LOCUS       sequence-325284-       12843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative plasmid for cassette TDH3p:his6-mKate2-LipPks1-IDP1t.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Romero-Suarez D, Wulff T, Rong Y, Jakociu Nas T, Yuzawa S, Keasling 
            JD, Jensen MK
  TITLE     A Reporter System for Cytosolic Protein Aggregates in Yeast.
  JOURNAL   ACS Synth Biol. 2021 Feb 12. doi: 10.1021/acssynbio.0c00476.
  PUBMED    33577304
REFERENCE   2  (bases 1 to 12843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2021 Feb 12. doi: 10.1021/acssynbio.0c00476."
COMMENT     Sequence Label: pYR14 (XII-5:TDH3p:his6-mKate2-LipPks1-IDP1t)
FEATURES             Location/Qualifiers
     source          1..12843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        486..1152
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     1022..1045
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             1171..1188
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(10024..10057)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(10527..10544)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10527..10543)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10642..10659)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10813..11401)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(11525..12316,12317..12385))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12148..12167
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12386..12477)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12519..12538
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12544..12563
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    12770..12803
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 caatctggcg gcttgagttc tcaacatgtt ttatttttta cttatattgc tggtagggta
       61 aaaaaatata actcctagga ataggttgtc tatatgtttt tgtcttgctt ctataattgt
      121 aacaaacaag gaaagggaaa atactgggtg taaaagccat tgagtcaagt taggtcatcc
      181 cttttataca aaatttttca attttttttc caagattctt gtacgattaa ttattttttt
      241 tttgcgtcct acagcgtgat gaaaatttcg cctgctgcaa gatgagcggg aacgggcgaa
      301 atgtgcacgc gcacaactta cgaaacgcgg atgagtcact gacagccacc gcagaggttc
      361 tgactcctac tgagctctat tggaggtggc agaaccggta ccggaggaga ccgctataac
      421 cggtttgaat ttattgtcac agtgtcacat cagcataaaa aacacgcttt ttcagttcga
      481 gtttatcatt atcaatactg ccatttcaaa gaatacgtaa ataattaata gtagtgattt
      541 tcctaacttt atttagtcaa aaaattagcc ttttaattct gctgtaaccc gtacatgccc
      601 aaaatagggg gcgggttaca cagaatatat aacatcgtag gtgtctgggt gaacagttta
      661 ttcctggcat ccactaaata taatggagcc cgctttttaa gctggcatcc agaaaaaaaa
      721 agaatcccag caccaaaata ttgttttctt caccaaccat cagttcatag gtccattctc
      781 ttagcgcaac tacagagaac aggggcacaa acaggcaaaa aacgggcaca acctcaatgg
      841 agtgatgcaa cctgcctgga gtaaatgatg acacaaggca attgacccac gcatgtatct
      901 atctcatttt cttacacctt ctattacctt ctgctctctc tgatttggaa aaagctgaaa
      961 aaaaaggttg aaaccagttc cctgaaatta ttcccctact tgactaataa gtatataaag
     1021 acggtaggta ttgattgtaa ttctgtaaat ctatttctta aacttcttaa attctacttt
     1081 tatagttagt ctttttttta gttttaaaac accaagaact tagtttcgaa taaacacaca
     1141 taaacaaaca aaaaaacaat gggtagcagc caccaccacc accaccacgg cggtggcggt
     1201 tctatggttt ctgaactcat caaggaaaac atgcacatga aactttacat ggaaggtact
     1261 gtgaacaatc atcattttaa gtgtacatcc gagggtgaag gcaaacctta cgaaggaact
     1321 caaactatga gaattaaagc tgtagaaggt ggaccattac cttttgcatt tgatatcttg
     1381 gcaacatcat tcatgtatgg gagcaagaca ttcataaacc atactcaagg tataccagac
