LOCUS       sequence-32529-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pET23(a)_mod
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             82..105
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             179..196
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(249..267)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      263..310
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(434..453)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatggcta gcatgactgg tggacagcaa
       61 atgggtcgcg gatcgaccgg tgactacaag gacgacgatg acaagtaccc ttatgacgtg
      121 cccgattacg ctggatccga attcgagctc cgtcgacaag cttgcggccg cactcgagca
      181 ccaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
      241 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
      301 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
      361 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
      421 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
      481 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
      541 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
      601 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
      661 actg
//
