LOCUS       sequence-325288-       10511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus 
            with a tagBFP fluorescent marker linked by a self-cleaving P2A 
            peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Abatti LE, Lado-Fernandez P, Huynh L, Collado M, Hoffman MM, 
            Mitchell JA
  TITLE     Epigenetic reprogramming of a distal developmental enhancer cluster 
            drives SOX2 overexpression in breast and lung adenocarcinoma.
  JOURNAL   Nucleic Acids Res. 2023 Sep 22:gkad734. doi: 10.1093/nar/gkad734.
  PUBMED    37738673
REFERENCE   2  (bases 1 to 10511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Sep 22:gkad734. doi: 10.1093/nar/gkad734."
COMMENT     Sequence Label: AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP
FEATURES             Location/Qualifiers
     source          1..10511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(185..253,254..1045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(403..422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1216..1804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1705..1724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1981..2510
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     protein_bind    2704..2722
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2740..2758
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2776..2794
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2812..2830
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2848..2866
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2884..2902
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2920..2938
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2983..3007
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4056..4082
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4086..4112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4116..4142
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4152..4208
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4209..4907
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    4936..5057
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4973..4992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5027..5046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5106..5122)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5106..5122)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5119..5141)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5130..5146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5154..5160,5161..5178,5179..5184))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5199..5220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5337..5354)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5410..5443
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5774..5793
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5792..5847
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5893..5912
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6003..6749)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6781..6800)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6808..7824
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6862..6884)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(7825..8102)
                     /label=chicken beta-actin promoter
     enhancer        8104..8483
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    8489..8713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8702..8719
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8739..9542)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8850..8869)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9460..9479
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     regulatory      9539..9548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(9564..9617)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    9641..9666
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    9673..9706
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9708..10511
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 aagggcgaat tcaaccctga taaatgcttc aataatcaga tcttccggat ggctcgagtt
       61 tttcagcaag ataaatgctt caataatggg gccactaggg acaggattgg attgaaaaag
      121 gaagaatctt tctagaagat ctcctacaat attctcagct gccatggatt gaaaaaggaa
      181 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      241 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      301 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      361 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      421 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      481 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      541 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      601 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      661 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      721 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      781 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      841 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      901 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      961 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     1021 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1081 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1141 catgaccaaa atcccttaac gggagttttc gttccactga gcgtcagacc ccgtagaaaa