     1441 tttttcaaac agagttttcc agagggtttt acatgggaaa gagtaacaac gtacgaggat
     1501 ggaggtgtat tgacagccac tcaagacaca tcacttcaag atgggtgttt aatctacaat
     1561 gtcaagatta gaggcgtcaa tttcccttct aatggtccag ttatgcagaa aaagacatta
     1621 ggctgggaag cgtcaaccga aaccctgtac cctgctgatg gtggcctaga aggcagagct
     1681 gacatggccc ttaaactggt tggtggaggg catctaatct gcaatttgaa aaccacttat
     1741 cgttctaaaa agccagccaa aaacctaaag atgccaggtg tttactacgt cgaccgaaga
     1801 ttagaaagga ttaaagaggc tgataaagag acttatgttg aacaacacga agtggcagtg
     1861 gctagatact gtgatttgcc atctaagttg ggacacagag acggtggatc cgggggtggt
     1921 agcacttctt ctgagcatag gggttctgcg ggtggatctg ttttgtttcc tcgtaccggt
     1981 acagttcttc catgggtatt aacagggcca ggtgccgcag cagttagagc caggagcgaa
     2041 gctcttagga cacatttgag agcatctaca gaatggtctc ccgctggtgt tggccaagcc
     2101 ttactagcag gaactggggc aggagctgat actcatagag cagttgtatt ggcaggtgac
     2161 agggctcaaa cccttaatgc cctagcagca cttagtgcag gggcagacca tccggcggtg
     2221 ttcacgtcta cgcgtgcgga tgcctcacca gcaggtccag tatttgtttt ccctggtcaa
     2281 ggttctcaat ggacaggtat ggcaagagaa ctattagatt ctgcgcccgt tttcgcgagg
     2341 aagctgcacg actgtgccga tgccttcgct ccttacttgg gtcatagttt attggactca
     2401 gtcacaggag cggcaggtgg tcctgagccg gtgggcgctg acgtggttca acccgcattg
     2461 tttgcagtta tggtcgctct tacggattta tggaatgcag ctggtgttgc accaggtgct
     2521 cttttaggcc actctctagg cgaacttgct gccgcacacg ttgcgggtgt tttatcatta
     2581 gacgatagtg caagagtggt tgctagatgg tctcaagctc aagccacact agctggaaga
     2641 ggagacatgg taagtgtttt gttacccgct gatgagttag ctgatctact ggacagacgt
     2701 tggcccggca gattagttgt ggccgttgaa aatggaccgg ggtccgctgt cgcttcaggt
     2761 gatttggacg ctgcagctga gttggttgct catctgactg ctgaaggcat tcacgcgcgt
     2821 agagttgatg taggtctagc cgctcactct ccgcacattg atgcaatact gcccaggatc
     2881 agagctgata ttgcaccaat aagagcacac acaccatcta ttccagtata ctcagcattg
     2941 catggaggag cacttgacgg tactccaatg gacgcagcgt attggtgtag aaatttgcgt
     3001 tctacggtta gattcgctga cgctactaga gctgctctag aagctggtca cacaacattc
     3061 gtagaagtaa gcccacatcc cgtcttgacc actgccatgg aagtctcagc cacgagagcc
     3121 gcccatgctg ctacagtttt gggtactctt agaaggggtg agggtggacc ttccagattc
     3181 ttagcctcct tagccgaact acacgtgtcc ggtggagatg cagatttaag aactgttttg
     3241 ccggccagcc aagcagctgg gttacctgaa acagtgctaa cggctggccc gagaggtgaa
     3301 tcagcagatg gagacagtag acatgaagtc ctatgtgcaa gattagctcc tttagatcca
     3361 gctgaaagac gtgcccaatt attaaccgtt gttagagaga gtgccgccgc cgctttagat
     3421 ggcgacgatc agggttcaat tgacggaaga agaactttta gagatttagg tataacgtcc
     3481 ctggctgcag taggtatcag ggaccgtcta cactctgcca ccggtcttag actgtctcca
     3541 accgtggttt ttgatcatcc cactcctgac gcactagcag ctcatcttga caccgaatta
     3601 ttcgggactg gagctgacgc ggagcccgct ccagctgcag gtggtagagc ggtaccgcac
     3661 gatgaaccga ttgctatcgt cggcatggca tgcagatacc ccggtggcgt aggtgcacct
     3721 gctgatttat ggagaaccgt attagctggg gtcgatgctg tgggtccact tcctgcggat
     3781 agaggttgga acatcgccga tggttacgat ccggagcttg ccggtccggg taggttttca
     3841 caacgtgagg gtggattctt acatgacgct gcagaatttg acgcggagtt ttttggtata
     3901 tcaccaagag aggctttggc aatggatcca caacaaaggc tagctttgga gtctgcgtgg
     3961 gaagctattg aagacgcagg tttagacgcc cactcactga gagggtcccg tacgggcgta
     4021 tttttagggt tgatcaccca agattatggt cctagagccg gggaacccac gactcgtgct
     4081 ggtgctgtgg aaggacattt gtttttagga agcacaggat ctgttgcttc tggtagatta
     4141 tcttatactt tgggtctaga gggcccatca ttaacgatag acactgcttg ctcatcttct
