LOCUS       sequence-325189-       13789 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express CARPID BASU-dCasRx fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Yi W, Li J, Zhu X, Wang X, Fan L, Sun W, Liao L, Zhang J, Li X, Ye 
            J, Chen F, Taipale J, Chan KM, Zhang L, Yan J
  TITLE     CRISPR-assisted detection of RNA-protein interactions in living 
            cells.
  JOURNAL   Nat Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0.
  PUBMED    32572232
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2020 Jun 22. pii: 10.1038/s41592-020-0866-0. doi: 
            10.1038/s41592-020-0866-0."
COMMENT     Sequence Label: CARPID BASU-dCasRx
FEATURES             Location/Qualifiers
     source          1..13789
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2445..3623
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2676..3614
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3571..3591
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4440..4460
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4470..7367
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             7377..7397
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7407..7433
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7449..7502
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7503..7505,7506..8219)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(7503..7505,7506..8216)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7545..7566)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7806..7825)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8153..8174
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    8244..8832
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8297..8317)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8715..8726)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8835..8851)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8836..8852)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9357..9537
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9563..9580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9569..9793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9839..10267
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9926..9945)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10136..10157
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10276..10296)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10281..10610
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10461..10596
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10523..10542
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10658..10705
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10724..11098
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11228..11349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11265..11284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11319..11338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11398..11414)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11398..11414)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11411..11433)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11422..11438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11446..11452,11453..11470,11471..11476))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11491..11512
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11629..11646)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11800..12388)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11880..11899)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12559..13350,13351..13419))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13182..13201
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13420..13524)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13599..13618)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa ttaatgcaaa gatggataaa gttttaaaca gagaggaatc
     2401 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2461 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2521 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2581 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2641 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2701 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2761 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2821 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2881 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2941 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     3001 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     3061 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     3121 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     3181 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     3241 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3301 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3361 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3421 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3481 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3541 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3601 ttcttccatt tcaggtgtcg tgacgtacgg ccaccatgag cggcaagttg tctgaatcag
     3661 agattaggtt tggactgaaa acggaggtga tgggccaaca tcttatctat cacgatgttt
     3721 tgtccagcac acaaaagaca gcccatgaat tggcaaataa caatgccccc gagggtaccc
     3781 tcgtggttgc agataaacag acagccggta ggggcgggat gagccgagtg tggcattctc
     3841 aagaaggaaa cggagtatgg atgagtttga tattgcgccc ggacattccg ctccaaaaga
     3901 cgccccaact taccttgctg gcagctgtag cggtggttca gggaatagaa gaggccgcgg
     3961 gcatacagac tgatataaag tggccgaacg acatactcat taacgggaaa aaaacagtag
     4021 ggatacttac cgaaatgcaa gctgaggagg atcgagtgcg ctccgtgatt attggtattg
     4081 gcattaatgt aaatcagcaa cccaatgatt tccccgatga gctcaaggac attgcgacga
     4141 gtctgtccca ggcagctggg gaaaagatcg acagggcagg tgtaatccag catatcttgc
     4201 tttgtttcga aaagcgatac cgagactaca tgacccacgg tttcacgccc atcaagctct
     4261 tgtgggagtc atacgccctc ggtattggca cgaacatgag ggcgagaact ctcaacggga
     4321 cattttacgg taaggcgttg ggtattgacg acgaaggggt acttttgttg gaaactaatg
     4381 aaggcattaa gaagatatac agcgcggaca tcagccttag gcgtacggcc accatgagcc
     4441 ccaagaagaa gagaaaggtg gaggccagca tcgaaaaaaa aaagtccttc gccaagggca
     4501 tgggcgtgaa gtccacactc gtgtccggct ccaaagtgta catgacaacc ttcgccgaag
     4561 gcagcgacgc caggctggaa aagatcgtgg agggcgacag catcaggagc gtgaatgagg
     4621 gcgaggcctt cagcgctgaa atggccgata aaaacgccgg ctataagatc ggcaacgcca
     4681 aattcagcca tcctaagggc tacgccgtgg tggctaacaa ccctctgtat acaggacccg
     4741 tccagcagga tatgctcggc ctgaaggaaa ctctggaaaa gaggtacttc ggcgagagcg
     4801 ctgatggcaa tgacaatatt tgtatccagg tgatccataa catcctggac attgaaaaaa
     4861 tcctcgccga atacattacc aacgccgcct acgccgtcaa caatatctcc ggcctggata
     4921 aggacattat tggattcggc aagttctcca cagtgtatac ctacgacgaa ttcaaagacc
     4981 ccgagcacca tagggccgct ttcaacaata acgataagct catcaacgcc atcaaggccc
     5041 agtatgacga gttcgacaac ttcctcgata accccagact cggctatttc ggccaggcct
     5101 ttttcagcaa ggagggcaga aattacatca tcaattacgg caacgaatgc tatgacattc
     5161 tggccctcct gagcggactg gcgcactggg tggtcgctaa caacgaagaa gagtccagga
     5221 tctccaggac ctggctctac aacctcgata agaacctcga caacgaatac atctccaccc
     5281 tcaactacct ctacgacagg atcaccaatg agctgaccaa ctccttctcc aagaactccg
     5341 ccgccaacgt gaactatatt gccgaaactc tgggaatcaa ccctgccgaa ttcgccgaac
     5401 aatatttcag attcagcatt atgaaagagc agaaaaacct cggattcaat atcaccaagc
     5461 tcagggaagt gatgctggac aggaaggata tgtccgagat caggaaaaat cataaggtgt
     5521 tcgactccat caggaccaag gtctacacca tgatggactt tgtgatttat aggtattaca
     5581 tcgaagagga tgccaaggtg gctgccgcca ataagtccct ccccgataat gagaagtccc
     5641 tgagcgagaa ggatatcttt gtgattaacc tgaggggctc cttcaacgac gaccagaagg
     5701 atgccctcta ctacgatgaa gctaatagaa tttggagaaa gctcgaaaat atcatgcaca
     5761 acatcaagga atttagggga aacaagacaa gagagtataa gaagaaggac gcccctagac
     5821 tgcccagaat cctgcccgct ggccgtgatg tttccgcctt cagcaaactc atgtatgccc
     5881 tgaccatgtt cctggatggc aaggagatca acgacctcct gaccaccctg attaataaat
     5941 tcgataacat ccagagcttc ctgaaggtga tgcctctcat cggagtcaac gctaagttcg
     6001 tggaggaata cgcctttttc aaagactccg ccaagatcgc cgatgagctg aggctgatca
     6061 agtccttcgc tagaatggga gaacctattg ccgatgccag gagggccatg tatatcgacg
     6121 ccatccgtat tttaggaacc aacctgtcct atgatgagct caaggccctc gccgacacct
