LOCUS       sequence-32525-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pFRT_DESTFLAGHA
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      136..145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             145..168
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             169..195
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    211..335
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(360..365,366..383,384..390)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(510..529)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gagctcggat cgatccatcg atagtgtggt
      121 ggaattctga tcgccgccgc catggactac aaggacgacg atgacaagta cccttatgac
      181 gtgcccgatt acgctttgaa ccagatatca acaagtttgt acaaaaaagc tgaacgagaa
      241 acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa acagactaca
      301 taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc accccaggct
      361 ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat ccgtcgagat
      421 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      481 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      541 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      601 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      661 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      721 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      781 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      841 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      901 tcaccagttt tgattta
//
