LOCUS       sequence-325327-        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Smo-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: PD22
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      297..885
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1039..1056
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1296..1317
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1332..1337,1338..1355,1356..1362)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1370..1386
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1375..1397
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1394..1410
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1394..1410
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1460..1479
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             1671..2304
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2351..2476
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2973..3206
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3391..3435
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3731..3848
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        3900..4203
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4204..4407
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4358..4378
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4404..4428
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6879..6888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6888..6953
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(6979..7552,7553..7555,7556..7564)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7145..7162)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7372..7391
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7565..7963
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7977..8565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8030..8050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8448..8459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8713..8893
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8940..8958)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9026..9130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9131..9199,9200..9865,1..126)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9349..9368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
       61 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      121 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
      181 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
      241 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      301 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      361 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      421 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      481 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
      541 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
      601 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
      661 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
      721 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
      781 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
      841 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
      901 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
      961 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     1021 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     1081 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcaagctcat ggctgactaa
     1141 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     1201 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct ccccgtggca cgacaggttt
     1261 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     1321 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     1381 taacaatttc acacaggaaa cagctatgac atgattacga atttcacaaa taaagcattt
     1441 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     1501 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     1561 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     1621 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     1681 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     1741 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     1801 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     1861 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     1921 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     1981 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     2041 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     2101 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     2161 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     2221 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     2281 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     2341 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     2401 ggggcggcga ctgcagagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     2461 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     2521 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     2581 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     2641 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     2701 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     2761 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     2821 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     2881 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     2941 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     3001 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     3061 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     3121 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     3181 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     3241 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     3301 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     3361 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     3421 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     3481 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     3541 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     3601 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     3661 tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttctcaacaa taggcagtat
     3721 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3781 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3841 aaaattttcg ggtttattac tcggacagca gagatccagt ttggactagt ggagttccgc
     3901 gttacataac ttacgcaaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
     3961 acgtcaataa tgaccaatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
     4021 tgggtggagt atttacgcaa aactgcccac ttggcagtac atcaagtgta tcatttgcca