     4201 ctggtagcat tgcatgaagc ttgtcaagct ctaaggactg gtgattgtga tatggctttg
     4261 acgggtgggg ttacggtcat gccatcaact ggtatgttag tggagttcag cagacaaagg
     4321 gggttatccc ccgacggcag gtgtaaagcg ttttccgcat cagctgatgg atttggtctg
     4381 gcagaagggg taggcatgtt ggtcgttgaa cgtttgtccg atgctagaag attgggccac
     4441 agggtattag ctgtagtaag aggttccgcc gtcaaccaag acggggcgtc taatggtcta
     4501 tctgctccta gcggtccggc gcagcaaaga gtcatcagac aagcattggt taatgcaggt
     4561 gtacaagcat cacaagttga tgttgtagag gcacatggta ctggtaccaa attaggggac
     4621 cctattgaag cgcaagcatt gcaggcaact tacggtcaag gccgtccagc tgaaagacct
     4681 ctgtggttgg ggagtcttaa gtctaacatc ggtcacgccc aggccgctgc tggcgtaggt
     4741 ggggtcataa agatggttat ggctttaaga gaaggcgttt tgcctcctac tttgcacgcc
     4801 gatgaaccgt caccccatat agattggtct gctggtcaag taagattgct taccgaggag
     4861 agagaatggc cggaagcagg ccatcctaga cgtgctgctg tatcttcatt tggtgtcagt
     4921 gggactaatg cccacgtgat cattgaagaa gctcccgaag aaggcgaaga accagaatca
     4981 gacgctgggg gggtcgtgcc atgggttcta tcagctagga ctgaaggagc attgcaagcg
     5041 caagccgtgc agttgagtga gtttgtgggt gaatcttctc ccgtcgacgt tggttggtcc
     5101 cttgtgagta ctcgtgctgc ttttgaacac agggctgttg ttgttgggag aggccgtgac
     5161 gaattggtga gaggcttatc cgaggttgca caaggccgtg gagttagagg tgttgcttct
     5221 tctgcttcag gtggtttggc gttcgttttc gcagggcaag gaagtcaacg tttgggtatg
     5281 ggcaggggtc tgtacgaaag gtttcccgtg ttcgctgaag cttttgacga ggtttgcggt
     5341 agagtgggtc ctggtgttag agaggttgtc tttggaagtg acgcgggtga attggaccgt
     5401 acagtttggg ctcaagctgg gctgttcgca ttagaagttg cgttgtttag attattggaa
     5461 agttggggag tcagacctgg atgtttaata ggccattctg ttggcgagtt atccgcagct
     5521 tgtgtggccg gtctttggag cctagaggat gcttgccgtg tagtcgccgc ccgtgctaga
     5581 ctgatgcagg cgttgccagc tggtggagtt atggttgcgg ttagggcaga ggcgggggag
     5641 ttagcaggct ttttaggaga agacgttgtg attgcatctg ttaatgctcc agggcaagtt
     5701 gttatagcag gtccggaggg cggtgttgaa agagttgttg ccgcgtgtgg cgctagatct
     5761 aggagattgg ctgtttcaca cgctttccat tctccgttag ttgagccaat gttgggtgag
     5821 tttagaagag tggttgaatc agtcgctttt ggtgttccat ccttgagagt agtttctaac
     5881 gtaacaggtg cttgggttga tccagaagag tggggtacac cagaatactg ggttagacaa
     5941 gtcagagaac cagtacgttt tgctgatggt gttgcgacat tgttagatgc gggtgtgaga
     6001 acattcgttg aactggggcc agctggtgcc ttgactagta tggtctcaca ttgcgctgac
     6061 gcgacagcaa cctctgtcac ggcagtgcca actttaagac ctgatcacga tgaatctagg
     6121 actgttttgt ccgctgcagc ttctttatat gttcaaggcc atccagttga ctgggccccg
     6181 cttttcccta gagctagaac ggtagatctg cctacttatg cattccaaca ccagagatat
     6241 tggctagata gtacaacggc caacactggg gatagacccg cggctgatag ggatactgct
     6301 ttctgggaag ctgttcaaca tacagacttg gatgcctttg ctgcagaact ggacattgca
     6361 ccggatgcgc ctttaggaac cgttctacca gctttggcag actggagaca acgtttgaga
     6421 acagctgctg cagtggatgc ttggagatac aggactgcat tcaaaagatt accggacgct
     6481 cctggtgctc cagttctgac cggtagttgg ttggctgtag taccagtcag acacttggac
     6541 gacccttctg tgacgacatc tttagacgcc gttgccaaag caggtgcaga agttgttcaa
     6601 ttagctattg aagacgctga tgctgacgta gacagactaa cagagaggtt gaggggttta
     6661 gtagccggat taggtgcagc cccagcgggt ataatgtcat tcctgggttt ggatgaggaa
     6721 agacataggg accatccagc gatgcccagc ggattagcta ctagtttagc tcttgttaga
     6781 gcactaggcc gtgccggtat tggtgctcct ttgtggatgg tcacgaggga ggctgtggct
     6841 gctggtcaag atacccaccc acacgcccca ttgggcagcc tgatttgggg tttagggcag
     6901 gtaacggctt tggagcatgc cgatagatgg ggaggtttaa ttgatctgcc cggggtttgc
     6961 gacgcccgtg tcgctagaat gctttgtgca gggctgagtg gcaggggtgc tgaagatcaa
