LOCUS       sequence-325187-        8476 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of H. volcanii codon-optimized PAmCherry1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8476)
  AUTHORS   Turkowyd B, Schreiber S, Wortz J, Segal ES, Mevarech M, Duggin IG, 
            Marchfelder A, Endesfelder U
  TITLE     Establishing Live-Cell Single-Molecule Localization Microscopy 
            Imaging and Single-Particle Tracking in the Archaeon Haloferax 
            volcanii.
  JOURNAL   Front Microbiol. 2020 Nov 20;11:583010. doi: 
            10.3389/fmicb.2020.583010. eCollection 2020.
  PUBMED    33329447
REFERENCE   2  (bases 1 to 8476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2020-11-20"; volume: "11:583010. doi"; pages: " 
            10.3389/fmicb.2020.583010. eCollection 2020"
COMMENT     Sequence Label: pTA231-p.Syn-PAmCherry1Hfx
FEATURES             Location/Qualifiers
     source          1..8476
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5650..5667
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5784..5805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5820..5825,5826..5843,5844..5850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5858..5874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5863..5885
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5882..5898
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5882..5898
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5917..5937
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5919..5937
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6819..6835)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6820..6836)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(6860..6879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6861..6879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6889..6906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6889..6905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6898..6920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7047..7405
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7134..7153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7344..7365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgag cttctttgat tcgagccatt tgttcctcgc cggttaggtg ggagagttcg
     1861 cccgattcga gtagttggtt cacctcgggg taactgagtt cgtccggctc gcgctcccgt
     1921 aactcgtcga gcggctcgcc ccgttttaca ttaacctcga cctcgacctc ctcgaccgaa
     1981 tacccgcgct ggaggtatgc ctgtctaaca ccgctcgcgg caatcccctc gaaagcgtcc
     2041 tttgccaaat cggacggttc gacggtgtcg gtgatcccga gatataggag agcgaacgac
     2101 cacgacagga cgtttagcgt atgctctttc ccgtctaaat cgccgtcctc gaaggcttcc
     2161 gttctaaggt cctcgtcgag ttccgaaacc aggagcggca tatcatgtag ggcttcatgg
     2221 agccgctcga ctatcgctcg ccggcgttcg tatcctgcct gtttgctata ctccttcggt
     2281 ttttggagat accggcggtc ggccggtgac aaaatccctc ttgccctttc gtcaagactc
     2341 atgctttacc aatcacatct tcatgtccgt aatgctttat acgttgtgcc ctaaagcggg
     2401 atatatgtct gccacggaca cgcggattcc ggtgtcgaag gatgtgcggc gcgacctccg
     2461 ggttctcaag gcgcgggaag gccgacggtc atacgatgag acaattgcgg tggtgcttga
     2521 cgcatatctc tctgagaagg tggattaata aactatagtc gtgactagcg aactagttga
     2581 atcaaacgac gccgggagca cacgagtctg ccaggacttc gctcctatcg gcgtcgaagg
     2641 atgcttacgc ggacacggcg ggcgggtggc ccgttgtcct aactcaacca atgtggttgg
     2701 ggcttatggg attatcggat tttcacccgg cgttagtgtt cgcacaaaag cgacactaaa