     4081 acaacgcccc ctattgacgt caatgacgca aaatggcccg cctggcatta tgcccagtac
     4141 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
     4201 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
     4261 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
     4321 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
     4381 cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc
     4441 catccacgct gttttgacct ccattcaaga caccggcggc cttaattaat gatcagacca
     4501 tggccgctgg ccgccccgtg cgtgggcccg agctggcgcc ccggaggctg ctgcagttgc
     4561 tgctgctggt actgctgggg ggcccgggcc ggggggcggc cttgagcggg aacgtgaccg
     4621 ggcctgggcc tcacagcgcc agcgggagct cgaggaggga cgtgccggtg accagccctc
     4681 cgccgccgct gctgagccac tgcggccggg ccgcccactg cgagcctttg cgctacaacg
     4741 tgtgcctggg ctcggcgctg ccctacggag ccaccaccac gctgctggct ggggactcgg
     4801 actcgcagga ggaagcgcac ggcaagctcg tgctctggtc cggcctccgg aatgcccccc
     4861 gctgctgggc agtgatccag cccctgctgt gtgctgtcta catgcccaag tgtgagaatg
     4921 accgagtgga gttgcccagc cgtaccctct gccaggccac ccgaggcccc tgtgccattg
     4981 tggagcggga gcgagggtgg cctgactttc tgcgttgcac accggaccac ttccctgaag
     5041 gctgcccaaa cgaggtacaa aacatcaagt tcaacagctc aggccaatgt gaagcaccct
     5101 tggtgcgaac agacaacccc aagagctggt atgaggacgt ggagggctgt gggattcagt
     5161 gtcagaaccc gctgttcacc gaggccgagc accaggacat gcacagctac atcgcagcct
     5221 tcggggcggt cactggtctc tgcacgctct tcacactggc cacctttgtg gctgactggc
     5281 ggaactccaa tcgctaccct gcggttattc tcttctatgt caatgcgtgt ttcttcgtgg
     5341 gcagcattgg ctggctggcc cagttcatgg atggtgcccg ccgagagatt gtttgccgag
     5401 cagatggcac catgagattt ggggagccca cctccagtga gaccctgtcc tgtgtcatca
     5461 tctttgtcat tgtgtactat gccttgatgg ctggagtagt ctggttcgtg gtcctcacct
     5521 atgcctggca cacctccttc aaagccctgg gcaccaccta ccagcctctc tcgggcaaga
     5581 catcctattt ccacctgctc acgtggtcac tcccctttgt cctcacggtg gcaatcctgg
     5641 ctgtggctca ggtagatgga gactccgtga gtggcatctg ttttgtaggc tacaagaact
     5701 atcggtaccg tgctggcttt gtcctggccc caattggcct ggtgcttatt gtgggaggct
     5761 acttcctcat cagaggggtc atgactctgt tctccatcaa gagcaaccac cctgggcttc
     5821 tgagtgagaa ggcagccagc aagatcaacg agaccatgct gcgcctgggc atttttggct
     5881 tcctggcctt tggctttgtg ctcatcacct tcagctgcca cttctatgac ttcttcaacc
     5941 aggctgagtg ggagcgtagc ttccgggact atgtgctatg ccaagccaac gtgaccatcg
     6001 ggctgcctac caagaagccc attcctgact gtgagatcaa gaatcggccc agcctcctgg
     6061 tggagaagat caatctattt gccatgtttg gcactggcat tgccatgagc acctgggtct
     6121 ggaccaaggc caccctgctc atctggaggc gcacctggtg caggttgact gggcacagtg
     6181 atgatgagcc caagagaatc aagaagagca agatgatcgc caaggccttc tctaagcggc
     6241 gtgagctgct gcagaacccg ggccaggagc tctccttcag catgcacact gtctcccatg
     6301 atggacctgt tgcgggtttg gcttttgacc tcaatgaacc ctcagctgac gtctcctctg
     6361 cctgggctca gcatgtcacc aagatggtgg ctcggagagg agccatattg ccccaggatg
     6421 tgtccgttac ccctgtggca actccagtgc caccagaaga acaagccaac atgtggctgg
     6481 ttgaggcaga gatctcccca gagttagaga agcgtttggg ccggaagaaa aagcggagga
     6541 agaggaagaa ggaggtgtgc cccttgaggc cagcccctga gcttcaccac tctgcccctg
     6601 ttcctgccac cagtgcagtt cctcggctgc ctcagctgcc tcggcagaag tgcctggtag
     6661 ctgcaaacgc ctggggaaca ggggagtcct gccgacaggg agcctggact ctagtctcca
     6721 accccttctg cccagagcct agtccccatc aagatccatt tctccctggt gcctcagccc
     6781 cccgggtctg ggctcagggc cgcctccagg ggctgggatc cattcattcc cgcactaacc
     6841 taatggaggc tgagatcttg gatgcagact cggacttcgc caccatggac tacaaagacc
     6901 atgacggtga ttataaagat catgacatcg attacaagga tgacgatgac aaggcggccg
     6961 cttagtaagt aagatccgcc cctctccctc ccccccccta acgttactgg ccgaagccgc
     7021 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     7081 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     7141 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     7201 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     7261 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     7321 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     7381 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     7441 gatctggggc ctcggtacac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     7501 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     7561 aaccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     7621 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     7681 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     7741 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     7801 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     7861 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     7921 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc taactcgaga gatcctaatc
     7981 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     8041 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     8101 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     8161 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     8221 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     8281 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     8341 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     8401 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     8461 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     8521 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga
     8581 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     8641 gaagggctaa ttcactccca acgaagacaa gatcacctgc aggacaggcg cgccctgctt
     8701 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     8761 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     8821 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     8881 aaaatctcta gcacccgggc gattaaggaa agggctagat cattcttgaa gacgaaaggg
     8941 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     9001 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     9061 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     9121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     9181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     9241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     9301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     9361 ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     9421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     9481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     9541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     9601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     9661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     9721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     9781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     9841 gcgtgggtct cgcggtatca ttgca
//