     7021 cttgctttaa ggccttccgg tacgtttgtt aggagacttg cccatatccc aggggaacaa
     7081 cgtgctgcaa gaaggtcttg gcaaccaaga ggtactgtta ttgtgactgg tggtacaggt
     7141 gctttaggtg cggtgctggc cagatggctg gctactgaag atgctgaaca tttagttctg
     7201 accggaagaa gaggcgctga cgcaccaggt gcagagaggt tgagagatga attggttgca
     7261 accggtgcta gagtcacctt agcagcttgt gacgtagccg ataggaaagc tgtcgcagct
     7321 ctacttgatg aattggccgc agacggagaa actgttagag ctgttttgca tgccgcaggt
     7381 gtagcagatt tgacctcctt ggaaaatacc gggcccgaag cctttgccgc tggggtggct
     7441 gctaaggtcg acggggctct gcacttgact gaattactag atcatgactc tttggatgca
     7501 tttgtattgt tttcagccat cgctggggtt tggggcagcg gtgatcatgg tgcatatgca
     7561 gctgccaacg catttctaaa cgcattagct gagtataaca gggcaagagg tattccaacc
     7621 acctctattg cttggggtgt ttggaatgct tttggtgttg aaggtgccgg cggtattagt
     7681 gaggccgtcg atttagacca attacataga aggggtctgc ctttgataga acctgaatta
     7741 ggacttaccg ccctaagaag agcactggac agagacgaaa cagtattgac ggtcgcaccc
     7801 gttgcgtggg aaagattctt tccacttttt tcagctgcta gacctagacc cctatttgag
     7861 gatctacctc aagtaagagc cttatccgct cctgtaccaa caacagcagg gcctgctgtc
     7921 gaacctggta gaaggggttc tgggttgggt gatttacctc tgacagacag ggatagtgct
     7981 ttattagcac tagtccgtgg cgagagtgct agtgttttag gatatgaaag accagacaga
     8041 ctagatcctg atagagctct gcgtgacgtt ggcttcgata gtcttacagc aatggagcta
     8101 agaaatcgtt tggctacagc cactggtctg acactacctg cagctctggt gttcgaccac
     8161 ccgactccct tagccatcgc cgcatatcta aaggcagaat taaccagtca gctggactcc
     8221 ggtacgcctg ctagagaagc aagttccgct ttaagggacg gttatagaca agctggcgtt
     8281 tctggaagag ttaggagtta ccttgatctt ttggcaggct tatccgattt cagagaacat
     8341 tttgacggtt ctgacggttt ttctctagat ctagtagata tggcggatgg tccaggtgaa
     8401 gtaacggtta tatgttgcgc aggtacagcg gccattagcg gtcctcatga attcactaga
     8461 cttgcaggtg cattacgtgg tattgcccct gtcagagcgg tcccacaacc ggggtatgaa
     8521 gaaggcgaac cattgccatc atccatggcc gcggtggctg cagttcaggc agacgcagtg
     8581 atcaggacgc agggtgacaa accatttgtc gtcgccggcc attcagccgg tgctcttatg
     8641 gcttatgcct tggctacgga gttattagat agagggcatc ctccaagagg tgttgtccta
     8701 atagatgttt accccccagg acatcaggat gcgatgaacg cttggttaga agaactgaca
     8761 gcaactctgt ttgatagaga gacagttaga atggatgata caagattaac cgctttgggt
     8821 gcctacgacc gtcttacagg acaatggaga ccaagagaaa caggtttacc aaccttattg
     8881 gtttctgctg gtgagccaat ggggccatgg cctgatgatt cttggaagcc tacttggcca
     8941 ttcgaacatg atacggtcgc agttcctggc gaccatttta cgatggtaca agagcatgct
     9001 gatgcgatcg ccagacacat agatgcctgg ttaggtggtg gaaactcctg aagcttcgaa
     9061 tttacgtagc ccaatctacc actttttttt ttcatttttt aaagtgttat acttagttat
     9121 gctctaggat aatgaactac tttttttttt ttttttttac tgttatcata aatatatata
     9181 ccttattgtt gtttgcaacc gtcggttaat tccttatcaa ggttccccaa gttcggatca
     9241 ttaccatcaa tttccaacat tttcatgagt tcttcttctt cattaccgtg ttttaggggg
     9301 ctgttgcaac tcagaagttt gacagcaagc aagttcatca ttcgaactag ccttattgtt
     9361 ttagttcagt gacagcgaac tgccgtactc gatgctttat ttctcacggt agagcggaag
     9421 aacagatagg ggcagcgtga gaagagttag aaagtaaatt tttatcacgt ctgaagtatt
     9481 cttattcata ggaaattttg caaggttttt tagctcaata acgggctaag ttatataagg
     9541 tgttcacgcg attttcttgt tatgtatacc tcttctctga ggaatggtac tactgtcctg
     9601 atgtaggctc cttaaattgg tgggcaagaa taacttatcg atattttgta tattggtctt
     9661 ggagttcacc acgtaatgcc tgtttaagac catcagttaa ctctagtatt atttggtctt
     9721 ggctactggc cgtttgctat tattcaagtc ttttgtgcct tcccgtcggg taagggagtt
