LOCUS       sequence-32526-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pFRT_TO_DESTFLAGHA
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..19
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     50..74
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             174..197
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             198..224
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    240..364
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(389..394,395..412,413..419)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(539..558)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga gctcgtttag
       61 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagacacc
      121 gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc atggactaca
      181 aggacgacga tgacaagtac ccttatgacg tgcccgatta cgctttgaac cagatatcaa
      241 caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa
      301 attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac
      361 tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
      421 gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa
      481 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
      541 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
      601 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
      661 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
      721 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
      781 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
      841 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtt
//
