LOCUS       sequence-325321-       10475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10475)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtPIP5:T35s
FEATURES             Location/Qualifiers
     source          1..10475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        198..302
                     /gene="bla"
                     /label=AmpR promoter
     CDS             303..1097
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      1268..1856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1757..1776
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    1979..2003
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      4256..4508
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    6082..6256
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        6356..6535
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             6557..7351
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6611..6630)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7221..7240
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      7364..7616
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7711..7735
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7999..8628
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9057..10130
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10196..10390
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
ORIGIN
        1 cggcctatcg cggccgctgg ccgctcaaaa atggctggcc tacggccagg caatctacca
       61 gggcgcggac aagccgcgcc gtcgccactc gaccgccggc gcccacatca aggcaccgag
      121 ctcccatccc cccaggggct gcgcccctcg gccgcgaacg gcctcacccc aaaaatggca
      181 gcgctggcca attcgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      241 tgtatccgct catgagacaa taaccttgat aaatgcttca ataatattga aaaaggaaga
      301 gtatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa taccgctgcg
      361 caaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga gaaaatgaaa
      421 acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat gtggaacggg
      481 aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc ctgcactttg
      541 aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc ctttgctcgg
      601 aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg gagtgcatca
      661 ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta gacagccgct
      721 tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc gaaaactggg
      781 aagaggacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag acggaaaagc
      841 ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc tttgtgaaag
      901 atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac aagtggtatg
      961 acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag tatgtcgagc
     1021 tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat tatattttac
     1081 tggatgaatt gttttagctg tcagaccaag tttactcata tatactttag attgatttaa
     1141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     1201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     1261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     1321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     1381 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     1441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     1501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     1561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1861 ccagcaacgc ggccttttta cggttcctgt ggttggcaca tacaaatgga cgaacggata
     1921 aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt tctcttaggt
     1981 ttacccgcca atatatcctg tcaaacactg atagtttaaa ctccctcggt cacatgtgca
     2041 tcctcgtaag gaggacgtcg ttgtggttgg tgctttcctt acattctgag cctctttcct
     2101 tctaatccac tcatctgcat cttcttgtgt ccttactaat acctcattgg ttccaaattc
     2161 cctcccttta agcaccagct cgtttctgtt cttccacagc ctcccaagta tccaagggac
     2221 taaagcctcc acattcttca gatcaggata ttcttgttta agatgttgaa ctctatggag
     2281 gtttgtatga actgatgatc taggaccgga taagttccct tcttcatagc gaacttattc
     2341 aaagaatgtt ttgtgtatca ttcttgttac attgttatta atgaaaaaat attattggtc
     2401 attggactga acacgagtgt taaatatgga ccaggcccca aataagatcc attgatatat
     2461 gaattaaata acaagaataa atcgagtcac caaaccactt gcctttttta acgagacttg
     2521 ttcaccaact tgatacaaaa gtcattatcc tatgcaaatc aataatcata caaaaatatc
     2581 caataacact aaaaaaatta aaagaaatgg ataatttcac aatatgttat acgataaaga
     2641 agttactttt ccaagaaatt cactgatttt ataagcccac ttgcattaga taaatggcaa
     2701 aaaaaaacaa aaaggaaaag aaataaagca cgaagaattc tagaaaatac gaaatacgct
     2761 tcaatgcagt gggacccacg gttcaattat tgccaatttt cagctccacc gtatatttaa
     2821 aaaataaaac gataatgcta aaaaaatata aatcgtaacg atcgttaaat ctcaacggct
     2881 ggatcttatg acgaccgtta aggaaattgt ggttgtcgga cgaagtccag taataaacgg
     2941 cgtcaaagtg gttgcagccg gcacacacga gtcgtgttta tcaactcaaa gcacaaatac
     3001 ttttcctcaa cctaaaaata aggcaattag ccaaaaacaa ctttgcgtgt aaacaacgct
     3061 caatacacgt gtcattttat tattagctat tgcttcaccg ccttagcttt ctcgtgacct
     3121 agtcgtcctc gtcttttctt cttcttcttc tataaaacaa tacccaaaga gctcttcttc
     3181 ttcacaattc agatttcaat ttctcaaaat cttaaaaact ttctctcaat tctctctacc
     3241 gtgatcaagg taaatttctg tgttccttat tctctcaaaa tcttcgattt tgttttcgtt
     3301 cgatcccaat ttcgtatatg ttctttggtt tagattctgt taatcttaga tcgtagacga
     3361 ttttctgggt ttgatcgtta gatatcatct taattctcga ttagggtttc atagatatca
     3421 tccgatttgt tcaaataatt tgagttttgt cgaataatta ctcttcgatt tgtgatttct
     3481 atctagatct ggtgttagtt tctagtttgt gcgatcgaat ttgtcgatta atctgagttt
     3541 ttctgattaa cagatgcaga tcttaatggg gtcatccaag aatgttatca aggagttcat
     3601 gaggtttaag gttcgcatgg aaggaacggt caatgggcac gagtttgaaa tagaaggcga
     3661 aggagagggg aggccatacg aaggccacaa taccgtaaag cttaaggtaa ccaagggggg
     3721 acctttgcca tttgcttggg atattttgtc accacaattt cagtatggaa gcaaggtata
     3781 tgtcaagcac cctgccgaca taccagacta taaaaagctg tcatttcctg aaggatttaa
     3841 atgggaaagg gtcatgaact ttgaagatgg tggcgtcgtt actgtaaccc aggattccag
     3901 tttgcaggat ggctgtttca tctacaaggt caagttcatt ggcgtgaact ttccttccga
     3961 tggacctgtt atgcaaaaga aaacaatggg ctgggaagcc agcactgagc gtttgtatcc
     4021 tcgtgatggc gtgttgaaag gagagattca taaggctctg aagctgaaag acggtggtca
     4081 ttacctagtt gaattcaaaa gtatttacat ggcaaagaag cctgtgcagc taccagggta
     4141 ctactatgtt gactccaaac tggatataac aagccacaac gaagattata caatcgttga
     4201 gcagtatgaa agaaccgagg gacgccacca tctgttcctt taagcttgtc aagcagatcg
     4261 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     4321 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     4381 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     4441 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     4501 actagatcga cgctgagctc gaattctagt ggagggagag aggattttga ggaaataatt