     9781 atttagggat acagaatcta acgaaaacta aatctcaatg attaactcta tttaatcctt
     9841 ttttgaaagg caaaagaggt cccttgttca cttacaacgt tcttagccaa attcgcttat
     9901 cacttactac ttcacgatat acagaagtaa aaacatataa aaagatgtct gtttgtttag
     9961 ccatcacaaa aggtatcgca gattaagacc tcagcgcggc cgcaaattta aataaaatga
    10021 agtgaagttc ctatactttc tagagaatag gaacttctat agtgagtcga ataagggcga
    10081 cacaaaattt attctaaatg cataataaat actgataaca tcttatagtt tgtattatat
    10141 tttgtattat cgttgacatg tataattttg atatcaaaaa ctgattttcc ctttattatt
    10201 ttcgagattt attttcttaa ttctctttaa caaactagaa atattgtata tacaaaaaat
    10261 cataaataat agatgaatag tttaattata ggtgttcatc aatcgaaaaa gcaacgtatc
    10321 ttatttaaag tgcgttgctt ttttctcatt tataaggtta aataattctc atatatcaag
    10381 caaagtgaca ggcgccctta aatattctga caaatgctct ttccctaaac tccccccata
    10441 aaaaaacccg ccgaagcggg tttttacgtt atttgcggat taacgattac tcgttatcag
    10501 aaccgcccag ggggcccgag cttaagactg gccgtcgttt tacaacacag aaagagtttg
    10561 tagaaacgca aaaaggccat ccgtcagggg ccttctgctt agtttgatgc ctggcagttc
    10621 cctactctcg ccttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10681 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10741 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10801 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10861 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10921 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10981 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    11041 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    11101 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    11161 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    11221 gaagtggtgg gctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    11281 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    11341 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    11401 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgcgcgtaac
    11461 tcacgttaag ggattttggt catgagcttg cgccgtcccg tcaagtcagc gtaatgctct
    11521 gcttttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    11581 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    11641 gccccagcgc tgcgatgata ccgcgagaac cacgctcacc ggctccggat ttatcagcaa
    11701 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    11761 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    11821 gcaacgttgt tgccatcgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    11881 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    11941 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    12001 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    12061 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    12121 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    12181 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    12241 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    12301 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    12361 cgacacggaa atgttgaata ctcatattct tcctttttca atattattga agcatttatc
    12421 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    12481 gggtcagtgt tacaaccaat taaccaattc tgaacattat cgcgagccca tttatacctg
    12541 aatatggctc ataacacccc ttgtttgcct ggcggcagta gcgcggtggt cccacctgac
    12601 cccatgccga actcagaagt gaaacgccgt agcgccgatg gtagtgtggg gactccccat
    12661 gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
    12721 ctttcgcccg ggctaattat ggggtgtcgc ccttattcga ctctatagtg aagttcctat
    12781 tctctagaaa gtataggaac ttctgaagtg gggatttaaa tgcggccgcg ctgagggttt
    12841 aat
//
