LOCUS       sequence-325345-        9766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Smo-FLAG K434, 444R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9766)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: GP824
FEATURES             Location/Qualifiers
     source          1..9766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      302..890
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1044..1061
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1301..1322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1337..1342,1343..1360,1361..1367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1375..1391
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1380..1402
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1399..1415
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1399..1415
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             1676..2309
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2356..2481
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2978..3211
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3396..3440
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3736..3853
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    3902..3950
                     /note="synthetic polyadenylation signal"
     misc_feature    3964..4055
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4004..4023
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    4070..4204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4107..4126)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             4400..4795
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(4796..5369,5370..5372,5373..5381)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4962..4979)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5189..5208
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      7768..7777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7777..7842
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7883..8471
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7936..7956)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8354..8365)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8619..8799
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8846..8864)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8932..9036
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9037..9105,9106..9766,1..131)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9255..9274)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
       61 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      121 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      181 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      241 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      301 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      361 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      421 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      481 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      541 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      601 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      661 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      721 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      781 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      841 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      901 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      961 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1021 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     1081 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
     1141 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     1201 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
     1261 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     1321 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     1381 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
     1441 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1501 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     1561 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
     1621 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagttggaa
     1681 gggctaattc actcccaaag aagacaagat atccttgatc tgtggatcta ccacacacaa
     1741 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     1801 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     1861 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     1921 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     1981 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     2041 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     2101 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     2161 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     2221 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     2281 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     2341 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     2401 ggcgaggggc ggcgactgca gagtacgcca aaaattttga ctagcggagg ctagaaggag
     2461 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     2521 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     2581 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     2641 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     2701 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     2761 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     2821 gccggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     2881 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     2941 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     3001 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     3061 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     3121 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     3181 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     3241 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     3301 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     3361 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     3421 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     3481 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     3541 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     3601 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     3661 acagatccat tcgattagtg aacggatctc gacggtatcg ccgaattctc aacaataggc
     3721 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     3781 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     3841 aattcaaaat tttcgggttt attactcgga cagcagagat ccagtttgga ctagtggccg
     3901 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
     3961 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
