LOCUS       sequence-325287-        7463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for integrating minCMV promoter-driven dual surface 
            marker/fluorescent reporter at AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van 
            MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik 
            MC, Bintu L
  TITLE     High-Throughput Discovery and Characterization of Human 
            Transcriptional Effectors.
  JOURNAL   Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 
            10.1016/j.cell.2020.11.024.
  PUBMED    33326746
REFERENCE   2  (bases 1 to 7463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024."
COMMENT     Sequence Label: pDY32
            AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG...
FEATURES             Location/Qualifiers
     source          1..7463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     149..166
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    283..304
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(319..324,325..342,343..349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    357..373
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     362..384
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     381..397
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     381..397
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        418..436
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    471..1274
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1281..1306
                     /label=SA
                     /note="splice acceptor site"
     CDS             1330..1383
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1393..1992
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1393..1412)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1889..1909
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2024..2041)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2030..2254
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2327..2345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2363..2381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2399..2417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2435..2453
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2471..2489
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2507..2525
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2543..2561
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2579..2597
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2615..2633
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2671..2708
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2705..2729
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2742..2751
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     2748..2807
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             3579..3608
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3612..3758
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             3774..3827
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      3834..3843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3840..3842,3843..3845,3846..4556)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3885..3906)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4146..4165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4493..4514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4557..4577
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4588..4709
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4625..4644)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4679..4698
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    4775..5611
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(5886..6677,6678..6746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6509..6528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6870..7458
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7359..7378
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
       61 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      121 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      181 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      241 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      301 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      361 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctcagaat
      421 taaccctcac taaagggact agtcctgcag gtttaaacga attcgccctt tgctttctct
      481 gaccagcatt ctctcccctg ggcctgtgcc gctttctgtc tgcagcttgt ggcctgggtc
      541 acctctacgg ctggcccaga tccttccctg ccgcctcctt caggttccgt cttcctccac
      601 tccctcttcc ccttgctctc tgctgtgttg ctgcccaagg atgctctttc cggagcactt
      661 ccttctcggc gctgcaccac gtgatgtcct ctgagcggat cctccccgtg tctgggtcct
      721 ctccgggcat ctctcctccc tcacccaacc ccatgccgtc ttcactcgct gggttccctt
      781 ttccttctcc ttctggggcc tgtgccatct ctcgtttctt aggatggcct tctccgacgg
      841 atgtctccct tgcgtcccgc ctccccttct tgtaggcctg catcatcacc gtttttctgg
      901 acaaccccaa agtaccccgt ctccctggct ttagccacct ctccatcctc ttgctttctt
      961 tgcctggaca ccccgttctc ctgtggattc gggtcacctc tcactccttt catttgggca
     1021 gctcccctac cccccttacc tctctagtct gtgctagctc ttccagcccc ctgtcatggc
     1081 atcttccagg ggtccgagag ctcagctagt cttcttcctc caacccgggc ccctatgtcc
     1141 acttcaggac agcatgtttg ctgcctccag ggatcctgtg tccccgagct gggaccacct
     1201 tatattccca gggccggtta atgtggctct ggttctgggt acttttatct gtcccctcca
     1261 ccccacagtg gggcaagctt ctgacctctt ctcttcctcc cacagggcct cgagagatct
     1321 ggcagcggag agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc
     1381 cctaggctcg agatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1441 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1501 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1561 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg
     1621 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1681 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     1741 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     1801 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     1861 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1921 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1981 cccggtgcct gatctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2041 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2101 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2161 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2221 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2281 gggctagcaa agcctaggaa cagctgcata tagcactgca gtttactccc tatcagtgat
     2341 agagagtcaa aaggccatgc cctccctatc agtgatagag agagcagact caaggtggtc
     2401 cctatcagtg atagagagat tgagcaagag ggcctcccta tcagtgatag agaatagcac
     2461 tgcagtttac tccctatcag tgatagagag tcaaaaggcc atgccctccc tatcagtgat
     2521 agagagagca gactcaaggt ggtccctatc agtgatagag agattgagca agagggcctc
     2581 cctatcagtg atagagattg atccagtcag ggcctcccta tcagtgatag agaagctatg
     2641 tgcaggtagg gctgtacagt gggaatgcat gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcc caccgcggtg ggccaccatg gagacagaca
     2761 cactcctgct atgggtactg ctgctctggg ttccaggttc cactggtgac gctagcgaga
     2821 cgggcggatc aggcggtcgt ctcggatccg acaaaactca cacatgccca ccgtgcccag
     2881 cacctgaact cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc
     2941 tcatgatctc ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc
     3001 ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc
     3061 cgcgggagga gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc
     3121 aggactggct gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc
     3181 ccatcgagaa aaccatctcc aaagccaaag ggcagccccg agaaccacag gtctacaccc
     3241 tgcccccatc ccgggaggag atgaccaaga accaggtcag cctgacctgc ctggtcaaag
     3301 gcttctatcc cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact
     3361 acaagaccac gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca
     3421 ccgtggacaa gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcacgagg
     3481 ctctgcacaa ccactacacg cagaagagcc tctccctgtc tccgggtaaa ggaggaggtg
     3541 gaagcggagg aggaggaagc ggaggaggag gtagcggaga acaaaaactc atctcagaag
     3601 aggatctgaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca cactccttgc
     3661 cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc atcatctccc
     3721 ttatcatcct catcatgctt tggcagaaga agccacgttg tacaggcagt ggagagggca
     3781 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccaccg gtcgccacca
     3841 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     3901 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     3961 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     4021 tcgtgaccac cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc
     4081 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     4141 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     4201 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     4261 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     4321 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     4381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     4441 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     4501 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagccaa
     4561 aaaagaagag aaaggtatag ttaactaaac ttgtttattg cagcttataa tggttacaaa
     4621 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4681 ggtttgtcca aactcatcaa tgtatcttat catgtctgca tatgtaaatt gtaagcgtct
     4741 cggcctctag agtcgagatg acattgatta ttgaactagg gacaggattg gtgacagaaa
     4801 agccccatcc ttaggcctcc tccttcctag tctcctgata ttgggtctaa cccccacctc
     4861 ctgttaggca gattccttat ctggtgacac acccccattt cctggagcca tctctctcct
     4921 tgccagaacc tctaaggttt gcttacgatg gagccagaga ggatcctggg agggagagct
     4981 tggcaggggg tgggagggaa gggggggatg cgtgacctgc ccggttctca gtggccaccc
     5041 tgcgctaccc tctcccagaa cctgagctgc tctgacgcgg ctgtctggtg cgtttcactg
     5101 atcctggtgc tgcagcttcc ttacacttcc caagaggaga agcagtttgg aaaaacaaaa
     5161 tcagaataag ttggtcctga gttctaactt tggctcttca cctttctagt ccccaattta
     5221 tattgttcct ccgtgcgtca gttttacctg tgagataagg ccagtagcca gccccgtcct
     5281 ggcagggctg tggtgaggag gggggtgtcc gtgtggaaaa ctccctttgt gagaatggtg
     5341 cgtcctaggt gttcaccagg tcgtggccgc ctctactccc tttctctttc tccatccttc
     5401 tttccttaaa gagtccccag tgctatctgg gacatattcc tccgcccaga gcagggtccc
     5461 gcttccctaa ggccctgctc tgggcttctg ggtttgagtc cttggcaagc ccaggagagg
     5521 cgctcaggct tccctgtccc ccttcctcgt ccaccatctc atgcccctgg ctctcctgcc
     5581 ccttccctac aggggttcct ggctctgctc ttcgccttct tgacgagttc ttctgaatta
     5641 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     5701 accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     5761 taaatacatt caaatatgta tccgctcatg agattatcaa aaaggatctt cacctagatc
     5821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6421 ttttctgtga ctggtgaata ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6781 cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6841 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6901 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6961 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     7021 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     7081 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     7141 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     7201 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     7261 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     7321 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     7381 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7441 gggcggagcc tatggaaaaa cgc
//