     1201 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1261 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1321 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1381 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1441 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1501 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1561 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1621 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1681 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1741 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1801 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcg aattcgccct tagagcagag
     1861 ccaggaaccc ctgtagggaa ggggcaggag agccaggggc atgagatggt ggacgaggaa
     1921 gggggacagg gaagcctgag cgcctctcct gggcttgcca aggactcaaa cccagaagcc
     1981 cagagcaggg ccttagggaa gcgggaccct gctctgggcg gaggaatatg tcccagatag
     2041 cactggggac tctttaagga aagaaggatg gagaaagaga aagggagtag aggcggccac
     2101 gacctggtga acacctagga cgcaccattc tcacaaaggg agttttccac acggacaccc
     2161 ccctcctcac cacagccctg ccaggacggg gctggctact ggccttatct cacaggtaaa
     2221 actgacgcac ggaggaacaa tataaattgg ggactagaaa ggtgaagagc caaagttaga
     2281 actcaggacc aacttattct gattttgttt ttccaaactg cttctcctct tgggaagtgt
     2341 aaggaagctg cagcaccagg atcagtgaaa cgcaccagac agccgcgtca gagcagctca
     2401 ggttctggga gagggtagcg cagggtggcc actgagaacc gggcaggtca cgcatccccc
     2461 ccttccctcc caccccctgc caagctctcc ctcccaggat cctctctggc tccatcgtaa
     2521 gcaaacctta gaggttctgg caaggagaga gatggctcca ggaaatgggg gtgtgtcacc
     2581 agataaggaa tctgcctaac aggaggtggg ggttagaccc aatatcagga gactaggaag
     2641 gaggaggcct aaggatgggg cttttctgtc aaaagctgat atctccaccc cacagtggtt
     2701 tactccctat cagtgataga gaacgtatga agagtttact ccctatcagt gatagagaac
     2761 gtatgcagac tttactccct atcagtgata gagaacgtat aaggagttta ctccctatca
     2821 gtgatagaga acgtatgacc agtttactcc ctatcagtga tagagaacgt atctacagtt
     2881 tactccctat cagtgataga gaacgtatat ccagtttact ccctatcagt gatagagaac
     2941 gtataagctt taggcgtgta cggtgggcgc ctataaaagc agagctcgtt tagtgaaccg
     3001 tcagatcgcc tggagcaatt ccacaacact tttgtcttat accaactttc cgtaccactt
     3061 cctaccctcg taaacttaag gccaccatgt acaacatgat ggagacggag ctgaagccgc
     3121 cgggcccgca gcaaacttcg gggggcggcg gcggcaactc caccgcggcg gcggccggcg
     3181 gcaaccagaa aaacagcccg gaccgcgtca agcggcccat gaatgccttc atggtgtggt
     3241 cccgcgggca gcggcgcaag atggcccagg agaaccccaa gatgcacaac tcggagatca
     3301 gcaagcgcct gggcgccgag tggaaacttt tgtcggagac ggagaagcgg ccgttcatcg
     3361 acgaggctaa gcggctgcga gcgctgcaca tgaaggagca cccggattat aaataccggc
     3421 cccggcggaa aaccaagacg ctcatgaaga aggataagta cacgctgccc ggcgggctgc
     3481 tggcccccgg cggcaatagc atggcgagcg gggtcggggt gggcgccggc ctgggcgcgg
     3541 gcgtgaacca gcgcatggac agttacgcgc acatgaacgg ctggagcaac ggcagctaca
     3601 gcatgatgca ggaccagctg ggctacccgc agcacccggg cctcaatgcg cacggcgcag
     3661 cgcagatgca gcccatgcac cgctacgacg tgagcgccct gcagtacaac tccatgacca
     3721 gctcgcagac ctacatgaac ggctcgccca cctacagcat gtcctactcg cagcagggca
     3781 cccctggcat ggctcttggc tccatgggtt cggtggtcaa gtccgaggcc agctccagcc
     3841 cccctgtggt tacctcttcc tcccactcca gggcgccctg ccaggccggg gacctccggg
     3901 acatgatcag catgtatctc cccggcgccg aggtgccgga acccgccgcc cccagcagac
     3961 ttcacatgtc ccagcactac cagagcggcc cggtgcccgg cacggccatt aacggcacac
     4021 tgcccctctc acacatgggt agcggcagcg gtagctaccc gtatgatgtt ccggattacg
     4081 ctggctatcc ctacgacgtg cccgactatg ccgggtaccc ctatgacgtc ccagactacg
     4141 caggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg gaggagaacc
     4201 ctggacctag cgagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg
     4261 tggacaacca tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc
     4321 agaccatgag aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg
     4381 ctactagctt cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact
     4441 tcttcaagca gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg
     4501 ggggcgtgct gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg
     4561 tcaagatcag aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg
     4621 gctgggaggc cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg
     4681 acatggccct gaagctcgtg ggcgggagcc atctgatcgc aaacatcaag accacatata
     4741 gatccaagaa acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac
     4801 tggaaagaat caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg
     4861 ccagatactg cgacctccct agcaaactgg ggcacaagct taattagtaa gcggccgcaa
     4921 tcgatcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4981 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5041 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     5101 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5161 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5221 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5281 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     5341 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     5401 gacggccgca taacttcgta taatgtatgc tatacgaagt tataagcttg ggctgcaggt
     5461 cgagggatct ccataagaga agagggacag ctatgactgg gagtagtcag gagaggagga
     5521 aaaatctggc tagtaaaaca tgtaaggaaa attttaggga tgttaaagaa aaaaataaca
     5581 caaaacaaaa tataaaaaaa atctaacctc aagtcaaggc ttttctatgg aataaggaat
     5641 ggacagcagg gggctgtttc atatactgat gacctcttta tagccaacct ttgttcatgg
     5701 cagccagcat atgggcatat gttgccaaac tctaaaccaa atactcattc tgatgtttta
     5761 aatgatttgc cctcccatat gtccttccga gtgagagaca caaaaaattc caacacacta
     5821 ttgcaatgaa aataaatttc ctttattagc cagaagtcag atgctcaagg ggcttcatga
     5881 tgtccccata atttttggca gagggaaaaa gatctcagtg gtatttgtga gccagggcat
