LOCUS       sequence-325333-       10123 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SmoM2-FLAG-Ub.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: GP735
FEATURES             Location/Qualifiers
     source          1..10123
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        85..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    440..557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(853..897)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1082..1315
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1812..1937
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1984..2617
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(2809..2828)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2878..2894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2878..2894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2891..2913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2902..2918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2926..2932,2933..2950,2951..2956))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2971..2992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3232..3249)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3403..3991)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(4162..4953,4954..5022))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4785..4804
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5023..5127)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5195..5213
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             5260..5440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5588..6176
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5694..5705
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6103..6123
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6190..6588)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(6589..6597,6598..6600,6601..7174)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6762..6781)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6991..7008
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(7197..7424)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(7197..7424)
                     /codon_start=1
                     /gene="Homo sapiens UBC"
                     /product="human ubiquitin C"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(7458..7523)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      7523..7532
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(9983..10007)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(join(10004..10123,1..84))
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(10033..10053)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
       61 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
      121 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccattttgc
      181 gtcattgacg tcaatagggg gcgttgttgg caaatgatac acttgatgta ctgccaagtg
      241 ggcagttttg cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
      301 tatgggaaca ttggtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
      361 cgggccattt tgcgtaagtt atgtaacgcg gaactccact agtccaaact ggatctctgc
      421 tgtccgagta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
      481 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
      541 ccccttttct tttaaaattg tggatgaata ctgcctattg ttgagaattc ggcgataccg
      601 tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac cttcttcttc
      661 tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
      721 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
      781 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
      841 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
      901 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
      961 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     1021 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     1081 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     1141 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     1201 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     1261 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     1321 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     1381 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     1441 tcctcctcca ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt cttacttttg
     1501 ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc tatcctttga
     1561 tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc tgatcctgtc
     1621 tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt ttctaacagg
     1681 ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg ttttaattta
     1741 tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct aattctcccc
     1801 cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct agtcaaaatt
     1861 tttggcgtac tctgcagtcg ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga
     1921 gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct gttcgggcgc
     1981 cactgctaga gattttccac actgactaaa agggtctgag ggatctctag ttaccagagt
     2041 cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat tgaggcttaa
     2101 gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct aaccagagag
     2161 acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg ccactcccca
     2221 gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt gtagcaagct
     2281 cgatatcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg atgaaatgct
     2341 aggcggctgt caaacctcca ctctaacact tctctctccg ggtcatccat cccatgcagg
     2401 ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac cttatctggc
     2461 tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac ccctggccct
     2521 ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt ggtagatcca cagatcaagg
     2581 atatcttgtc ttctttggga gtgaattagc ccttccaact actaagtttg tagtacatat
     2641 ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg tttagagtaa
     2701 atgaaaccac aggtaattgg cagtggtaat agggtatggg gtgggaagtt tgggatgatt
     2761 ttggttagct tgagttatcc agttgatcca gacatgataa gatacattga tgagtttgga
     2821 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaattcg taatcatgtc
     2881 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2941 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3001 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cacggggagc tttttgcaaa
     3061 agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     3121 cctcggcctc tgcataaata aaaaaaatta gtcagccatg agcttgctgc attaatgaat