     2761 tgtacgatac ggacgatacg acggcacacg aaccgactga gaagcgaagc cttcgctttg
     2821 cgactgacct cgaggcggac gtctcgctta cagactggac cgcccttcgg gttcatcgag
     2881 taatcttcga cgagacagag agtcaatttg cgcttctcga ggcactacaa acagcggcgg
     2941 acggtgtcga catactcgag tttgaacacc gactgtcgag ttgtgggttt gacgtcgatg
     3001 ggtcagtcgt gaaacttgca cgacgagctg tcgccggcga gagacgggct ctgtgggact
     3061 tgattcggta tgcgcgacgg ttgggacatg atgagatggg acagccacag agcgccttag
     3121 aggacgctct cgcgttcact cgtctattgg gctatagcaa ccctcaacag acgatagcaa
     3181 tcggtctcaa tgcggctttc agagagacgc ggcgcgacca ggccgacgag attatcgagt
     3241 acgttctaac gctgtctgag agcgtcgacg tggtccttgg tgggtcagag atcgaccgag
     3301 tgtacttgca caactactac ggcgatattc tcccatcgag tgttacgaat ccgtgcaacg
     3361 acacccgtaa tgattactcc attacgacgc tcgtcgacgt ggttgggacg gacggacttg
     3421 ctgtcgagat acttcggacg gtaggtgaga ctgaaagcgg acacatggcc tacagcgccc
     3481 tacaatcggc gttcagtcag tacgatagga ccacggtctc gcggcgattg aacgagaagc
     3541 taatcgagta tggtctttgt gagaagtacg gcgctcgcgg aaacctacga atcgagattc
     3601 tcgacaaagg agcgttgtac ctcgaagaat tagccaaaga gaaagagcga caaaaggccc
     3661 ttcaggacta cgttacgaac accccaaaac caaaccacaa tacccgtgta atcacggacc
     3721 gccctcgggg agggagggag gacgaccacc gaccgaccgc cgaaggtgag gcgtcgacga
     3781 cgccggacgg acgggttcgc ttacctcggt atcacggtgt ggactatctt tcgtacgcag
     3841 agacggtctc agcggcgaag acaccgccga agaaaggcgt tagtgtgaca aactaccctg
     3901 tcgaacccaa atctgaccgg ggagaagccg ggtggggcta ccttgaggac gagaatacgc
     3961 tcgtcgtctc tgcagagtac gactctgcca tgtctcatgt ggtgatgatt gcacgagcgt
     4021 tactcgatcc taagacgttc gaccaggtcc ttaccgagga ccgtctcgcg gaacttgatg
     4081 gactaatcga agatgggacg tacgttcgtg ggtctcgaaa cctcgggtgg ttggctgact
     4141 ctgtcgactc ggcgggtgag tacgtcgacg tcctcgagga cgcgagagac gagctactcg
     4201 acatgacgcg gtcacttgca catgaggact acgagtgtga gacgtccgag tacctgagtc
     4261 gaatcacgaa aacggcgatg ggtctcgcgg gaaccgcgtt tcacgtcctc gatttactcg
     4321 atattgacgt cgtttgggag gctcgcttac ccgactacaa ccgacacccg gagcgatacg
     4381 gggaggacaa tgccgagttg ttagcaacga cgcttgcgaa gaacgcgccg attgcggcga
     4441 cgtacgggaa ccacgtagtc cggcgactgt tgtttgaaga ccgagacgag aagcgacgtc
     4501 agtcgttcga ccctgtcgtc gacgcgagca atccgtatgc gaacctgatt gcgtcgatct
     4561 cggtggttgg tgacttcggg aatcgggcgg accacgtcgc cggcgtaata gaagaccgac
     4621 tgtcgaaccc tggggagatt cacgaggacg cgccggagat tgcgctcaaa gtccctgtgg
     4681 tcgtcgagca tggtccgagt acggttgcac gagtaacacg ggcaatgtgt cgggcgaaag
     4741 gtctcgacgc gacgagagac gcgatacggc tattctctgg gtttgcacgg acaccgtacg
     4801 acgtagccca cgcaatcggg cgagggttgt ctcaagaagc gacaccgaga gagattcgga
     4861 gtagcgaagt acggctttca ctcgcgagtc tcccgtcgaa gcgtctctta gaggacgcaa
     4921 cccccacggt tcgggcgatg attagtacgt tgctcgcgac gaatctgagc cttagcaaga
     4981 cagagttagc cgagaaagcg ggaatctcga cgcagtcggt tcggaaccac ttaccgacac
     5041 ttgtagcaat ggggttagtc gacgagaccg agggtcagtg ccgactgaac ctttcgttca
     5101 cagacgagca agaacgccac aagcggattc ttccatcgtt catgctgacc gagtcgttca
     5161 ctcgagacgt cctctttgag acgctagaag cgcttgatga gttgaccgag gaggtaatcg
     5221 agatatgggt acaatgcgga cctaacgggc taccggacat cgaccgtctc catacgatgt
     5281 acgactgggt agactgggta cgtcccttac tgtcgagtct aaccgaagaa cgagacgaga
     5341 cggttcagac gctgtctatt gggccggaga ttgcacagca acccctcacg gaggcaggtt