     5941 tggccacacc agccaccacc ttctgatagg cagcctgcac ctgaggagtg aattcacgcg
     6001 ttttagcctg gcagcatatc gagatcgaag tcatcgagag cgtcagcagg gagcatatcc
     6061 agatcaaaat catccagggc atcggcgggc agcatgtcca ggtcgaagtc gtccagggcg
     6121 tcggtgggtc cgccgctctc gcacttcagc tgcttctcga ggccgcagat gatcagttcc
     6181 aggccgaaca ggaaggcggg ctcggcgccc tgtctgtcga acagctcgat ggcctgcttc
     6241 agcagggggg gcatgctgtc ggtggtgggt gtctctcttt cctctttggc gacctggtgt
     6301 tcctgttctt ccagcacgca gcccagggtg aagtggccca cggcgctcag ggcgtacagg
     6361 gcgttttcca ggctgaagcc ctgctggcac aggaaggcca gctggttttc cagtgtctcg
     6421 tactgcttct cggtgggtct ggtgcccagg tgcactttgg cgccgtcccg gtggctcagc
     6481 agggcgcatc tgtagctctt ggcgttgttc cgcaggaagt cctgccagct ctcgccttcc
     6541 agagggcagc tgtgggtgtg gtgccggtcc agcatctcga tgggcagggc gtccagcagg
     6601 gcccgcttgt tcttcacgtg ccagtacagg gtgggctgtt ccacgcccag tttctgggcc
     6661 agcttccggg tggtcaggcc ctcgatgccc acgccgttca gcagttccag ggcgctgttg
     6721 atcactttgc tcttgtccag ccggctcatg tcgacattct ttgccaaaat gatgagacag
     6781 cacaataacc agcacgttgc ccaggagctg taggaaaaag aagaaggcat gaacatggtt
     6841 agcagaggct ctagagccgc cggtcacacg ccagaagccg aaccccgccc tgccccgtcc
     6901 cccccgaagg cagccgtccc cccgcggaca gccccgaggc tggagaggga gaaggggacg
     6961 gcggcgcggc gacgcacgaa ggccctcccc gcccatttcc ttcctgccgg cgccgcaccg
     7021 cttcgccccg cgcccgctag agggggtgcg gcggcgcctc ccagatttcg gctccgcaca
     7081 gatttgggac aaaggaagtc cctgcgccct ctcgcacgat taccataaaa ggcaatggct
     7141 gcggctcgcc gcgcctcgac agccgccggc gctccggggg ccgccgcgcc cctcccccga
     7201 gcccyccccg gcccgaggcg gccccgcccc gcccggcacc cccacctgcc gccacccccc
     7261 gcccggcacg gcgagccccg cgccacgccc cgtacggagc cccgcacccg aagccgggcc
     7321 gtgctcagca actcggggag gggggtgcag ggggggttgc agcccgaccg acgcgcccac
     7381 accccctgct caccccccca cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc
     7441 ccccccgcac cgcggggcac cgcccccggc cgcgctcccc tcgcgcacac tgcggagcgc
     7501 acaaagcccc gcgccgcgcc cgcagcgctc acagccgccg ggcagcgcgg agccgcacgc
     7561 ggcgctcccc acgcacacac acacgcacgc accccccgag ccgctccccc cgcacaaagg
     7621 gccctcccgg agcccctcaa ggctttcacg cagccacaga aaagaaacaa gccgtcatta
     7681 aaccaagcgc taattacagc ccggaggaga agggccgtcc cgcccgctca cctgtgggag
     7741 taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg
     7801 gcgaaggcag cgcgcagcga ctcccgcccg ccgcgcgctt cgctttttat agggccgccg
     7861 ccgccgccgc ctcgccataa aaggaaactt tcggagcgcg ccgctctgat tggctgccgc
     7921 cgcacctctc cgcctcsccc csccccsccc cccsccccsc cccsccccsc cyggcgcgcg
     7981 cccccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa
     8041 aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc
     8101 gaccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     8161 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     8221 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     8281 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     8341 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     8401 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     8461 ataactagtc aataatcaat gtcgagaccc atagagccca ccgcatcccc agcatgcctg
     8521 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
     8581 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
     8641 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
     8701 ctagaaggca cagtcgaggc tgatcagcgg gtttaaactc agaagaactc gtcaagaagg
     8761 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     8821 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     8881 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     8941 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcatc gccgtcgggc
     9001 atgcgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     9061 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     9121 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     9181 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     9241 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     9301 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ctgcagttca
     9361 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     9421 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     9481 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat ggccgatccc
     9541 atggtggcgg taccctcgag cctagggccg ggattctcct ccacgtcacc gcatgttaga
     9601 agacttcctc tgccctctcc gctgccagat ctctcgaggc cctgtgggag gaagagaaga
     9661 ggtcagaagc ttataacttc gtataatgta tgctatacga agttattgcc ccactgtggg
     9721 gtggagggga cagataaaag tacccagaac cagagccaca ttaaccggcc ctgggaatat
     9781 aaggtggtcc cagctcgggg acacaggatc cctggaggca gcaaacatgc tgtcctgaag
     9841 tggacatagg ggcccgggtt ggaggaagaa gactagctga gctctcggac ccctggaaga
     9901 tgccatgaca gggggctgga agagctagca cagactagag aggtaagggg ggtaggggag
     9961 ctgcccaaat gaaaggagtg agaggtgacc cgaatccaca ggagaacggg gtgtccaggc
    10021 aaagaaagca agaggatgga gaggtggcta aagccaggga gacggggtac tttggggttg
    10081 tccagaaaaa cggtgatgat gcaggcctac aagaagggga ggcgggacgc aagggagaca
    10141 tccgtcggag aaggccatcc taagaaacga gagatggcac aggccccaga aggagaagga
    10201 aaagggaacc cagcgagtga agacggcatg gggttgggtg agggaggaga gatgcccgga
    10261 gaggacccag acacggggag gatccgctca gaggacatca cgtggtgcag cgccgagaag
    10321 gaagtgctcc ggaaagagca tccttgggca gcaacacagc agagagcaag gggaagaggg
    10381 agtggaggaa gacggaacct gaaggaggcg gcagggaagg atctgggcca gccgtagagg
    10441 tgacccaggc cacaagctgc agacagaaag cggcacaggc ccaggggaga gaatgctggt
    10501 cagagaaagc a
//
