LOCUS       sequence-32533-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pENTR4_FLAG/HA_FUS_His6
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             135..161
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             569..580
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgataag caatgctttt
       61 ttataatgcc aactttgtac aaaaaagcag gctccaccat gaatcccatg gactacaagg
      121 acgacgatga caagtaccct tatgacgtgc ccgattacgc tggatccgaa ttcgagctcc
      181 gtcgacccat ggcctcaaac gattataccc aacaagcaac ccaaagctat ggggcctacc
      241 ccacccagcc cgggcagggc tattcccagc agagcagtca gccctacgga cagcagagtt
      301 acagtggtta tagccagtcc acggacactt caggatatgg ccagagcagc tattcttctt
      361 atggccagag ccagaacaca ggctatggaa ctcagtcaac tccccaggga tatggctcga
      421 ctggcggcta tggcagtagc cagagctccc aatcgtctta cgggcagcag tcctcctacc
      481 ctggctatgg ccagcagcca gctcccagca gcacctcggg aagttacggt agcagttctc
      541 agagcagcag ctatgggcag ccccagagtg ggagctacag ccagcagcct agctatggtg
      601 gacagcagca aagctatgga cagcagcaaa gctataatcc ccctcagggc tatggacagc
      661 agaaccagta caacagcagc agtggtggtg gaggtggagg tggaggtgga ggtaactatg
      721 gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat ggcaatcaag
      781 accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgag gccgcgcagg
      841 gtggcagtgt gcggcggcgg cg
//