     4561 aattgaatta cttgtattat tgataaagta atttagataa gttgttagta caacttattg
     4621 caactatgga tagacaaaaa tcacttattt taaggggaga agtatataac aacttattat
     4681 aaaatttctg agcagccggc ctcctccaat caatcataga gagtggagcc cattctggaa
     4741 aaccaaggaa cccccaactt gcactggtgc ggtggcccaa ttcaaaaaaa acggggccca
     4801 gcaaagtaac gccgttgtaa cgatttatca atccaaactc aaaaggcggc gagatgcgtt
     4861 taactccgtg aaattaacaa accgccaaca acttgcaatt tgcaactacc gtttccaaga
     4921 agaactcaac cacacaacgt atcctatccc aaaccacacg caactagtga cgcgtcataa
     4981 ggacacgtgt cacaatttga ctggttaata atttcaccgc ttttgctata aattacctcc
     5041 aatcccctta gcttcttcac aattcagttc ccaaccctaa caattcttgt tcatatcgct
     5101 tctctctact ttcaaggtat gatccaattt ctctcttctt tctctgtaat cctttcgttg
     5161 agttttgttt ccgatcaatc ataggtagtt ttcttgtttc gaagcatgag atctaggaat
     5221 tttttgtgat tttccaaaat tgagatcggt ttgaaattga attttacagc ttgaatctca
     5281 gatcttgttt tatcaatgtt ttcgatggct tgcgatgtag atctatgata attgtggttc
     5341 agttttgtta ggaatcgatt tcggtttagc aattgcagat taattagggt ttccaattga
     5401 attcttcaga tccgttatgg aattatgtca aataatttat tcaaattgga aattattgtt
     5461 agatccactc ttaatctgtt tgatccaagc ttcaattagg gttttcactt gtttcaattt
     5521 cttgttatgg attctgattt atctgttgat gttagatcca ctcttaatct gtttgatcca
     5581 agcgttaatt agggttttca cttgtttcaa tttcgtgtgt tggattctga tttatctgtt
     5641 gttgatgtga ttacagaatg aaaagcacaa tccataaaac tgaggcatta ttagttctct
     5701 tcatgttgat gaactatgct tttgtgatct tagaagcatc acgtccattg agtatcattg
     5761 acaccaaaaa ctcagctact agagaggaag tcttggattt gttcgattgg ttatcacatt
     5821 ctcttggagc aatcaaacag ggtgtgccaa ttgaaacact cggagggatc aaggattcgg
     5881 gtccaagttc cggaggccca ggacacaaat ttaccgacag tgaaacactc ggagggatta
     5941 aggattcggg tccaagttcc ggaggcgtgg gacacaaatt taccaacagt gagactcttg
     6001 gtggaattaa ggactctggt ccatcacctg gccaaggaca taaataagct tctctagcta
     6061 gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg
     6121 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     6181 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     6241 tactaaaatc cagatcgcta agcttgaatt cttgcggaga gcggagaatt aagggagtca
     6301 cgttatgacc cccgccgatg acgcgggaca agccgtttta cgtttggaac tgacagaacc
     6361 gcaacgttga aggagccact cagccgcggg tttctggagt ttaatgagct aagcacatac
     6421 gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt cactatcagc tagcaaatat
     6481 ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc ctcggtatcc aattagagtc
     6541 tcatattcac tctcaaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6601 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6661 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     6721 tgccctgaat gaactccaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     6781 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     6841 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     6901 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     6961 ccaagcgaaa catcgcatcg agcgaggacg tactcggatg gaagccggtc ttgtcgatca
     7021 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7081 ggcgcggatg cccgacggcg aggatctcgt cgtgacccag ggcgatgcct gcttgccgaa
     7141 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7201 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7261 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7321 cttctatcgc cttcttgacg agttcttctg agcttgtcaa gcagatcgtt caaacatttg
     7381 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     7441 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     7501 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     7561 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg
     7621 ctggatccga attcggcagg gcccactgca tccaccccag tacattaaaa acgtccgcaa
     7681 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     7741 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     7801 atcagccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
     7861 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
     7921 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
     7981 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
     8041 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
     8101 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
     8161 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
     8221 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
     8281 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
     8341 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
     8401 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
     8461 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
     8521 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
     8581 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
     8641 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
     8701 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
     8761 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
     8821 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
     8881 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
     8941 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
     9001 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
     9061 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
     9121 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
     9181 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
     9241 ccggtgcgtc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
     9301 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
     9361 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
     9421 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
     9481 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
     9541 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
     9601 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
     9661 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
     9721 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
     9781 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
     9841 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
     9901 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
     9961 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
    10021 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
    10081 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
    10141 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaagcttc tttcctgtgg
    10201 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
    10261 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
    10321 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
    10381 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
    10441 ggtcgctgcg ctccctacgc cccgccgctt cgcgt
//
