LOCUS       sequence-32523-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Peti W, Page R
  TITLE     Strategies to maximize heterologous protein expression in 
            Escherichia coli with minimal cost.
  JOURNAL   Protein Expr Purif. 2007 Jan . 51(1):1-10.
  PUBMED    16904906
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2007 Jan . 51(1):1-10."
COMMENT     Sequence Label: RP1B
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             81..101
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             154..171
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(224..242)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      238..285
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      322..777
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(409..428)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     619..640
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcatca tcatcatcac gaaaatcttt attttcaagg tcatatggga tccgaattcg
      121 agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca ctgagatccg
      181 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
      241 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
      301 atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
      361 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
      421 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
      481 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
      541 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
      601 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
      661 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
      721 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
      781 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
      841 aaatatgtat ccgctcatga attaattctt agaa
//