     3181 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     3241 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     3301 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     3361 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     3421 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3481 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3541 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3601 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3661 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3721 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3781 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3841 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3901 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3961 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4021 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     4081 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     4141 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     4201 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     4261 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     4321 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     4381 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     4441 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     4501 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     4561 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     4621 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     4681 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     4741 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     4801 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     4861 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     4921 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     4981 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     5041 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     5101 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     5161 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     5221 tcttcaagaa tgatctagcc ctttccttaa tcgcccgggt gctagagatt ttccacactg
     5281 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     5341 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     5401 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcaggg
     5461 cgcgcctgtc ctgcaggtga tcttgtcttc gttgggagtg aattagccct tccagtcccc
     5521 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     5581 gtaccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga
     5641 cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg
     5701 gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc
     5761 agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt gcccaacagc
     5821 cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt ggcaataggg
     5881 agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc ccaaccagtg
     5941 ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac gggccacaac
     6001 tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa agccatacgg
     6061 gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt aaaaggagca
     6121 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattagga
     6181 tctctcgagt tagccctccc acacataacc agagggcagc aattcacgaa tcccaactgc
     6241 cgtcggctgt ccatcactgt ccttcactat ggctttgatc ccaggatgca gatcgagaag
     6301 cacctgtcgg caccgtccgc aggggctcaa gatgcccctg ttctcatttc cgatcgcgac
     6361 gatacaagtc aggttgccag ctgccgcagc agcagcagtg cccagcacca cgagttctgc
     6421 acaaggtccc ccagtaaaat gatatacatt gacaccagtg aagatgcggc cgtcgctaga
     6481 gagagctgcg ctggcgacgc tgtagtcttc agagatgggg atgctgttga ttgtagccgt
     6541 tgctctttca atgagggtgg attcttcttg agacaaaggc ttggccatgg ttgtggccat
     6601 attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg cctagacgtt
     6661 tttttaacct cgactaaaca catgtaaagc atgtgtaccg aggccccaga tcagatccca
     6721 tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt gaggagagcc
     6781 atttgactct ttccacaact atccaactca caacgtggca ctggggttgt gccgcctttg
     6841 caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca ggtggggggt
     6901 tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct tccagaggaa
     6961 ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg gaaagacccc
     7021 taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg ccaaaagacg
     7081 gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc aagcggcttc
     7141 ggccagtaac gttagggggg gggagggaga ggggcggatc ttacttacta agctcaacca
     7201 cctctgagac ggaggaccag gtgcagggtg gactctttct ggatgttgta gtcagacagg
     7261 gtgcgtccat cttccagctg tttcccagca aagatcaacc tctgctggtc aggagggatg
     7321 ccttccttgt cttggatctt tgccttgaca ttctcaatgg tgtcactcgg ctccacttcg
     7381 agagtgatgg tcttaccagt cagggtcttc acgaagatct gcatggtcga cccaccacca
     7441 ccaccattga gatctgcctt gtcatcgtca tccttgtaat cgatgtcatg atctttataa
     7501 tcaccgtcat ggtctttgta gtccatggtg gcgaagtccg agtctgcatc caagatctca
     7561 gcctccatta ggttagtgcg ggaatgaatg gatcccagcc cctggaggcg gccctgagcc
     7621 cagacccggg gggctgaggc accagggaga aatggatctt gatggggact aggctctggg
     7681 cagaaggggt tggagactag agtccaggct ccctgtcggc aggactcccc tgttccccag
     7741 gcgtttgcag ctaccaggca cttctgccga ggcagctgag gcagccgagg aactgcactg
     7801 gtggcaggaa caggggcaga gtggtgaagc tcaggggctg gccccaaggg gcacacctcc
     7861 ttcttcctct tcctccgctt tttcttccgg cccaaacgct tctctaactc tggggagatc
     7921 tctgcctcaa ccagccacat gttggcttgt tcttctggtg gcactggagt tgccacaggg
     7981 gtaacggaca catcctgggg caatatggct cctctccgag ccaccatctt ggtgacatgc
     8041 tgagcccagg cagaggagac gtcagctgag ggttcattga ggtcaaaagc caaacccgca
     8101 acaggtccat catgggagac agtgtgcatg ctgaaggaga gctcctggcc cgggttctgc
     8161 agcagctcac gccgcttaga gaaggccttg gcgatcatct tgctcttctt gattctcttg
     8221 ggctcatcat cactgtgccc agtcaacctg caccaggtgc gcctccagat gagcagggtg
     8281 gccttggtcc agaccaaggt gctcatggca atgccagtgc caaacatggc aaatagattg
     8341 atcttctcca ccaggaggct gggccgattc ttgatctcac agtcaggaat gggcttcttg
     8401 gtaggcagcc cgatggtcac gttggcttgg catagcacat agtcccggaa gctacgctcc
     8461 cactcagcct ggttgaagaa gtcatagaag tggcagctga aggtgatgag cacaaagcca
     8521 aaggccagga agccaaaaat gcccaggcgc agcatggtct cgttgatctt gctggctgcc
     8581 ttctcactca gaagcccagg gtggttgctc ttgatggaga acagagtcat gacccctctg
     8641 atgaggaagt agcctcccac aataagcacc aggccaattg gggccaggac aaagccagca
     8701 cggtaccgat agttcttgta gcctacaaaa cagatgccac tcacggagtc tccatctacc
     8761 tgagccacag ccaggattgc caccgtgagg acaaagggga gtgaccacgt gagcaggtgg
     8821 aaataggatg tcttgcccga gagaggctgg taggtggtgc ccagggcttt gaaggaggtg
     8881 tgccaggcat aggtgaggac cacgaaccag actactccag ccatcaaggc atagtacaca
     8941 atgacaaaga tgatgacaca ggacagggtc tcactggagg tgggctcccc aaatctcatg
     9001 gtgccatctg ctcggcaaac aatctctcgg cgggcaccat ccatgaactg ggccagccag
     9061 ccaatgctgc ccacgaagaa acacgcattg acatagaaga gaataaccgc agggtagcga
     9121 ttggagttcc gccagtcagc cacaaaggtg gccagtgtga agagcgtgca gagaccagtg
     9181 accgccccga aggctgcgat gtagctgtgc atgtcctggt gctcggcctc ggtgaacagc
     9241 gggttctgac actgaatccc acagccctcc acgtcctcat accagctctt ggggttgtct
     9301 gttcgcacca agggtgcttc acattggcct gagctgttga acttgatgtt ttgtacctcg
     9361 tttgggcagc cttcagggaa gtggtccggt gtgcaacgca gaaagtcagg ccaccctcgc
     9421 tcccgctcca caatggcaca ggggcctcgg gtggcctggc agagggtacg gctgggcaac
     9481 tccactcggt cattctcaca cttgggcatg tagacagcac acagcagggg ctggatcact
     9541 gcccagcagc ggggggcatt ccggaggccg gaccagagca cgagcttgcc gtgcgcttcc
     9601 tcctgcgagt ccgagtcccc agccagcagc gtggtggtgg ctccgtaggg cagcgccgag
     9661 cccaggcaca cgttgtagcg caaaggctcg cagtgggcgg cccggccgca gtggctcagc
     9721 agcggcggcg gagggctggt caccggcacg tccctcctcg agctcccgct ggcgctgtga
     9781 ggcccaggcc cggtcacgtt cccgctcaag gccgcccccc ggcccgggcc ccccagcagt
     9841 accagcagca gcaactgcag cagcctccgg ggcgccagct cgggcccacg cacggggcgg
     9901 ccagcggcca tggtctgatc attaattaag gccgccggtg tcttgaatgg aggtcaaaac
     9961 agcgtggatg gcgtctccag gcgatctgac ggttcactaa acgagctctg cttatataga
    10021 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
    10081 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc att
//
