaactactc gaatcgggcg
     6601 aactctccca cctaaccggc gaggaacaaa tggctcgaat caaagaagct catgtgagca
     6661 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6721 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     6781 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     6841 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     6901 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     6961 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7021 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7081 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7141 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7201 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7261 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7321 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7381 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7441 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7501 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7561 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7621 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7681 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7741 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7801 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7861 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     7921 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7981 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8041 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8101 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8161 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8221 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8281 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8341 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8401 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