     4021 ccccagtgca agtgcaggtg ccagaacatt tctctatcga taggtaccga acttgtttat
     4081 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4141 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4201 gatccccggg aacagattcg cgactagtgg acacacagga taggggtggg cagcatgagg
     4261 ggggccagag ggagaggggg cagagctggg ctggacgagg ggacaccccg ccccaatggg
     4321 gcgtgacgag ctgccagccc aggctccggg gcacgtaaaa gcggggctgt gagaccggag
     4381 agcacggagc gacattaata tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     4441 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     4501 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     4561 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     4621 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     4681 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     4741 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggccccct
     4801 ctccctcccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4861 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4921 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4981 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     5041 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     5101 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     5161 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     5221 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
     5281 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     5341 ggttttcctt tgaaaaacac gatgataata tggccacaac catagaccat ggccgctggc
     5401 cgccccgtgc gtgggcccga gctggcgccc cggaggctgc tgcagttgct gctgctggta
     5461 ctgctggggg gcccgggccg gggggcggcc ttgagcggga acgtgaccgg gcctgggcct
     5521 cacagcgcca gcgggagctc gaggagggac gtgccggtga ccagccctcc gccgccgctg
     5581 ctgagccact gcggccgggc cgcccactgc gagcctttgc gctacaacgt gtgcctgggc
     5641 tcggcgctgc cctacggagc caccaccacg ctgctggctg gggactcgga ctcgcaggag
     5701 gaagcgcacg gcaagctcgt gctctggtcc ggcctccgga atgccccccg ctgctgggca
     5761 gtgatccagc ccctgctgtg tgctgtctac atgcccaagt gtgagaatga ccgagtggag
     5821 ttgcccagcc gtaccctctg ccaggccacc cgaggcccct gtgccattgt ggagcgggag
     5881 cgagggtggc ctgactttct gcgttgcaca ccggaccact tccctgaagg ctgcccaaac
     5941 gaggtacaaa acatcaagtt caacagctca ggccaatgtg aagcaccctt ggtgcgaaca
     6001 gacaacccca agagctggta tgaggacgtg gagggctgtg ggattcagtg tcagaacccg
     6061 ctgttcaccg aggccgagca ccaggacatg cacagctaca tcgcagcctt cggggcggtc
     6121 actggtctct gcacgctctt cacactggcc acctttgtgg ctgactggcg gaactccaat
     6181 cgctaccctg cggttattct cttctatgtc aatgcgtgtt tcttcgtggg cagcattggc
     6241 tggctggccc agttcatgga tggtgcccgc cgagagattg tttgccgagc agatggcacc
     6301 atgagatttg gggagcccac ctccagtgag accctgtcct gtgtcatcat ctttgtcatt
     6361 gtgtactatg ccttgatggc tggagtagtc tggttcgtgg tcctcaccta tgcctggcac
     6421 acctccttca aagccctggg caccacctac cagcctctct cgggcaagac atcctatttc
     6481 cacctgctca cgtggtcact cccctttgtc ctcacggtgg caatcctggc tgtggctcag
     6541 gtagatggag actccgtgag tggcatctgt tttgtaggct acaagaacta tcggtaccgt
     6601 gctggctttg tcctggcccc aattggcctg gtgcttattg tgggaggcta cttcctcatc
     6661 agaggggtca tgactctgtt ctccatcagg agcaaccacc ctgggcttct gagtgagagg
     6721 gcagccagca agatcaacga gaccatgctg cgcctgggca tttttggctt cctggccttt
     6781 ggctttgtgc tcatcacctt cagctgccac ttctatgact tcttcaacca ggctgagtgg
     6841 gagcgtagct tccgggacta tgtgctatgc caagccaacg tgaccatcgg gctgcctacc
     6901 aagaagccca ttcctgactg tgagatcaag aatcggccca gcctcctggt ggagaagatc
     6961 aatctatttg ccatgtttgg cactggcatt gccatgagca cctgggtctg gaccaaggcc
     7021 accctgctca tctggaggcg cacctggtgc aggttgactg ggcacagtga tgatgagccc
     7081 aagagaatca agaagagcaa gatgatcgcc aaggccttct ctaagcggcg tgagctgctg
     7141 cagaacccgg gccaggagct ctccttcagc atgcacactg tctcccatga tggacctgtt
     7201 gcgggtttgg cttttgacct caatgaaccc tcagctgacg tctcctctgc ctgggctcag
     7261 catgtcacca agatggtggc tcggagagga gccatattgc cccaggatgt gtccgttacc
     7321 cctgtggcaa ctccagtgcc accagaagaa caagccaaca tgtggctggt tgaggcagag
     7381 atctccccag agttagagaa gcgtttgggc cggaagaaaa agcggaggaa gaggaagaag
     7441 gaggtgtgcc ccttgaggcc agcccctgag cttcaccact ctgcccctgt tcctgccacc
     7501 agtgcagttc ctcggctgcc tcagctgcct cggcagaagt gcctggtagc tgcaaacgcc
     7561 tggggaacag gggagtcctg ccgacaggga gcctggactc tagtctccaa ccccttctgc
     7621 ccagagccta gtccccatca agatccattt ctccctggtg cctcagcccc ccgggtctgg
     7681 gctcagggcc gcctccaggg gctgggatcc attcattccc gcactaacct aatggaggct
     7741 gagatcttgg atgcagactc ggacttcgcc accatggact acaaagacca tgacggtgat
     7801 tataaagatc atgacatcga ttacaaggat gacgatgaca aggcggccgc ttagtaagta
     7861 agatccgaac tcgagagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac
     7921 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     7981 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     8041 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     8101 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     8161 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     8221 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     8281 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     8341 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     8401 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     8461 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     8521 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     8581 acctgcagga caggcgcgcc ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     8641 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     8701 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     8761 ccctcagacc cttttagtca gtgtggaaaa tctctagcac ccgggcgatt aaggaaaggg
     8821 ctagatcatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     8881 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     8941 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     9001 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     9061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     9121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     9181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     9241 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
     9301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     9361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     9421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     9481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     9541 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     9601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     9661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     9721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatca
//