     6181 tttccctgga cgagaacgga aacaagctca agaaaggcaa gcacggcatg agaaatttca
     6241 ttattaataa cgtgatcagc aataaaaggt tccactacct gatcagatac ggtgatcctg
     6301 cccacctcca tgagatcgcc aaaaacgagg ccgtggtgaa gttcgtgctc ggcaggatcg
     6361 ctgacatcca gaaaaaacag ggccagaacg gcaagaacca gatcgacagg tactacgaaa
     6421 cttgtatcgg aaaggataag ggcaagagcg tgagcgaaaa ggtggacgct ctcacaaaga
     6481 tcatcaccgg aatgaactac gaccaattcg acaagaaaag gagcgtcatt gaggacaccg
     6541 gcagggaaaa cgccgagagg gagaagttta aaaagatcat cagcctgtac ctcaccgtga
     6601 tctaccacat cctcaagaat attgtcaata tcaacgccag gtacgtcatc ggattccatt
     6661 gcgtcgagcg tgatgctcaa ctgtacaagg agaaaggcta cgacatcaat ctcaagaaac
     6721 tggaagagaa gggattcagc tccgtcacca agctctgcgc tggcattgat gaaactgccc
     6781 ccgataagag aaaggacgtg gaaaaggaga tggctgaaag agccaaggag agcattgaca
     6841 gcctcgagag cgccaacccc aagctgtatg ccaattacat caaatacagc gacgagaaga
     6901 aagccgagga gttcaccagg cagattaaca gggagaaggc caaaaccgcc ctgaacgcct
     6961 acctgaggaa caccaagtgg aatgtgatca tcagggagga cctcctgaga attgacaaca
     7021 agacatgtac cctgttcgca aacaaggccg tcgccctgga agtggccagg tatgtccacg
     7081 cctatatcaa cgacattgcc gaggtcaatt cctacttcca actgtaccat tacatcatgc
     7141 agagaattat catgaatgag aggtacgaga aaagcagcgg aaaggtgtcc gagtacttcg
     7201 acgctgtgaa tgacgagaag aagtacaacg ataggctcct gaaactgctg tgtgtgcctt
     7261 tcggctactg tatccccagg tttaagaacc tgagcatcga ggccctgttc gataggaacg
     7321 aggccgccaa gttcgacaag gagaaaaaga aggtgtccgg caattccgga tccggaccta
     7381 agaaaaagag gaaggtggcg gccgcttacc catacgatgt tccagattac gctgctagcg
     7441 gcagtggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag aatcctggcc
     7501 cagtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     7561 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     7621 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     7681 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     7741 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     7801 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     7861 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     7921 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     7981 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     8041 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     8101 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     8161 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag
     8221 aattcgatat caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga
     8281 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     8341 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     8401 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     8461 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     8521 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     8581 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
     8641 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8701 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8761 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     8821 ccgcctcccc gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa
     8881 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     8941 aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg
     9001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     9061 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc
     9121 agaactacac accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc
     9181 tagtaccagt tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt
     9241 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt
     9301 ttgacagccg cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt
     9361 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9421 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9481 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg
     9541 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     9601 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     9661 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     9721 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     9781 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     9841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     9901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     9961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10021 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
    10081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10141 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
    10201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
    10261 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
    10321 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
    10381 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
    10441 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
    10501 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
    10561 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
    10621 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata
    10681 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag
    10741 tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg
    10801 gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt
    10861 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt
    10921 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt
    10981 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt
    11041 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca
    11101 cgtgctacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
    11161 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
    11221 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    11281 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    11341 tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc
    11401 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
    11461 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
    11521 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
    11581 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
    11641 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
    11701 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
    11761 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
    11821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
    11881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
    11941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
    12001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
    12061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
    12121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
    12181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
    12241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
    12301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
    12361 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
    12421 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
    12481 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
    12541 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
    12601 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
    12661 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
    12721 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
    12781 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
    12841 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
    12901 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
    12961 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
    13021 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
    13081 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
    13141 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
    13201 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
    13261 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
    13321 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
    13381 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
    13441 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
    13501 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg
    13561 atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca
    13621 tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc
    13681 aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg
    13741 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtt
//