     5401 cgtgattgaa ctaacctttg gtaatatgtt cgtgatatgc gttgtattcg ggtatctcgt
     5461 aatctcgtgg tatctctcga gacagtacgt tcatgattag agtagggtcg acgaactctg
     5521 aacctatgaa tcgcggtcgg acaacaaccc cccatgttct ttcctgcgtt atcccctgat
     5581 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5641 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5701 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5761 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5821 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5881 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     5941 caaaagctgg agctccaccg cggtggcggc cgcgagctcg gatccgagaa tcgaaacgct
     6001 tataagtgcc ccccggctag agagctgcat atggtcagca agggcgaaga ggacaacatg
     6061 gcgatcatca aagaattcat gcgcttcaag gtccacatgg aaggctcggt caacggccac
     6121 gtgttcgaga tcgagggcga gggcgaaggc cgaccgtacg aaggcacgca gacggcgaag
     6181 ctcaaagtca cgaagggtgg cccgctcccg ttcacgtggg acatcctctc gccgcagttc
     6241 atgtacggct cgaacgcgta cgtcaagcac ccggcggaca tcccggacta cttcaagctc
     6301 tcgttccccg agggcttcaa gtgggagcgc gtcatgaagt tcgaggacgg cggcgtcgtc
     6361 acggtcacgc aggactcgtc gctgcaggac ggcgagttca tctacaaagt caagctccgc
     6421 ggcacgaact tcccgtcgga cggcccggtc atgcagaaaa agacgatggg ctgggaggcg
     6481 ctctcggagc gcatgtaccc cgaggacggg gcgctcaaag gcgaagtcaa gccgcgcgtc
     6541 aagctcaaag acggcggcca ctacgacgcg gaagtcaaga cgacgtacaa ggcgaagaag
     6601 ccggtccagc tccccggtgc ctacaacgtc aaccgcaagc tcgacatcac gtcgcacaac
     6661 gaggactaca cgatcgtcga gcagtacgag cgcgcggaag gccgacactc gacgggcggc
     6721 atggacgaac tctacaaatg atctagatga gcacctctgg accatcgcat ttttcggcgt
     6781 aagcttggta ccgatatcga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     6841 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6901 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6961 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     7021 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     7081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7381 tatctcggtc tattcttttg atttagatcc cgtggataaa acccctcgtt gacgccgcgg
     7441 gcagcgtcgt gatggcggtc acacccgggc tttcgtggca gtacgctggc ccgaacagca
     7501 actactatgc gttcggaagc cgcactctgc agccatgtcg ctcgaagacg ccttctccga
     7561 cggcccggcg ttcgtcccct atcttgccgc cggcgacccc gactacgagt cctcgctcga
     7621 atacgtcgag gcgctcgaac gcggcggcgc ggacgtcatc gaactcggac ttccgttctc
     7681 ggagcccatc gccgaggggc cgaccatcca gaacgcggtt gttcggtcgc tcgaaggcgg
     7741 catgacgccg acgcgctttt tcgagttcgt cgaggacctc gacgtgtcgg tgccgctggt
     7801 ctgtatgacc tactacaacc tcatctatcg ctacggcgat gaacccggac cgcggccgtt
     7861 cgtcgagaag gcggcggaag tcggtatcga gggcttcgtc gtccccgacc tgccggccga
     7921 ggaggccggc ccgctccgcg aggcctgcga cgagttcggc ctcgacctcg tgttcatcgt
     7981 cgcgccgacg acccgcggcg agcgcctcga ccgaatcatg gaacaggtct cgggctacgt
     8041 ctacgtgcag gcgcgcctcg gcacgacggg cgcgcagtcg agcgtctccg accagaccga
     8101 ctcgtcgctc gaacgactca ccgactacga cgtgcccaag gcggtcggct tcggaatcag
     8161 cgacggcgac cacgccgagc gcatcgtcgc cagcggtgcc gacggcatca tcgtcggcag
     8221 cgcgctcgtc gacatcgtcg ccgagggcca cgagaacggc gacgacgccg aaacggtcgc
     8281 ggaccgactc gaaacgctcg cccgcgaact cgaagacggt gcggtagcgg gcgcgtcgca
     8341 acgcccaccg catccggaac gcacataact gctctagtaa gggattttgc cgatttcggc
     8401 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     8461 aacgcttaca atttag
//
